Figure 6.

Tissue morphology of skeletal muscle, liver, white adipose, and brown adipose tissues shown by hematoxylin and eosin staining. Lipid accumulation in skeletal muscle was not higher in the HFS diets compared to the LFD. A small increase in hepatic lipid content with the HFS was attenuated by botanical supplementation. Lipid accumulation in perigonadal (gWAT) and inguinal (iWAT) white adipose tissue was increased by adipocyte hypertrophy in all HFS-fed groups. Brown-fat lipid accumulation occurred in unilocular and multilocular lipid droplets in the females fed the HFS diet but was unilocular and multilocular droplets in the HFS alone diet and predominantly multilocular lipid droplets in the botanical supplemented HFS-fed diets. The images are representative of images from four mice/group. The black bar length represents 100 µm.