Skip to main content
. 2020 Aug 30;9(1):1808424. doi: 10.1080/2162402X.2020.1808424

Figure 3.

Figure 3.

The conditioned medium (CM) from slanMo and NK cell co-cultures leads to a growth arrest in melanoma cells. (A) Growth curve of SK-Mel-28 untreated (Control medium), or treated with R848-activated slanMo/NK cell co-culture conditioned medium (CM), R848-activated slanMo-conditioned medium (slanMo CM), or R848-activated NK cell-conditioned medium (NK cell CM). The melanoma cells were incubated for 3–4 d with the respective CM. After CM treatment, the cells were washed and cultured in normal control medium. Cumulative data from 2 (CM) or 3 donors (slanMo CM and NK cell CM). (B) Cytokine screen of conditioned medium from different cultures either unstimulated or stimulated for 18 hours with 1 µg/ml R848 or 100 ng/ml LPS. (C) Growth curve of SK-Mel-28 and Ma-Mel-86b after treatment with recombinant TNF-α and IFN-γ (10 ng/ml and 100 ng/ml, respectively). (D) Growth curve of SK-Mel-28 after incubation with co-culture CM with neutralizing antibodies against TNF-α/IFN-γ (α-TNF-α/IFN-γ) or respective isotype controls (Iso) (each 10 µg/ml). Cumulative data from 12 donors.