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. 2020 Jul 29;39(36):5876–5887. doi: 10.1038/s41388-020-01399-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Western blot analyses for AKT and ACTIN (a) and the growth rates (b) of control (shCTL), PIERCE1 KD (shP#1 and shP#3), and PIERCE1 KD with overexpression of myristoylated AKT (shP#1+AKT and shP#3+AKT) cell lines in A549 cell line. A representative image of xenograft tumors of cell lines 35 days after s.c. injection into nude mice (c) and their growth rates (d). White bar indicates 1 cm. Error bars represent ±SD. Statistical analysis was performed using one-way ANOVA for b and d. *P < 0.05; **P < 0.01.