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. 2020 Jul 29;39(36):5876–5887. doi: 10.1038/s41388-020-01399-5

Fig. 6. PIERCE1 KO inhibits lung tumorigenesis in vivo.

Fig. 6

a Representative photos of four-month-old WT and PIERCE1 KO mouse lungs in KRASLA2 background. Black bars indicate 1 cm. Average numbers of tumors (b) and maximum tumor size (c) of WT (n = 12) and PIERCE1 KO (n = 12) 4-month-old mouse lungs in KRASLA2 background. d The survival curve of WT (n = 16) and PIERCE1 KO (n = 18) mice in KRASLA2 background. e A representative photo of oil or tamoxifen-injected mouse lungs in KRASLA2;PIERCE1f/f;SPC-CreERT2 background. White bar indicates 1 cm. f Average number of tumors at the surface of lungs of oil or tamoxifen-injected mice (n = 8 for oil-injected CreTg, n = 3 for Tam-injected Crenon-Tg, and n = 8 for Tam-injected CreTg). *P < 0.05; **P < 0.01; ***P < 0.0001. Percentages of Ki67 positive cells per given field (g) and representative immunohistochemistry images of cleaved caspase-3 (c-casp-3, h) in oil- (n = 5) and tamoxifen-treated (n = 5) mouse lung tumors. Black bar indicates 100 μm. Error bars represent ±SD. Statistical analysis was performed using unpaired t test. **P < 0.01. i Immunohistochemistry analyses for PIERCE1 and pAKT (S473) expressions in human lung cancer samples. Scale bars indicate 100 μm.