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. 2020 Aug 20;23(9):101472. doi: 10.1016/j.isci.2020.101472

Figure 5.

Figure 5

Increased Susceptibility of Adamts18−/− Mice to Bleomycin-Induced Lung Fibrosis

(A) Log rank curves of 8-week-old Adamts18+/+ and Adamts18−/− male mice challenged with bleomycin (n = 19/group, ∗p < 0.05).

(B) Representative images of lung sections of saline-treated (upper panels) or bleomycin-treated (lower panels) mice. Increased inflammation in Adamts18−/− lung sections is revealed by H&E staining (lower panel, white arrows). Scale bar, 100 μm.

(C) Ashcroft scoring of lung fibrosis in mice 23 days after treatment with bleomycin or saline. Results are expressed as mean ± SD (n = 4/group, ∗∗p < 0.01, Student's t test).

(D) Masson's trichrome staining for aggregated collagen deposition in lung sections of bleomycin-treated Adamts18−/− mice. Scale bar, 100 μm.

(E) Relative mRNA levels of Adamts18 in lungs of saline or bleomycin-treated mice determined by quantitative real-time RT-PCR. P1 mouse lung serves as the negative control. Data are expressed as mean ± SEM (n = 3).