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. 2020 Aug 20;23(9):101472. doi: 10.1016/j.isci.2020.101472

Figure 7.

Figure 7

Restoration of Adamts18−/− Lung Branching Morphogenesis by Fbn1 or Fbn2 Sense (Control) and Antisense Oligonucleotides (ASODN) in Lung Explants

(A and D) E11.5 lungs were cultured in vitro and photographed at 0, 24, 48, and 72 h. 0.5 μM Fbn1 ODN or 1 μM Fbn2 ODN was added to the cultures every 24 h. Scale bar, 100 μm.

(B and E) Branch number of lungs cultured for 72 h.

(C and F) Lengths of L1-L5 and R1-R5 bronchi of 72 h-cultured explants. SODN, sense oligonucleotides. ASODN, antisense oligonucleotides. Results are expressed as mean ± SD, n = 3 (∗p < 0.05). These experiments were repeated independently at least three times.