KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse anti-GFP clone 3E6 | Thermo Fisher Scientific |
A-11120, AB_221568 |
| Alexa Fluor 488 donkey anti-mouse | Thermo Fisher Scientific | A-21202, AB_141607 |
| Bacterial and Virus Strains | ||
| Biological Samples | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| All-trans-retinal | Sigma-Aldrich | R2500 |
| Picrotoxin | Sigma-Aldrich | P1675 |
| Mecamylamine | Sigma-Aldrich | M9020 |
| Dopamine | Alfa Aesar | A11136–06 |
| ATP disodium salt hydrate | Sigma-Aldrich | A2383 |
| Critical Commercial Assays | ||
| Deposited Data | ||
| Experimental Models: Cell Lines | ||
| Experimental Models: Organisms/Strains | ||
| D. melanogaster: w1118/Dp(2;Y), P{hs-hid}Y | Bloomington Drosophila Stock Center (BDSC) | BDSC: 24638 |
| D. m.: wild-type Canton S | Keleman et al., 2012 | Flybase: FBsn0000274 |
| D. m.: w*; P{UAS-TrpA1(B).K}attP16 | BDSC | BDSC: 26263 |
| D. m.: w1118;; P{ple-GAL4.F}3 | Friggi-Grelin et al., 2003 | Flybase: FBti0072936 |
| D. m.: w1118;; P{VT002857-Gal4}attP2 | Vienna Drosophila Resource Center (VDRC) | VDRC: 200494 |
| D. m.: y*, w*;; P{w[+mW.hs]=GawB}mura[NP5945]/TM6, P{w[-]=UAS-lacZ.UW23–1}UW23–1 | Kyoto Drosophila Genomics and Genetic Resources | DGGR: 105062 |
| D. m.: DopR2-RNAi: w1118; P{KK110947}VIE-260B | VDRC | VDRC: 105324 |
| D. m.: P1-B-Gal4: w1118;; P{GMR71G01-GAL4}attP2 | BDSC | BDSC: 39599 |
| D. m.: w1118; P{w[+mC]=UAS-Dcr-2.D}2 | BDSC | BDSC: 24650 |
| D. m.: w1118;; P{w[+mC]=UAS-Dcr-2.D}10 | BDSC | BDSC: 24651 |
| D. m.: w1118; P{UAS-eGFP-Kir2.1}/CyO | Baines et al., 2001 | Flybase: FBtp0110871 |
| D. m.: w*;; P{tubP-GAL80ts}2/TM2 | BDSC | BDSC: 7017 |
| D. m.: AbdBLDN-Gal4: y1, w*;; P{GawB}Abd-BLDN/TM6B, Tb1 | BDSC | BDSC: 55848 |
| D. m.: mAL-S-Gal4: w1118;; P{GMR43D01-GAL4}attP2 | BDSC | BDSC: 64345 |
| D. m.: mAL-R-LexA: w1118; P{GMR25E04-lexA}attP40 | BDSC | BDSC: 61524 |
| D. m.: w1118; P{LexAop-IVS-Syn21-opGCaMP6s}su(Hw)attP5 | Gift from B.D. Pfeiffer and D.J. Anderson | N/A |
| D. m.: w1118; P{10xUAS-IVS-Syn21-Chrimson-tdTomato-M19} su(Hw)attP5 | Gift from B.D. Pfeiffer and D.J. Anderson | N/A |
| D. m.: w1118;; P{UAS-Rnor\P2rx2.L} | Lima and Miesenböck, 2005 | Flybase: FBtp0021869 |
| D. m.: P1-B-LexA: w1118; P{GMR71G01-LexA}attP40 | BDSC | BDSC: 54733 |
| D. m.: w1118;; P{lexAop-P2X2.Y} | Yao et al., 2012 | Flybase: FBtp0093385 |
| D. m.: w1118; P{UAS-IVS-GCaMP6s}attP40 | BDSC | BDSC: 42746 |
| D. m.: w1118; P{UAS-ASAP1}attP40 | BDSC | BDSC: 65412 |
| D. m.: w1118; PBac{UAS-ASAP2s}VK00005 | BDSC | BDSC: 76247 |
| D. m.: y1, w*; PinYt/CyO; P{UAS-mCD8::GFP.L}LL6 | BDSC | BDSC: 5130 |
| D. m.: Rdl-RNAi-1: w1118; P{KK104293}VIE-260B | VDRC | VDRC: 100429 |
| D. m.: Rdl-RNAi-2: y1, v1;; P{TRiP.JF01227}attP2 | BDSC | BDSC: 31286 |
| D. m.: pHCl-RNAi: w1118; P{KK112646}VIE-260B | VDRC | VDRC: 103247 |
| D. m.: +;; DopR2attP | Keleman et al., 2012 | N/A |
| D. m.: Gαs-RNAi-1: y1 v1; P{TRiP.HMC03106}attP2 | BDSC | BDSC: 50704 |
| D. m.: Gαs-RNAi-2: y1 v1; P{TRiP.JF03255}attP2 | BDSC | BDSC: 29576 |
| D. m.: nAChRα3-RNAi-1: y1 v1; P{TRiP.HMJ23004}attP40/CyO | BDSC | BDSC: 61225 |
| D. m.: nAChRα3-RNAi-2: y1, v1;; P{TRiP.JF03255}attP2 | BDSC | BDSC: 27671 |
| D. m.: nAChRα6-RNAi-1: y1, sc*, v1; P{TRiP.HMC03623}attP40 | BDSC | BDSC: 52885 |
| D. m.: nAChRα6-RNAi-2: y1, v1; P{TRiP.HMJ21826}attP40/CyO | BDSC | BDSC: 57818 |
| D. m.: w1118;; P{UAS-IVS-Syn21-CsChrimson-tdTomato}attP2 | Hoopfer et al., 2015 | N/A |
| D. m.: w1118;; P{ple-GAL4.TH-C'}/Sb |
Liu et al., 2012 |
Flybase: FBtp0083572 |
| D. m.: w1118; P{ple-GAL4.TH-D'}/CyO | Liu et al., 2012 | Flybase: FBtp0083573 |
| D. m.: w1118;; P{ple-GAL4.TH-F}1/Sb |
Liu et al., 2012 |
Flybase: FBti0151785 |
| D. m.: w1118;; P{ple-GAL4.TH-G}1/Sb | Liu et al., 2012 | Flybase: FBti0151784 |
| D. m.: PPN1-S-Gal4: w1118; P{GMR56C09-GAL4}attP2 | BDSC | BDSC: 39145 |
| D. m.: w1118; P{LexAop-CD8::spGFP11}; P{UAS-CD4-spGFP1–10} | Gordon and Scott, 2009 | N/A |
| D. m.: w1118;; P{UAS-IVS-GtACR1}attP2 | Mohammad et al., 2017 | N/A |
| Oligonucleotides | ||
| Recombinant DNA | ||
| Software and Algorithms | ||
| 2-photon calcium-imaging analysis package (MATLAB) | Zhang et al., 2016 | https://github.com/CrickmoreRoguljaLabs/Ca2plus |
| ROI-based fluorescence quantification package (MATLAB) | Zhang et al., 2016 | https://github.com/CrickmoreRoguljaLabs/Blind-image-analysis |
| Courtship-initiation analysis package (MATLAB) | This paper | https://github.com/CrickmoreRoguljaLabs/tapping-codes |
| Simulation package for linear evidence accumulation (MATLAB) | This paper | https://github.com/CrickmoreRoguljaLabs/Tap_EvidenceAccumulation |
| Jeffrey’s 95% confidence interval (MATLAB) | This paper | https://github.com/CrickmoreRoguljaLabs/Jeffi |
| Two-sample Kolmogorov-Smirnov test (MATLAB) | This paper | https://github.com/CrickmoreRoguljaLabs/KSstat |
| Other | ||
| Phosphate Buffered Saline 10x | MediaTech | 46–013-CM |
| Triton X-100 | Sigma-Aldrich | X100 |
| Potato flakes | Carolina Biological Supply | 173200 |
| Nunc Petri Dishes | Thermo Scientific | 150318 |
| 655-nm LED | Luxeon Star LEDs | LXM3-PD01–0350 |
| 530-nm LED | Luxeon Star LEDs | LXML-PM01–0100 |
| Arduino MEGA | Arduino | A000067 |
| Raspberry PI Model B+ | Raspberry PI | Model B+ |