KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-6xHis lag antibody APC-conjugated | Abeam | Cat#ab72467; RRID: AB_1267596 |
| Anti-6xHis tag antibody PE-conjugated | Abeam | Cat#ab72579: RRID: AB_1267597 |
| Goat anti-human IgG FC (FITC) | Abeam | Cat#ab97224: RRID: AB_10680849 |
| Bacterial and Virus Strains | ||
| E.coli BL21(DE3) pLysS | Promega | N/A |
| E.coli methionine auxotroph B834(DE3) | Novagen | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| PEI, MW 25000 | Polysciences Inc. | Cat#23966–1 |
| Recombinant human HVEM Fc | R&D systems | Cat#356-HV/CF |
| Recombinant human BTLA His-lag | R&D systems | Cat#9235-BT-050 |
| Recombinant human CP160His-tag | R&D systems | Cat#6177-CD-0S0 |
| Critical Commercial Assays | ||
| MCSG crystallization suite | Anatrace | MCSG-1 |
| MCSG crystallization suite | Anatrace | MCSG-2 |
| MCSG crystallization suits | Anatrace | MCSG-3 |
| MCSG crystallization suite | Anatrace | MCSG-4 |
| Deposited Data | ||
| Crystal structure of gD:HVEM complex | (Carfi et al., 2001) | PDB: 1JMA |
| Crystal structure of BLA:HVEM complex | (Compaan et al., 2005) | PDB:2AW2 |
| Crystal structure oi TNF:TNFR2 complex | (Mukai et al., 2010) | PDB:3ALQ |
| Crystal structure of equine lentivirus recpetor 1 | (Qian et al. 2015) | PDB:3WVT |
| Crystal structure of HVEM | (Liu et al., 2015) | PDB:4FHQ |
| Crystal structure of Fab:LTα1β2:LTβR complex | (Sudhamsu et al., 2013) | PDB:4MXW |
| Crystal structure of Fab:CD40 complex | (Yu et al., 2018) | PDB:6FAX |
| Crystal structure of sc_CD160:HVEM | This paper | PDB:6NG3 |
| Crystal structure of CD160 | This paper | PDB:6NG9 |
| Crystal structure of SeMet V58M CD160 | This paper | PDB:6NGG |
| Experimental Models: Cell Lima | ||
| Drosophila S2 | Thermo Fisher | N/A |
| HEK293 | Thermo Fisher | N/A |
| Recombinant UNA | ||
| Human CD160-pET3a | This paper | N/A |
| Human BTLA-pET3a | This paper | N/A |
| Human single chain CD16D-HVEM-pMT-Bip-V5-His | This paper | N/A |
| Human CD160-pMT-Blp-V5-His | This paper | N/A |
| Human CD160-pmCherry-N1 | This paper | N/A |
| Human HVEM pGFP-N1 | This paper | N/A |
| HSV1-gD-plRES-acGFP | This paper | N/A |
| Software and Algorithms | ||
| HHL2000 | (Otwinowski and Minor, 1997) | http://www.hkl-xray.com/ |
| SHELX | (Sheldrick, 2010) | http://shelx.uni-goettingen.de/ |
| CCP4 | (Winn et al., 2011) | https://www.ccp4.ac.uk/ |
| COOT | (Emsley et al., 2010) | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ |
| ARP/wARP | (Winn et al., 2011) | http://www.embt-hamburg.de/ARP/ |
| REFMAC5 | (Winn et al., 2011) | http://www.ccp4.ac.uk/html/refmac5.html |
| Pymol | Molecular Graphics System, Schrodinger, LLC | https://pyimol.org/2/ |
| CLUSTALW | (Thompson et al., 1994) | https://www.genome.jp/tools-bin/clustalw |
| ESRript 3.0 | (Robert and Gouet, 2014) | https://espript.ibcp.fr/ESPript/ESPript/ |
| PDBePISA | (Krissinel and Henrick, 2007) | http://wwiw.ebi.ac.uk/pdbe/pisa/ |
| Prism 5 | Graphpad | https://www.graphpad.com/scientific-software/prism/ |