KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Chicken polyclonal anti-GFP | Abcam | ab13970; RRID: AB_300798 |
| Rabbit polyclonal anti-c-Fos | Synaptic Systems | 226003; RRID: AB_2231974 |
| Rabbit monoclonal anti-c-Fos | Cell Signaling | 2250S; RRID: AB_2247211 |
| Rabbit polyclonal anti-RFP | Rockland | 600-401-379; RRID: AB_2209751 |
| Rat monoclonal anti-mCherry (16D7) | ThermoFisher | M11217; RRID: AB_2536611 |
| Chicken polyclonal anti-mCherry | Abcam | ab205402; RRID: AB_2722769 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Clozapine N-oxide | Sigma-Aldrich | C0832-5MG |
| Critical Commercial Assays | ||
| RNAscope Multiplex Fluorescent Assay V2 | Advanced Cell Diagnostics (ACD Bio) | 323100 |
| RNAscope Probe- Mm-Slc32a1 | ACD Bio | 319191-C1 |
| RNAscope Probe- mCherry-C2 | ACD Bio | 431201-C2 |
| RNAscope Probe- EGFP | ACD Bio | 400281-C1 |
| RNAscope Probe- tdTomato-C2 | ACD Bio | 317041-C2 |
| Experimental Models: Organisms/Strains | ||
| Mouse: Vgat-IRES-Cre: Slc32a1tm2(cre)Lowl/J | The Jackson Laboratory | 016962; RRID: ISMR_JAX:016962 |
| Mouse: Rosa26-LSL-EGFPL10a: B6.129S4-Gt(ROSA)26Sortm1(CAG-EGFP/Rp/10a,birA)Wtp/J | The Jackson Laboratory | 022367; RRID: IMSR_JAX:022367 |
| Mouse: C57BL/6J | The Jackson Laboratory | 000664; RRID: IMSR_JAX:000664 |
| Mouse: TRAP2: Fostm2.1(icre/ERT2)Luo/J | The Jackson Laboratory | 030323; RRID: IMSR_JAX:030323 |
| Mouse: Ai14: B6;129S6-Gt(ROSA)26Sortm14(CAG-tdTomato)/Hze/J | The Jackson Laboratory | 007908; RRID: IMSR_JAX:007908 |
| Recombinant DNA | ||
| AAV5-EF1a-DIO-hChR2(H134R)-EYFP | UNC Vector Core | N/A |
| AAV5-EF1a-DIO-EYFP | UNC Vector Core | N/A |
| AAV5-hSyn-DIO-hM3D(Gq)-mCherry | Addgene | 44361 |
| AAV5-hSyn-DIO-hM4D(Gi)-mCherry | Addgene | 44362 |
| AAV5-EF1a-fDIO-HA-NLS-Cre | This paper; available at Addgene | N/A |
| AAV9-CAG-FLEX-EGFP; AI854 | Penn Vector Core | N/A |
| AAV9-DIO-SynP-mCherry | MGH Vector Core | AAV-RN1 |
| AAV9-DIO-SynP-EYFP | MGH Vector Core | AAV-RN2 |
| PRV-lp298 (PRV-mCherry) | This paper | N/A |
| PRV-152 (PRV-GFP) | Smith et al., 2000 | N/A |
| Software and Algorithms | ||
| GraphPad Prism 6.0 | GraphPad | N/A |
| ClearMap | Renier et al., 2016 | N/A |
| Imaris 9.0.1 | Bitplane | N/A |
| Terastitcher | University Campus Bio-Medico of Rome | N/A |