Prebiotic fermentation in the gut often leads to the coproduction of short-chain fatty acids (SCFAs) and gases. While excess gas production can be a potential problem for those with functional gut disorders, gas production is rarely considered during prebiotic design. In this study, we combined the use of theoretical models and an ex vivo experimental platform to illustrate that both the chemical composition of the prebiotic and the community composition of the human gut microbiota can affect the volume and content of gas production during prebiotic fermentation. Specifically, more prevalent metabolic processes such as hydrogen production were strongly affected by the oxidation state of the probiotic, while rare metabolisms such as methane production were less affected by the chemical nature of the substrate and entirely dependent on the presence of Methanobacteria in the microbiota.
KEYWORDS: functional heterogeneity, gut microbiome, intestinal gas, prebiotics
ABSTRACT
Prebiotics confer benefits to human health, often by promoting the growth of gut bacteria that produce metabolites valuable to the human body, such as short-chain fatty acids (SCFAs). While prebiotic selection has strongly focused on maximizing the production of SCFAs, less attention has been paid to gases, a by-product of SCFA production that also has physiological effects on the human body. Here, we investigate how the content and volume of gas production by human gut microbiota are affected by the chemical composition of the prebiotic and the community composition of the microbiota. We first constructed a linear system model based on mass and electron balance and compared the theoretical product ranges of two prebiotics, inulin and pectin. Modeling shows that pectin is more restricted in product space, with less potential for H2 but more potential for CO2 production. An ex vivo experimental system showed pectin degradation produced significantly less H2 than inulin, but CO2 production fell outside the theoretical product range, suggesting fermentation of fecal debris. Microbial community composition also impacted results: methane production was dependent on the presence of Methanobacteria, while interindividual differences in H2 production during inulin degradation were driven by a Lachnospiraceae taxon. Overall, these results suggest that both the chemistry of the prebiotic and the composition of the microbiota are relevant to gas production. Metabolic processes that are relatively prevalent in the microbiome, such as H2 production, will depend more on substrate, while rare metabolisms such as methanogenesis depend more strongly on microbiome composition.
INTRODUCTION
The gut microbiota plays an important role in human nutrition and health, leading to increasing interest in modulation of the gut microbiome via dietary interventions for improving human health (1–3). Compounds that can be selectively metabolized by microbes in the gut resulting in beneficial effects on the host are defined as prebiotics (4). While some phenolic compounds and fatty acids are suspected to have prebiotic activities, most known prebiotics are dietary carbohydrates that are neither digested nor absorbed in the human small intestine and are thus capable of reaching the colon and promoting the growth of selective beneficial bacteria (4, 5). These bacteria, in turn, can prevent the colonization of pathogens or produce metabolites that are beneficial for the human body, most notably short-chain fatty acids (SCFAs) such as acetate, propionate, and butyrate. These SCFAs not only contribute directly to host energy metabolism but have a number of positive effects on host physiology. Butyrate is the major energy source for colonocytes and enterocytes (6) and can also activate gluconeogenesis and modulate inflammatory responses and cytokine levels via G protein‐coupled receptors or histone deacetylases (7). Similarly, acetate and propionate are involved in the regulation of host immune or metabolic systems (7, 8). Thus, selection for prebiotics has largely focused on those that allow the proliferation of bacteria that maximize production of SCFAs (5, 9, 10).
SCFA fermentation from carbohydrates by the gut microbiota is often coupled with the production of gases. Production of H2 is often necessary for the cycling of NAD+/NADH during fermentation, and CO2 is released whenever decarboxylation occurs (11). H2 can be further utilized by methanogens and sulfate reducers for the production of CH4 and H2S (12). Most intestinal gas is absorbed into the bloodstream and removed via the lungs (13), but it can still have physiological effects on the human body. The volume of gas production can affect the distension of the colonic wall and in turn affect the speed of material transition through the colon (14). Methane production can result in slowed intestinal transit and reduced serotonin levels in the gastrointestinal tract, potentially impacting constipation-predominant irritable bowel syndrome (IBS-C) and chronic constipation (15). Therefore, gas production may be an important factor to consider in the selection of prebiotics, especially since bloating is a major symptom for many functional gut disorders such as IBS (16).
Many prebiotics are already known to impact fermentation products. For example, short-chain fructooligosaccharides (FOS) and inulin are some of the most extensively documented prebiotics, because they promote the growth of bifidobacteria and increase SCFA production (4, 5). However, the low-FODMAP (fermentable oligosaccharide, disaccharide, monosaccharide, and polyol) diet has been shown to improve IBS symptoms in some patients, because foods containing FOS and inulin can increase luminal distension and gas production (17, 18). Thus, it may be valuable to identify prebiotics that maximize SCFA and minimize gas production or minimize the production of specific gases. However, few studies that consider the efficacy of prebiotics simultaneously take gas and SCFA production into account, and systematic investigations on factors that affect gas production in prebiotic fermentation are lacking.
In this study, we investigated whether the chemical composition of the prebiotic and heterogeneity in the compositions of gut microbiota can affect the content and volume of gas production during prebiotic fermentation. We compared the fermentation products of two common prebiotics, inulin and pectin, both theoretically via linear system modeling and experimentally via an ex vivo framework that measures gas and SCFA production of stool microbiota responding to fiber addition (19). We find that inulin, a more reduced carbohydrate, produces more H2 than pectin, but the amount of H2 production is strongly associated with a Lachnospiraceae amplicon sequencing variant (ASV). Inulin also yielded greater amounts of the more reduced SCFA butyrate and less acetate. Methane production is, however, less affected by the chemical nature of the substrate, being entirely dependent on the level of Methanobacteria in the microbiota. Overall, these results suggest that the production of different gases upon prebiotic fermentation by gut microbiota are differentially affected by the chemical nature of the prebiotic and microbiome compositions.
RESULTS
Modeling community production with mass and electron balance.
To explore the general effect of prebiotic chemical composition on fermentation product formation, we established a linear system model that allowed us to determine the theoretical range of product output considering mass and electron balance. Considering a system of n chemicals as possible inputs and outputs, made up of a total of m chemical elements, we defined a matrix M in which the rows represent different elements and columns represent different chemicals; the elemental composition of a chemical is thus a column in M. The total number of valence electrons in the chemical is also counted as an “element” and consists of a separate row in M. Thus, any reaction that satisfies both mass and electron balance is an n-dimensional vector s, whose elements are the stoichiometric coefficients of the chemicals in M and satisfy Ms = 0 (Fig. 1a). By definition, s must be within the null space of M. The feasible product space of the biological system, represented by the elements in s that are coefficients of the possible products, is thus a convex cone defined by the linear combinations of the basis vector of null(M). Since our model did not account for the thermodynamic constraints on the metabolic fluxes within the system, it represented an upper limit of the feasible product space.
Feasible product space of pectin fermentation is more limited than that of inulin.
We applied our model to compare the feasible product space for the fermentation of 1 mol of inulin (C6nH10n+2O5n+1) to that of 1 mol of pectin (C6nH8n+2O6n+1) in a closed system. Since we were modeling product output from carbohydrate input, we only included C, H, O, and valence electrons as rows in our matrix M. For products (columns) in M, we included the three most abundant SCFAs in the gut (acetate, propionate, and butyrate) and the three major components of intestinal gas (H2, CH4, and CO2) as well as water and biomass (represented by CH1.8O0.5N0.2, the mean chemical formula for microbial biomass [20]) (Fig. 1). All product concentrations were restricted to be nonnegative to simulate a closed system (i.e., product formation is solely from fiber input). Our model showed that in a closed system, the product space of pectin was more restricted than that of inulin (see Fig. S1 in the supplemental material); in particular, inulin had more potential for H2 production, while pectin had more potential for the production of CO2 (Fig. 1c). Model predictions were conserved even if further constraints were placed on the system, i.e., 15% of C in the fiber is converted into biomass as in a typical carbohydrate fermentation (Fig. S2a, S2b, and S2c) (21).
Pectin degradation takes up reducing agents from the environment.
We next asked if our theoretical predictions could be experimentally validated using an ex vivo framework in which we measured the response of stool microbiota to fiber addition. First, stools from 9 healthy human subjects were each homogenized with phosphate-buffered saline (PBS) under anaerobic conditions to create fecal slurries. The slurries were then incubated in serum bottles at 37°C starting with 100% N2 in the headspace, with inulin, pectin, cellulose, or no additional fiber input (Fig. 2a). Since our preliminary testing showed that degradation of soluble fibers in this system was almost entirely complete within 24 h (19), we used the gas and SCFA concentrations at 24 h as the experimental product concentrations for comparison to those predicted from our theoretical models. Because the fecal slurry itself contained a certain amount of residue material from food digestion in the human body, even samples that did not receive additional fiber produced gas and SCFAs; the fermentation products of a certain fiber in a sample were thus determined as the difference between product concentrations measured in a sample which received additional fiber and those in a sample which did not. We found the gas and SCFA concentrations of samples with cellulose was not significantly different from the control samples, likely because cellulose was not soluble in water and precipitated as a thick white layer at the bottom of the serum bottles. We thus focused our analyses on samples with soluble fiber input, i.e., inulin and pectin.
Focusing on gas production in the ex vivo systems, we found that the amount of H2 produced by pectin fermentation was significantly lower than that of inulin (Fig. 2b) (Kruskal-Wallis test, paired, P = 0.004), and the total amount of gas production was also lower (Kruskal-Wallis test, paired, P = 0.07). However, we did not observe a larger amount of CO2 production in pectin fermentation than in inulin fermentation as theoretically predicted; in fact, the measured CO2 productions from pectin fermentation did not fall within the previous theoretically predicted range (Fig. 3a). This was also the case for acetate production in some samples that fermented inulin. Since our model only considers the most basic laws of chemistry and represents the maximum possible theoretical product range for a closed system, we hypothesized that the experimental violation of the results from the theoretical model was due to assuming that our experimental system was closed. Indeed, despite the serum bottle being a closed system with no material exchange with the environment outside the bottle, fermentation of the additional fiber should be seen as a subsystem that can exchange products with the other subsystem in the bottle that ferments residue material in the fecal slurry (Fig. 3b).
We thus investigated what input the “fiber subsystem” would need from the “residue subsystem” for the measured CO2 to fall within the feasible product range determined by the theoretical model. Since, on average, the samples that did not receive additional fiber produced approximately one-third as much gas and SCFAs as those that did (see Fig. S4), we limited the input from the residue subsystem to the equivalent amount of product that can be produced by 1/3 mol of inulin or pectin. Allowing one input at a time, we found that only when H2 or CH4 was used as input would the measured CO2 fall within the predicted range (Fig. 3c). Although we did not observe net uptake of either H2 or CH4 in our experimental data, but because both H2 and CH4 are chemicals with reducing power, there was likely influx of other reducing substrates not presented in our model from the “residue subsystem” to the “fiber subsystem.” Thus, in our ex vivo system, pectin degradation not only had a lower net production of H2 than that of inulin but also took up reducing agents from the surrounding environment. We thus speculate that when pectin is degraded in the human gut, it is also taking up reducing agents—a process for which consequences are unclear and possibly worth further investigation.
H2, acetate, and butyrate distinguish the product profile of inulin and pectin degradation.
We next asked if the overall product profiles of inulin degradation and pectin degradation can be distinguished from each other and whether changing the fiber or microbial community contributed more to the variation in product profiles. We found that product profiles primarily clustered by fiber and secondarily by human subjects (Fig. 4) (permutational multivariate analysis of variance [PERMANOVA], R2(fiber) = 0.79, P(fiber) = 0.001; R2(subjects) = 0.12, P(subjects) = 0.02). Given that inulin fermentation generated significantly more H2 and butyrate but less acetate that that of pectin (Fig. 2b), we hypothesized these are the three major products that would allow the product profiles of inulin and pectin fermentations to be distinguished. Indeed, the top products found to separate the inulin and pectin samples in the PERMANOVA were acetate, butyrate, and H2. Importantly, the coefficients for the more reduced products, H2 and butyrate, were in the opposite direction of acetate (see Fig. S3). The more oxidized substrate, pectin, produced more of the most oxidized SCFA, acetate, while inulin produced more H2 and the most reduced SCFA, butyrate (Fig. 2b).
Relative effects of microbiome and substrate chemistry on gas production differ among gases.
We further explored if there were signatures within the microbiomes that promoted the production of gases. Since levels of H2 production were generally low for pectin fermentation, we investigated if there were specific ASVs associated with net H2 production during inulin fermentation. Selecting for these ASVs via Lasso regression identified a Lachnospiraceae amplicon sequencing variant (ASV) positively associated with net H2 production (Fig. 5a) (Pearson’s r = 0.97, P = 1.14 × 10−5) (see Fig. S5). Since net H2 production in the gut is the difference between the total production of H2 and the total consumption of H2 (12), and Lachnospiraceae can be either hydrogen producers or consumers (22, 23), the positive association of the Lachnospiraceae ASV with H2 production in the human gut may indicate that net H2 production is more dependent on H2 production than on consumption. Consistent with this hypothesis, H2 consumption abilities of gut microbiota may be more consistent between different people than H2 production because of the higher diversity of H2 consumption pathways (methanogenesis, reductive acetogenesis, and sulfate reduction) than those for production. It was, however, observed that net H2 production was lowest in the samples that produced methane, probably because the amount of sulfate in the ex vivo system is not enough to for the most energetically favorable H2 consumption pathway, sulfate reduction, to consume all the H2 produced (see Text S1), and methanogenesis is more energetically favorable than reductive acetogenesis (24).
In contrast to H2, whose production was strongly affected by substrate, we found that methane production was solely dependent on whether there were detectable levels of Methanobacteria in the microbiota (Fig. 5b). Given that methane is a downstream product of H2 (Fig. 5c), we asked why methane production was not affected by substrate chemistry but H2 production was. We hypothesized that this is because there are generally large amounts of bacteria in the human gut that contain carbohydrate-active enzymes and hydrogenases that allow fiber breakdown and hydrogen production; thus, the amounts of these enzymes are not a limiting factor, allowing hydrogen production to be instead dependent on substrate stoichiometry. Meanwhile, a large portion of the human population does not harbor sufficient numbers of methanogens, making them the limiting factor for methane production; however, when the number of methanogens is sufficient, they are not limited by the amount of H2 production because methanogens are stronger competitors for H2 than reductive acetogens. To test this hypothesis, we surveyed the abundances of carbohydrate-active enzymes (CAZymes) for inulin and pectin as well as hydrogenogenic hydrogenases and methyl coenzyme M (methyl-CoM) reductases (mcrA, marker gene for methanogens) in the metagenomes of 160 randomly selected healthy human subjects from the human microbiome project (HMP). While only approximately 20% of subjects had detectable levels of methanogens, nearly all subjects harbored CAZymes for inulin and pectin degradation as well as hydrogenogenic hydrogenases (Fig. 5d). The percentage of subjects (20%) with detectable methanogens in the HMP data is in accordance with our results: 2 of the 9 subjects were methane producers in our ex vivo experiment. Thus, overall, the production of more-“general” metabolites such as H2 is more likely to be affected by the chemical composition of the prebiotic, while more-“rare” metabolites such as methane are more likely to be limited by the organisms that produce it.
DISCUSSION
In this study, we used a combination of theoretical models and an ex vivo experimental framework to examine how the chemistry of prebiotics and the composition of the gut microbiota influence gas production during prebiotic fermentation by gut microbiota. Specifically selecting two different common prebiotics (inulin and pectin) with different levels of oxidation, we find that metabolites that can be produced by more organisms in the human gut, such as H2, are more affected by the chemical composition of prebiotics than metabolites that are produced by less common organisms in the gut, such as methane. Overall, these results suggest that both the chemical nature of the prebiotic and the individual’s gut microbiome needs to be considered when administering prebiotics to individuals.
Our data also reveal that there may be general trade-offs in the production of SCFAs versus that of gas. For example, while inulin fermentation leads to more production of the more reduced SCFA butyrate, it also leads to more production of the reducing agent H2, in turn, increasing overall gas production. However, which is more preferable for the subject—more production of butyrate, less production of overall gas, or just less production of H2—is often unknown and specific to the individual subject. This can be further complicated if interindividual differences in H2 and SCFA production are considered: not every individual produces more butyrate when fermenting inulin. Similarly, for pectin, we were able to infer by comparing the experimental data to the theoretical product range that pectin degradation requires uptake of reducing agents from the surrounding environment. Again, what effect this has on the host is unknown: would the uptake of these reducing agents lead to the generation of more reactive oxygen species that can directly attack cells in the gut epithelial barrier, interfere with iron uptake, or initiate lipid peroxidation processes (25)? Would this be costlier to the host than generating more H2? More importantly, we also lack a way to evaluate if the scale of the differences is large enough for them to count as a factor in prebiotic selection.
These problems emphasize that the effect of prebiotics on gut and human health must be looked at from both individual and systems perspectives. Often, a compound is deemed as a prebiotic because it can increase the growth of known beneficial microbes such as bifidobacteria and lactobacilli or promote the production of target metabolites. However, the full diversity of a mixed-culture environment such as the human gut must be considered when selecting for prebiotics: it is very hard to only selectively grow organisms that produce one or a few metabolites of interest, and the effect of any by-products must be considered. Interindividual differences in product formation due to heterogeneity in gut microbiota composition, as well as responses to the metabolites produced, must also be considered. Our use of theoretical modeling and the ex vivo experimental system to explore gas production and its relationship to SCFA production is just a beginning: these are relatively inexpensive and simple methods to shine light on important points that should be considered in prebiotic design.
The ex vivo experimental system can be seen as where techniques originating from many fields come together in a compromised way. Compared to the breath test often used in clinical practice for diagnosis of lactose intolerance or small intestinal bacterial overgrowth (26), it fails to measure gas production in situ; also, compared to cultures with known bacterial content and medium composition, it is more challenging to obtain measurements that can directly match the results of theoretical models. The fact that some of our experimental measurements do not fall within the predicted theoretical range reflects both the strength and weaknesses of our system: while our system is not perfect for testing hypotheses, it is a good system for pointing at possible directions and generating hypotheses that could be further tested. An important future improvement to the system would be better separation of the gut microbial community with the fecal matter from which it originates so that the experimental system truly mimics the “closed system” as described in the theoretical model. Specifically, a known reductive substrate could be added to the system to quantitatively measure the uptake of reducing agents during the degradation of different fibers.
Overall, our study is a first step toward developing a system where the unique microbiome composition of each individual can be measured simultaneously with its fermentation products for different kinds of fibers. In the future, a more systematic evaluation on what important factors other than the formation of beneficial metabolites should be considered in prebiotic design is needed.
MATERIALS AND METHODS
Experimental model and participant details.
Nine healthy human volunteers were enrolled into the study under the supervision of the MIT Committee on the Use of Humans as Experimental Subjects (COUHES), who approved the study under protocol number 1510271631. All participants provided written informed consent, and the study was conducted in accordance with the relevant guidelines and regulations. To be included, participants had to be between 18 and 70 years of age, have a body mass index (BMI) between 18 and 30, and not have a history of inflammatory bowel diseases/syndrome, type-2 diabetes, kidney diseases, intestinal obstruction, or colorectal cancer. They were also not currently pregnant or breastfeeding and had not received antibiotic treatment in the 6 months leading up to the study. Enrollment occurred between June 2017 and Oct 2017. The study group included 4 female and 5 male individuals, all between 25 and 40 years of age.
Linear system model for modeling community production.
The product space for the system Ms = 0 is a polytope defined by linear combinations of the basis vectors of null(M), i.e., Bx = s. Constraints on the product space (i.e., for the closed system, all elements in s corresponding to products are nonnegative) were used to find the vertices of the polytope of x by converting the half-space representation (the intersection of half spaces, represented by Bx = s) into vertex representation (set of extreme points of the polytope). Vertices of the polytope of s were calculated by multiplying B with the vertices of polytope x. The vertices for s were used to draw two-dimensional (2D) hulls for pairs of products to visualize the product polytope, as in Fig. 1c and 3a and c. When product input was allowed for the system, the constraint on the element in s corresponding to the input product was relaxed to be larger than the negative of the equivalent amount of product that can be produced by 1/3 mol of inulin or pectin (on average, control samples produced approximately one-third as much SCFA and/or gas than samples with inulin or pectin treatment) (see Fig. S4 in the supplemental material).
The basis set of vectors for the null space of matrix M was calculated from the QR-decomposition of the matrix using the R package “pracma” (27). The conversions of half-space representation to vertex representation of polytopes were performed using the R package “rcdd” (28).
Setup of ex vivo system.
The setup of the ex vivo system was the same as that by Gurry et al. (19), with some adaptation for gas measurements. Briefly, fresh stool samples were collected and homogenized with reduced PBS containing 0.1% l-cysteine at a ratio of 1 g/5 ml. Fiber was spiked in to the homogenates from stock solutions such that the final concentrations of fibers in the samples were as follows: control (no fiber), 10 g/liter inulin, 5 g/liter pectin, or 20 g/liter cellulose. The concentration of the fibers spiked in were in accordance with the highest daily amount of the certain fiber that a healthy subject could ingest without experiencing discomfort determined in a previous in vivo dietary study (29) as well as with the relative solubility of the fibers (more total input if the fiber was less soluble). For each participant, 2 ml of the final fecal slurry of each condition was added in triplicates to 60-ml glass serum bottles (Supelco, Bellefonte, PA). The serum bottles containing the samples were transferred to a vinyl anaerobic chamber filled with 100% N2, with no detectable amounts of CO2 and H2, and sealed in the chamber using magnetic crimp seals with polytetrafluoroethylene (PTFE)-silicone septa (Supelco, Bellefonte, PA). A total of 12 bottles per participant were incubated at 37°C for 24 h with no shaking.
Gas and SCFA measurements.
Concentrations of headspace gases were determined using gas chromatography. We used a Shimadzu GC-2014 gas chromatograph (GC) configured with a packed column (Carboxen-1000, 5 ft by 1/8 in. [Supelco, Bellefonte, PA]) held at 140°C, argon carrier gas, and thermal conductivity (TCD) and methanizer-flame ionization (FID) detectors. At the end of the 24-h incubation period, subsamples of the headspace (0.20 cm3 at the laboratory temperature, ca. 23°C) from each serum bottle were taken via a gas-tight syringe and injected into the column. Gas concentrations were determined by comparing the partial pressures of samples with standards of known concentrations. The accuracy of the analyses, evaluated from standards, was ±5%. Measurements of H2 were taken using the TCD while measurements of CH4 and CO2 were taken with the FID.
SCFA measurements were made from taking 1 ml of fecal slurry from each serum bottle immediately after the GC measurements were taken and freezing the fecal slurry at −80°C until time of measurement. SCFA measurements were made on an Agilent 7890B system with an FID at the Harvard Digestive Disease Core (Agilent Technologies, Santa Clara, CA). Detailed procedures for SCFA measurements are the same as those described by Gurry et al. (19). Although the amounts of 10 volatile acids (including acetic, propionic, isobutyric, butyric, isovaleric, valeric, isocaproic, caproic, and heptanoic acids) were reported, all but the acetic, butyric, and propionic acids were in trace amounts, and we only used these three SCFAs for our models.
Machine learning and statistics.
The principlal-coordinate analyses of the ex vivo fermentation products were performed with the R package “ape” using Euclidian distance matrices (30). The alpha and beta diversities of the microbial community compositions were calculated with the R package “vegan.” PERMANOVAs were performed with the function adonis in the R package vegan (31), with 999 permutations. The Lasso regression was performed with the R package “glmnet” (32), and cross-validation was performed with a leave-one-out approach.
DNA extraction, library preparation, and sequencing.
The Mo Bio PowerSoil-htp 96 kit (now Qiagen catalog number 12955-4), with minor modifications, was used to extract the DNA from all fecal samples. For all samples, 250 μl of the fecal slurry was used with the Mo Bio High Throughput PowerSoil bead plate (12955-4 BP). 16S rRNA gene amplicon libraries (V4 hypervariable region, U515 to E786) using a two-step PCR approach were prepared according to the method described by Preheim et al. (33). Samples were sequenced on an Illumina MiSeq (paired-end [PE] 150 + 150) at the Broad Walk-Up Sequencing platform (Broad Institute, Cambridge, MA). The average sequencing depth of the samples was 53,046 reads/sample.
16S rRNA amplicon data analysis.
All 16S rRNA amplicon libraries were processed according to a custom pipeline, where cutadapt (34) was used for primer trimming, QIIME 1.9 (35) was used for demultiplexing, and DADA2 (36) was used to infer amplicon sequence variants (ASVs). Default settings were used except that only the forward reads were used due to issues with merging reads, and the forward reads were truncated to 110 bp. Taxonomy for the sequence variants was assigned using the RDP database (37). The alpha and beta diversities of all communities are shown in Fig. S6a and b in the supplemental material.
Metagenome analysis.
We downloaded 160 randomly selected metagenomes from the human stool microbial communities of the Human Microbiome Project (National Institutes of Health, USA). Each metagenome was rarefactioned to 20 million reads (forward plus reverse) using seqtk seeded with the parameter –s100. The rarefactioned metagenomes were screened in DIAMOND (maximum number of high scoring pairs [HSPs] per subject sequence to save for each query = 1, blastx) against hydrogenogenic hydrogenases retrieved from the HydDB database (38), mcrA genes retrieved from the PhyMet database (39), and CAZymes from the dbCAN database (40). Results were then filtered (length of amino acid, >25 residues; percent identical matches, >65% [mcrA and hydrogenases] or >35% [CAZymes]). Reads were eventually normalized to reads per kilobase million using the formula .
Data availability.
All amplicon sequencing data generated in this study can be accessed on the US National Center for Biotechnology Information SRA database under BioProject PRJNA587309. All gas and SCFA measurements, ASV tables, and code for data analysis are available at https://github.com/cusoiv.
ACKNOWLEDGMENTS
This work was supported by a grant from the Rasmussen Family Foundation.
We thank the Ono Lab at MIT and Jeemin Rhim for teaching and allowing us to use their GC for measurements of gas production, and David Sontag at the MIT Computer Science and Artificial Intelligence Laboratory for consultation with the linear system model. We thank the Broad Walk-Up Sequencing center for their assistance with sequencing and Harvard Digestive Disease Core for their assistance on GC for measurements of SCFA production. We also wish to thank Chengzhen Dai, Siavash Isazadeh, Chuliang Song, Fangqiong Ling, and Martin Polz for helpful discussions and/or comments on various versions of the manuscript.
E.J.A. is a cofounder and shareholder of Finch Therapeutics, a company that specializes in microbiome-targeted therapeutics.
Footnotes
Citation Yu X, Gurry T, Nguyen LTT, Richardson HS, Alm EJ. 2020. Prebiotics and community composition influence gas production of the human gut microbiota. mBio 11:e00217-20. https://doi.org/10.1128/mBio.00217-20.
REFERENCES
- 1.Cotillard A, Kennedy SP, Kong LC, Prifti E, Pons N, Le Chatelier E, Almeida M, Quinquis B, Levenez F, Galleron N, Gougis S, Rizkalla S, Batto J-M, Renault P, ANR MicroObes Consortium, Doré J, Zucker J-D, Clément K, Ehrlich SD. 2013. Dietary intervention impact on gut microbial gene richness. Nature 500:585–588. doi: 10.1038/nature12480. [DOI] [PubMed] [Google Scholar]
- 2.Tremaroli V, Bäckhed F. 2012. Functional interactions between the gut microbiota and host metabolism. Nature 489:242–249. doi: 10.1038/nature11552. [DOI] [PubMed] [Google Scholar]
- 3.De Filippis F, Vitaglione P, Cuomo R, Berni Canani R, Ercolini D. 2018. Dietary interventions to modulate the gut microbiome—how far away are we from precision medicine. Inflamm Bowel Dis 24:2142–2154. doi: 10.1093/ibd/izy080. [DOI] [PubMed] [Google Scholar]
- 4.Gibson GR, Hutkins R, Sanders ME, Prescott SL, Reimer RA, Salminen SJ, Scott K, Stanton C, Swanson KS, Cani PD, Verbeke K, Reid G. 2017. Expert consensus document: the International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of prebiotics. Nat Rev Gastroenterol Hepatol 14:491–502. doi: 10.1038/nrgastro.2017.75. [DOI] [PubMed] [Google Scholar]
- 5.Holscher HD. 2017. Dietary fiber and prebiotics and the gastrointestinal microbiota. Gut Microbes 8:172–184. doi: 10.1080/19490976.2017.1290756. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 6.Canani RB, Costanzo MD, Leone L, Pedata M, Meli R, Calignano A. 2011. Potential beneficial effects of butyrate in intestinal and extraintestinal diseases. World J Gastroenterol 17:1519–1528. doi: 10.3748/wjg.v17.i12.1519. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 7.Belcheva A, Irrazabal T, Martin A. 2015. Gut microbial metabolism and colon cancer: can manipulations of the microbiota be useful in the management of gastrointestinal health? Bioessays 37:403–412. doi: 10.1002/bies.201400204. [DOI] [PubMed] [Google Scholar]
- 8.Shibata N, Kunisawa J, Kiyono H. 2017. Dietary and microbial metabolites in the regulation of host immunity. Front Microbiol 8:2171. doi: 10.3389/fmicb.2017.02171. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 9.Míguez B, Gómez B, Gullón P, Gullón B, Alonso JL. 2016. Pectic oligosaccharides and other emerging prebiotics In Rao V, Rao LG. (ed), Probiotics and prebiotics in human nutrition and health. InTech, Rijeka, Croatia. [Google Scholar]
- 10.Verspreet J, Damen B, Broekaert WF, Verbeke K, Delcour JA, Courtin CM. 2016. A critical look at prebiotics within the dietary fiber concept. Annu Rev Food Sci Technol 7:167–190. doi: 10.1146/annurev-food-081315-032749. [DOI] [PubMed] [Google Scholar]
- 11.Müller V. 2008. Bacterial fermentation. John Wiley & Sons, Hoboken, NJ. [Google Scholar]
- 12.Carbonero F, Benefiel AC, Gaskins HR. 2012. Contributions of the microbial hydrogen economy to colonic homeostasis. Nat Rev Gastroenterol Hepatol 9:504–518. doi: 10.1038/nrgastro.2012.85. [DOI] [PubMed] [Google Scholar]
- 13.Cormier RE. 1990. Abdominal Gas In Walker HK, Hall WD, Hurst JW (ed), Clinical methods: the history, physical, and laboratory examinations, 3rd ed Butterworths, Boston, MA. [PubMed] [Google Scholar]
- 14.Azpiroz F. 2005. Intestinal gas dynamics: mechanisms and clinical relevance. Gut 54:893–895. doi: 10.1136/gut.2004.048868. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 15.Sahakian AB, Jee S-R, Pimentel M. 2010. Methane and the gastrointestinal tract. Dig Dis Sci 55:2135–2143. doi: 10.1007/s10620-009-1012-0. [DOI] [PubMed] [Google Scholar]
- 16.Seo AY, Kim N, Oh DH. 2013. Abdominal bloating: pathophysiology and treatment. J Neurogastroenterol Motil 19:433–453. doi: 10.5056/jnm.2013.19.4.433. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 17.McIntosh K, Reed DE, Schneider T, Dang F, Keshteli AH, De Palma G, Madsen K, Bercik P, Vanner S. 2017. FODMAPs alter symptoms and the metabolome of patients with IBS: a randomised controlled trial. Gut 66:1241–1251. doi: 10.1136/gutjnl-2015-311339. [DOI] [PubMed] [Google Scholar]
- 18.Gibson PR, Shepherd SJ. 2010. Evidence-based dietary management of functional gastrointestinal symptoms: the FODMAP approach. J Gastroenterol Hepatol 25:252–258. doi: 10.1111/j.1440-1746.2009.06149.x. [DOI] [PubMed] [Google Scholar]
- 19.Gurry T, Nguyen LTT, Yu X, Alm EJ. 24 January 2020. Functional heterogeneity in the fermentation capabilities of the healthy human gut microbiota. bioRxiv doi: 10.1101/2020.01.17.910638. [DOI] [PMC free article] [PubMed]
- 20.Roels JA. 1983. Energetics and kinetics in biotechnology. Elsevier Biomedical Press, New York, NY. [Google Scholar]
- 21.Sawyer CN, McCarty PL, Parkin GF. 2003. Chemistry for environmental engineering and science. McGraw-Hill Education, New York, NY. [Google Scholar]
- 22.Gagen EJ, Padmanabha J, Denman SE, McSweeney CS. 2015. Hydrogenotrophic culture enrichment reveals rumen Lachnospiraceae and Ruminococcaceae acetogens and hydrogen-responsive Bacteroidetes from pasture-fed cattle. FEMS Microbiol Lett 362:fnv104. doi: 10.1093/femsle/fnv104. [DOI] [PubMed] [Google Scholar]
- 23.Rajilić-Stojanović M, de Vos WM. 2014. The first 1000 cultured species of the human gastrointestinal microbiota. FEMS Microbiol Rev 38:996–1047. doi: 10.1111/1574-6976.12075. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 24.Conrad R, Wetter B. 1990. Influence of temperature on energetics of hydrogen metabolism in homoacetogenic, methanogenic, and other anaerobic bacteria. Arch Microbiol 155:94–98. doi: 10.1007/BF00291281. [DOI] [Google Scholar]
- 25.Owen RW, Spiegelhalder B, Bartsch H. 2000. Generation of reactive oxygen species by the faecal matrix. Gut 46:225–232. doi: 10.1136/gut.46.2.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 26.Saad RJ, Chey WD. 2007. Breath tests for gastrointestinal disease: the real deal or just a lot of hot air? Gastroenterology 133:1763–1766. doi: 10.1053/j.gastro.2007.10.059. [DOI] [PubMed] [Google Scholar]
- 27.Borchers HW. 2019. pracma: practical numerical math functions. https://rdrr.io/rforge/pracma/.
- 28.Geyer CJ, Meeden GD. 2019. rcdd: computational geometry. https://rdrr.io/cran/rcdd/.
- 29.Gurry T, HST Microbiome Consortium*, Gibbons SM, Nguyen LTT, Kearney SM, Ananthakrishnan A, Jiang X, Duvallet C, Kassam Z, Alm EJ. 2018. Predictability and persistence of prebiotic dietary supplementation in a healthy human cohort. Sci Rep 8:12699. doi: 10.1038/s41598-018-30783-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 30.Paradis E, Schliep K. 2019. ape 5.0: an environment for modern phylogenetics and evolutionary analyses in R. Bioinformatics 35:526–528. doi: 10.1093/bioinformatics/bty633. [DOI] [PubMed] [Google Scholar]
- 31.Oksanen J, Blanchet FG, Kindt R, Legendre P, Minchin P, O’Hara R, Simpson G, Solymos P, Stevens M, Wagner H. 2013. Vegan: community ecology package. R package version. 2.0–10. R Foundation for Statistical Computing, Vienna, Austria. [Google Scholar]
- 32.Friedman J, Hastie T, Tibshirani R. 2010. Regularization paths for generalized linear models via coordinate descent. J Stat Softw 33:1–22. [PMC free article] [PubMed] [Google Scholar]
- 33.Preheim SP, Perrotta AR, Martin-Platero AM, Gupta A, Alm EJ. 2013. Distribution-based clustering: using ecology to refine the operational taxonomic unit. Appl Environ Microbiol 79:6593–6603. doi: 10.1128/AEM.00342-13. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 34.Martin M. 2011. Cutadapt removes adapter sequences from high-throughput sequencing reads. Embnet J 17:10–12. doi: 10.14806/ej.17.1.200. [DOI] [Google Scholar]
- 35.Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, Fierer N, Peña AG, Goodrich JK, Gordon JI, Huttley GA, Kelley ST, Knights D, Koenig JE, Ley RE, Lozupone CA, McDonald D, Muegge BD, Pirrung M, Reeder J, Sevinsky JR, Turnbaugh PJ, Walters WA, Widmann J, Yatsunenko T, Zaneveld J, Knight R. 2010. QIIME allows analysis of high-throughput community sequencing data. Nat Methods 7:335–336. doi: 10.1038/nmeth.f.303. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 36.Callahan BJ, McMurdie PJ, Rosen MJ, Han AW, Johnson AJA, Holmes SP. 2016. DADA2: high-resolution sample inference from Illumina amplicon data. Nat Methods 13:581–583. doi: 10.1038/nmeth.3869. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 37.Cole JR, Wang Q, Fish JA, Chai B, McGarrell DM, Sun Y, Brown CT, Porras-Alfaro A, Kuske CR, Tiedje JM. 2014. Ribosomal Database Project: data and tools for high throughput rRNA analysis. Nucleic Acids Res 42:D633–D642. doi: 10.1093/nar/gkt1244. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 38.Søndergaard D, Pedersen CNS, Greening C. 2016. HydDB: a web tool for hydrogenase classification and analysis. Sci Rep 6:34212. doi: 10.1038/srep34212. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 39.Michał B, Gagat P, Jabłoński S, Chilimoniuk J, Gaworski M, Mackiewicz P, Marcin Ł. 2018. PhyMet2: a database and toolkit for phylogenetic and metabolic analyses of methanogens. Environ Microbiol Rep 10:378–382. doi: 10.1111/1758-2229.12648. [DOI] [PubMed] [Google Scholar]
- 40.Zhang H, Yohe T, Huang L, Entwistle S, Wu P, Yang Z, Busk PK, Xu Y, Yin Y. 2018. dbCAN2: a meta server for automated carbohydrate-active enzyme annotation. Nucleic Acids Res 46:W95–W101. doi: 10.1093/nar/gky418. [DOI] [PMC free article] [PubMed] [Google Scholar]
Associated Data
This section collects any data citations, data availability statements, or supplementary materials included in this article.
Supplementary Materials
Data Availability Statement
All amplicon sequencing data generated in this study can be accessed on the US National Center for Biotechnology Information SRA database under BioProject PRJNA587309. All gas and SCFA measurements, ASV tables, and code for data analysis are available at https://github.com/cusoiv.