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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Bone. 2020 Jul 3;139:115521. doi: 10.1016/j.bone.2020.115521

Figure 3.

Figure 3.

Lineage tracing studies of Prx1-expressing cells in intact tibias.

(A) Schematic representation of 4OH-TAM injections in postnatal development studies in Prx1-CreER-GFP-ROSA-LacZ mice. (B) Representative histological LacZ/Safranin O staining of early postnatal study. For all pictures, periosteal surface is facing the right side of the images. (C) Representative histological LacZ/Safranin O staining of late postnatal study. For all pictures, periosteal surface is facing the top of the images. (D) Representative IF images in periosteum of Prx1-CreER-GFP-ROSA-LacZ mice, immunostained respectively with β-Gal (green) and Ki67 (red) antibodies, counterstained with DAPI (blue). (E) Schematic representation of slow-proliferating study in Prx1-CreER-GFP-ROSA-LacZ mice. (F) Representative IF images in Prx1-CreER-GFP-ROA-LacZ mice, immunostained respectively with β-Gal (green) and IdU (red) antibody, counterstained with DAPI (blue). White arrows indicate the Prx1-CreER-βGal+/IdU+ cells. Mus=muscle; CB= cortical bone; BM= bone marrow; PO= periosteum. Scale bars = 100μm.