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Accumulation of specific extracellular matrix components within chondrospheres (CS) during maturation in vitro: type I collagen (A), type II collagen (B), and aggrecan (C) were dyed brown in 1-day-old, 7-day-old, 14-day-old, and 21-day-old CS sections via immunohistochemical staining. The immunohistochemistry analysis confirmed the absence of type I collagen and gradual increase in levels of hyaline cartilage-specific type II collagen and aggrecan during long-term in vitro incubation.
