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The phosphorylation status of S6 is altered in the cells lacking Bud27. Western blot analysis of the phosphorylated (Rps6-P) and nonphosphorylated (Rps6) S6 RP in both the wild-type and bud27Δ mutant cells. Cells were grown in YPD medium at 30°C (time 0) or in YPD containing 400 ng/mL rapamycin for the indicated times. Anti-phospho (pSer235/236)-S6 RP and anti-S6 RP antibodies were used. Pgk1 was taken as the internal control.
