Fig. 1.

In vitro migration potential, characterization and function of murine MSC and human NSC subpopulations

(a) Migration capacity of oMSC and sMSC over 3 h at two passages (P49 and P60), n=3-6, each data point represents one biological replicate per group; exemplary experiment of 8 independent experiments; cell numbers of migrated oMSC vs. sMSC in two different passages were compared with ANOVA and Bonferroni's multiple comparisons test (***p <0.001). Error bars: SEM.

(b) Migration of oMSC and sMSC over 3 h towards murine cortical (cortex) and hippocampal (HC) primary cultures seeded on the bottom of transwell chambers, n=5, each data point represents one biological replicate per group; cell numbers of oMSC vs. sMSC migrated towards cortical and hippocampal cultures were compared with ANOVA and Bonferroni's multiple comparisons test (***p <0.001). Error bars: SEM.

(c) Migration over 4.5 h of human o/sHB1.F3 Cherry NSC in passage 3 and 4. The number of floating cells in the lower compartment of the migration chamber was counted after crystal violet staining. Cumulative cell number calculated from 79 optical fields within a 24-well plate, n=3 per passage, each data point represents one biological replicate per group out of a single experiment. Cell numbers of migrated oNSC vs. sNSC were compared with two-tailed t-test (*p<0.05); Error bars: SEM.

(d) Two hours after seeding (100,000 cells/19.6 cm²), oMSC and sMSC were tracked every 30 min during 20 h. Velocity, accumulated and Euclidian distance for representative n=9 cells were determined by manual tracking. Each data point represents a single cell analysed per group. Two-tailed t-test was used to compare the velocity, accumulated and Euclidian distance of oMSC vs. sMSC (*p<0.05; **p<0.01); Error bars: SEM.