Fig. 5.

Localisation and quantification of o/sMSC in the brain of AD mouse models

(a) Appearance of eGFP+ oMSC and sMSC (green) in the dentate gyri of 13-month-old 3xTg-AD mice (two upper panels) and 7-month-old APP23xPS45 mice (lower panel). Both animal models show strong expression of APP/Amyloid beta (red). eGFP+ oMSC and sMSC detected in vicinity to DCX positive neurons (red) and circumvent (arrowheads) Aβ plaques (arrows). Scale bars: 100 µm, except for the two microphotographs on the top (50 µm). INA, intranasal application; DCX, doublecortin.

(b) Quantification of eGFP+ oMSC and sMSC in different brain regions of 7-month-old 3xTg-AD mice (n=6 per group) after intranasal application (INA) of eGFP+ o/sMSC (2 x application of 1 × 10⁶ cells). 20 sections from each mouse brain were analysed. oMSC and sMSC cell counts normalized to mm² were compared in each brain region by two-tailed t-test (*p <0.05; **p <0.01; ***p<0.001). Each column represents ≥60 data points obtained from 20 sections (3-10 micrographs per section); Error bars: SEM. HC, hippocampus; OB, olfactory bulb.

(c) Quantification of total eGFP+ o/sMSC and proliferating eGFP/BrdU+ o/sMSC in the HC and CC of 13-month-old 3xTg-AD mice (n=4 per group) after INA. Total o/sMSC were quantified (total oMSC vs. total sMSC) and also compared for their positivity for BrdU (eGFP/BrdU+ oMSC vs. sMSC) from 25 brain sections from each animal by ANOVA and Bonferroni's multiple comparisons test (**p <0.01; ***p <0.001). Each column represents ≥25 data points obtained from 25 sections (1-2 micrographs per section). Error bars: SEM. CC, corpus callosum; HC, hippocampus. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)