Amplification and clinicopathological significance of HER-2 in Kazakh esophageal squamous cell carcinoma

Abstract

Amplification and overexpression of the human epidermal growth factor receptor-2 (HER-2) gene accelerates cell division and proliferation, and promotes tumor growth and metastasis in various malignant tumors. However, there are few reports on its influence and mechanism in esophageal cancer. The aim of the present study was to investigate the gene amplification and clinicopathological significance of HER-2 in Kazakh esophageal squamous cell carcinoma (ESCC). HER-2 gene amplification was detected in 70 esophageal cancer tissues using fluorescence in situ hybridization. The association between the HER-2 gene amplification and the clinicopathological characteristics of patients with esophageal cancer was also analyzed. The amplification rate of the HER-2 gene in patients with esophageal cancer was 54.2% (38/70). The results also revealed a positive association between the amplification rate of the HER-2 gene in esophageal squamous cell carcinomas and the level of tissue differentiation, increasing gradually and significantly among the highly, moderately and poorly differentiated tissues (P<0.05). The amplification rate of the HER-2 gene in patients with lymph node metastasis was higher than those without (P<0.05). There was no significant association between the amplification rate of the HER-2 gene and any of the clinic pathological parameters, such as sex, age, depth of invasion and 3-year survival, among patients (P>0.05). In conclusion, the amplification rate of the HER-2 gene in patients with Kazakh ESCC was high. There was an association with various prognostic factors, including cancer differentiation and lymph node metastasis. HER-2 gene expression levels may be considered as an indicator of poor prognosis in patients with ESCC in the clinical setting, and this may provide a basis of treatment for individualized targeted therapies.

Introduction

Esophageal cancer is one of the highly malignant tumors worldwide. The incidence rate and the mortality rate rank 6th and 4th among all cancers in China, respectively (1), 95% of all esophageal cancer cases are cases of squamous cell carcinoma (2). There are regional and ethnic variations in the incidence rate and etiological factors behind esophageal cancer. The variation between different nationalities and geographical regions are likely to be related to genetic susceptibility (3). Xinjiang is a multi-ethnic residential area and is an area with high incidence for esophageal cancer, and Kazakh populations present the highest risk of esophageal cancer in the area. The prognosis of esophageal cancer types has been improved by surgery, chemotherapy, radiotherapy, targeted therapy and immunotherapy; however, the prognosis is still unsatisfactory. The overall 5-year survival rate is less than 20% (4). Therefore, early diagnosis, early treatment and new treatment methods are needed to improve the recovery rate and the 5-year survival rate. Targeted therapy is an important step in the development of individualized treatment in for patients with esophageal cancer.

Human epidermal growth factor receptor-2 (HER-2) is a member of the epidermal growth factor receptor (EGFR) family, and it is a transmembrane tyrosine kinase receptor with a molecular weight of 185 kDa. Overexpression of the HER-2 protein in malignant tumor cells accelerates cell division and proliferation, and ultimately promotes tumor growth and metastasis (5). In recent years, HER-2 receptors and the whole HER-2-mediated signaling pathway have been identified as targets in the treatment of various malignant tumors (6).

Fluorescence in situ hybridization (FISH) is a molecular pathological technique used to detect chromosome aberration, gene deletion and amplification by using special fluorescent labeled DNA single-stranded-nucleates as probes. It is widely used in the detection of various cancers such as leukemia, lung cancer, breast cancer and renal cancer. Due to its high sensitivity and specificity, it can qualitatively detect malignant cells (7,8). FISH is recognized as the gold standard for detecting the HER-2 gene (9). There are significant regional and ethnic variations in esophageal cancer rates worldwide; however, there is currently no reports on the HER-2 gene of Kazakh patients with esophageal cancer. Thus, the current study detected HER-2 gene amplification in esophageal squamous cell carcinoma (ESCC) tissues, and analyzed the association between HER-2 gene amplification and the clinicopathological characteristics of ESCC patients. This is to provide a theoretical basis for the research, diagnosis and treatment of ESCC patients in Kazakh populations, to explore the association between HER-2 gene amplification and the survival period of patients with esophageal cancer after radical operation, and to evaluate the association between HER-2 gene expression levels and the prognosis of patients with ESCC.

Patients and methods

Patients

A total of 70 specimens were obtained from Kazakh patients who had not received chemotherapy or radiotherapy prior to surgery from the Department of Thoracic Surgery of the First Affiliated Hospital of Xinjiang Medical University, China, from January 2014 to January 2016. There were 54 males and 16 females, with a median age of 55.5 years (range of 32-79 years). Postoperative pathological diagnosis was confirmed as ESCC, and according to the 7th edition of AJCC Cancer Staging Manual (10), 17 cases were poorly differentiated, 39 cases were moderately differentiated and 14 cases were highly differentiated. There were 41 cases of positive lymph node metastasis vs. 29 cases of negative lymph node metastasis; 7 cases had IA-IB stage, 39 had IIA-IIB stage and 24 had IIIA-IIIB stage disease. All patients undergoing surgery had written consent to use their tissues for this study. The collection of samples conformed to the ethical requirements and this study was approved by the Ethics Committee of the First Affiliated Hospital of Xinjiang Medical University.

FISH and score for HER-2/neu

FISH analysis was performed in the Department of Pathology of the First Affiliated Hospital of Xinjiang Medical University, using the PathVysion HER-2/neu (Vysis) kit composed of 2 hybridization probes: A DNA probe CEP17 of 5.4 kb for the centromeric region of chromosome 17, marked with Spectrum Green and a DNA locus-specific probe LSI HER2/neu of 190 Kb, marked with Spectrum Orange for HER2/neu.

Fresh cancer tissue was applied to the slide and was dried at room temperature (25˚C) for 24 h, then was preserved at -80˚C. After decomposition and denaturation with 70% formamide solution, specimens were dehydrated in 70, 85 and 100% ethanol series. The ThermoBrite automatic in situ hybridization system was used, the hybridization conditions were as follows: Denaturation at 75˚C for 5 min, hybridization at 37˚C for 16 h. The next day, slides were washed with 2xSSC solution for 2 min at 72˚C and then at room temperature for 1 min. Slides were allowed to dry in the dark. After the slides were dried, 10 µl DAPI II was added and the glass was covered. Ultimately this was observed using fluorescence microscopy.

A special image acquisition and analysis system (Leica Microsystems, Ltd.) was used to count the number of signals under a fluorescence microscope. The counting results were independently completed by two participants, and the results were confirmed only when the counts were consistent.

Results criteria: At least 100 nuclei were counted. When the ratio of red signal (HER2-neu) to green signal (17 chromosome centromere) was ≥2.0, or the number of nuclei of cells with >15 red signals for >10% of the total number of cells, HER2-neu was considered to be amplified. When the ratio of red signal to green signal was less than 1.8, HER2-neu was considered to not be amplified; if the ratio of red signal to green signal was between 1.8 and 2.0, a total of 100 extra cells were counted (11).

Statistical analysis

For statistical analysis a χ² test was used to evaluate the differences between the two groups, the survival analysis was performed by the Kaplan-Meier with log-rank test, and in all tests P<0.05 was considered to indicate a statistically significant difference. Statistical calculations were performed using SPSS 20.0 (IBM Corp.).

Results

HER2 amplification pattern

The FISH technique was used to detect HER-2 gene expression levels in 87 fresh specimens of ESCC. Among them, results from 70 cases were recorded, with the remaining 17 being excluded due to failure of the experiment, nuclear rupture and unclear nuclear staining. In the current study, the positive amplification rate of HER-2 was 54.2% (38/70). Normal epithelial cells and non-neoplastic stroma or inflammatory cells generally presented two HER-2 signals. There were two patterns observed in the HER-2 gene amplification in esophageal cancer samples of Kazakh populations: >15 red signals (HER-2) and multiple green signals (CEP17) in >10% of tumor cell nucleus were amplified, and ≥4 red signals (HER-2) and green signals (CEP17) in >40% of the tumor cells that showed as polysomy, were amplified (HER2/CEP17 ratio ≥2.0, Table I and Fig. 1).

Table I.

Results of HER-2 and CEP17 by FISH.

			Ploidy No.
Case	Age	Sex	2 HER-2/CEP17	3 HER-2/CEP17	4 HER-2/CEP17	>15 HER-2/CEP17	FISH
1	51	M	62/54	38/46	0/0	0/0	N
2	65	M	48/42	32/39	20/19	0/0	N
3	45	M	44/44	46/34	10/22	0/0	N
4	54	F	54/38	30/32	16/30	0/0	N
5	50	M	38/22	58/38	4/40	0/0	N
6	51	F	10/48	14/28	66/24	10/0	P
7	41	M	62/48	38/28	0/24	0/0	N
8	63	M	4/34	36/15	60/25	0/26	P
9	57	M	8/60	36/14	56/20	0/0	P
10	46	M	34/22	12/60	54/18	0/0	P
11	56	M	24/46	56/20	8/32	12/2	P
12	68	M	28/38	40/26	42/36	0/0	N
13	63	M	12/44	18/28	70/28	0/0	P
14	66	M	14/42	24/38	52/20	10/0	P
15	71	M	54/40	24/12	22/48	0/0	N
16	44	M	28/46	34/30	38/24	0/0	N
17	69	M	56/22	22/18	8/56	14/4	P
18	63	M	48/34	14/46	38/20	0/0	N
19	68	M	36/23	53/46	2/31	0/0	N
20	54	M	5/34	35/40	60/26	0/0	P
21	59	M	54/62	23/0	23/38	0/0	N
22	66	M	7/55	50/35	33/10	10/0	P
23	64	F	20/10	57/50	20/40	0/0	N
24	50	M	64/60	21/14	15/26	0/0	N
25	38	F	6/36	40/46	54/18	0/0	P
26	62	M	70/70	21/0	9/22	0/8	N
27	57	M	5/56	55/22	40/12	0/10	P
28	67	F	12/41	40/45	34/14	14/0	P
29	52	M	64/49	24/19	12/32	0/0	N
30	47	M	10/23	49/67	38/10	0/0	P
31	50	M	70/60	24/16	6/24	0/0	N
32	52	M	15/34	31/40	44/10	0/0	P
33	49	M	10/30	43/56	35/8	12/2	P
34	63	M	63/49	24/21	13/30	0/0	N
35	57	M	5/30	39/61	45/9	11/0	P
36	62	M	58/66	19/3	25/31	0/0	N
37	57	M	41/50	36/16	23/34	0/0	N
38	57	F	45/59	25/13	20/28	0/0	N
39	62	F	58/55	14/21	28/24	0/0	N
40	69	F	4/19	56/71	40/10	10/0	P
41	45	M	6/47	36/28	58/20	0/5	P
42	43	M	67/48	10/0	16/21	7/8	N
43	58	F	4/20	30/54	54/14	12/0	P
44	66	M	69/50	24/10	6/24	1/16	N
45	57	M	64/49	20/19	16/32	0/0	N
46	79	M	12/54	30/18	48/18	10/0	P
47	57	M	12/40	22/38	54/20	12/2	P
48	54	F	44/38	34/16	22/46	0/0	N
49	61	F	58/44	18/32	24/24	0/0	N
50	51	M	12/28	16/44	68/28	6/0	P
51	63	M	55/48	22/38	23/14	0/0	N
52	69	M	18/40	10/32	72/28	0/0	P
53	73	M	5/14	8/24	76/22	11/1	P
54	51	M	12/60	34/20	54/20	0/0	P
55	68	M	16/21	24/58	48/18	12/3	P
56	66	M	16/50	18/26	66/24	0/0	P
57	63	M	21/30	22/58	46/12	11/0	P
58	57	F	8/36	20/41	58/20	14/3	P
59	32	M	18/28	12/48	60/24	10/0	P
60	56	M	22/44	24/40	54/16	0/0	P
61	66	F	16/30	22/46	62/24	0/0	P
62	61	F	16/34	20/44	52/22	12/0	P
63	64	M	40/32	36/48	24/20	0/0	N
64	77	F	56/60	24/30	20/10	0/0	N
65	53	M	62/58	38/42	0/0	0/0	N
66	49	F	21/32	16/48	52/20	10/0	P
67	63	M	33/40	6/38	61/22	0	P
68	52	M	56/48	34/44	10/8	0/0	N
69	75	M	12/26	16/29	72/30	0/5	P
70	70	M	58/42	36/40	6/18	0/0	N

FISH, fluorescence in situ hybridization; P, Positive amplification; N, No amplification.

Figure 1.

Fluorescent in-situ hybridization detection of HER-2 gene amplification in esophageal cancer tissues. (A) Tissue exhibiting a normal pattern of HER-2/neu gene (2 red spots) and chromosome 17 probe (2 green spots). (B) Tissue demonstrating amplified HER-2/neu gene (amplification of the ratio between HER-2/neu signals and CEP17 signals, >2.2). (C) Tissue demonstrating HER-2/neu gene amplification (>15 HER-2/neu signals and multiple CEP17 signals in >10% of the tumor cell nucleus).

Association between HER2 gene amplification and clinicopathological characteristics

The amplification rates of the HER-2 gene were 14% (2/14), 54% (21/39) and 88% (15/17) among the highly differentiated, moderately differentiated and in poorly differentiated groups, respectively. Statistical differences were observed in different degrees of differentiations (P<0.001). The amplification rate of the HER-2 gene in the lymph node metastasis group was higher than that of the non-lymph node metastasis group (P<0.010); However, there were no significant differences in the HER-2 gene positive amplification among age, sex, depth of invasion, clinical stage and vascular infiltration status (P>0.05; Table II).

Table II.

Relationship between HER-2 gene amplification and clinicopathological characteristics of patients with esophageal squamous cell carcinoma.

		HER-2 amplification, n (%)
Variable	Case	Positive	Negative	χ2	P-value
Sex				0.032	0.544
Male	54	29(54)	25(46)
Female	16	9(56)	7(44)
Age (years)				0.048	0.508
<60	36	20(56)	16(44)
≥60	34	18(53)	16(47)
Differentiation				16.925	<0.001
Well	14	2(14)	12(86)
Moderate	39	21(54)	18(46)
Poor	17	15(88)	2(12)
Depth of invasion				1.228	0.193
T1-2	30	14(47)	16(53)
T3-4	40	24(60)	16(40)
Lymph node metastasis				6.557	0.010
Yes	41	17(41)	24(59)
No	29	21(72)	8(28)
pTNM				2.256	0.105
I, II	46	22(48)	24(52)
III, IV	24	16(67)	8(33)

pTNM, pathological stage.

Association between HER2 gene amplification and survival

Only 42 patients had complete follow-up records out of the total of 70 patients. The follow-up period was 36 months. At the time of the statistics, 22 patients had succumbed to the disease and 20 patients remained alive. Kaplan-Meier univariate survival analysis demonstrated that there was no significant association between HER-2 gene amplification and the survival of ESCC patients (P>0.05; Fig. 2).

Figure 2.

Relationship between HER-2 gene amplification and survival.

Discussion

With the development of cancer molecular biology, targeted therapy has entered the clinical treatment stage for various cancer types, to provide an effective treatment for patients with malignant tumors, including esophageal cancer (12-14). A large number of clinical studies have demonstrated that HER-2 is a therapeutic target for breast cancer and the efficacy of herceptin for treating patients with HER-2-positive breast cancer is significantly better than conventional chemotherapy, and this consensus has been reached in the treatment of breast cancer (15).

Studies have shown that the HER-2 gene is associated with the histological types, lymph node metastasis and prognosis of various malignant tumors (16). In the past few years, a large number of studies on HER-2 gene amplification or protein overexpression in epithelial malignant tumors such as gastric cancer, lung cancer and breast cancer have been published (17-20). However, there are few reported studies examining HER-2 gene amplification in esophageal cancer. In the present study, HER-2 amplification was examined in ESCC specimens of Kazakh populations, then the association between HER-2 amplification and clinicopathological characteristics of ESCC was analyzed. The aim of the study was to provide theoretical evidence for the targeted therapy of esophageal cancer.

Among the studies of esophageal cancer in different regions and ethnicities, the HER2 gene amplification rates have been recorded as 6.5-30% (21-22). In the present study, the HER-2 gene amplification rate was 54.2% in Kazakh patients, which was significantly higher than that reported in other nationalities. Whether this is related to the life habits of Kazakhs, for example heavy drinking, smoking, eating large quantities of smoked horse meat, high-salt diet, low vitamin consumption or other esophageal cancer-inducing factors or ethnicity is unclear, and this needs further study for verification.

There are also different conclusions about the association between HER-2 gene amplification and clinicopathological characteristics of ESCC. By using FISH, Reichelt et al (23) detected that the HER-2 gene amplification levels in ESCC had no association with clinicopathological features. Zhan et al (24) reported that the HER-2 amplification was related to the differentiation and staging of cancer tissues. The results of the present study showed that there were significant differences in the degree of differentiations and lymph node metastasis in ESCC. Niemiec et al found that HER-2 promotes the infiltration and/or metastasis of tumor cells by increasing the secretion of matrix metalloproteinase and changing the tissue structure (25). The current study suggests that the HER-2 gene was closely associated with the occurrence and development of esophageal cancer and that HER-2 gene expression indicated poor prognosis of esophageal cancer to some extent. However, the Kazakh people are a nomadic people, most of them living in mountain areas and without a stable phone number, so the proportion of lost follow-up is a little bit high, even though the follow-up rate was 60%, so these conclusions need to be further confirmed by larger samples study. Besides, there was no significant association between HER-2 gene amplification and clinicopathological parameters such as sex, age, depth of invasion and clinical stage. In addition, the amplification of the HER-2 gene was not related to the survival period of patients, and this result may be due to such factors including the limited numbers of samples, different geographical regions and targeted population. The amplification of the HER-2 gene may be associated with the following factors: i) The number of follow-up patients is small; ii) The target population of the living area and the ethnicity varied; iii) The regional economic development level, medical conditions, diagnosis, treatment, patients' education level and positive treatment views.

In conclusion, the amplification rate of HER-2 in patients of Kazakh nationality with ESCC is significantly higher than that of other nationalities, and it is related to the degree of cancer differentiation and lymph node metastasis. The HER-2 amplification rate may have potential clinical significance in the prognostic evaluation of esophageal cancer, and it can also be used as a potential target therapy. But there are still some limitations in our study, because the manuscript was focus at the association between HER2 amplification and clinicopathological parameters and prognosis of esophageal cancer, and the association between HER2 expression and esophageal cancer pathological parameters has been reported in many literatures, so we only analyze the amplification level under FISH detection. However, the evidence behind HER-2 amplification as an independent prognostic factor for patients with esophageal cancer is still insufficient, this needs to be further validated by large-sample, multi-center and long-term follow-up studies.

Funding

The present study was funded by the Natural Science Foundation of Xinjiang Uygur Autonomous Region (grant no. 2016D01C264).

Availability of data and materials

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Authors' contributions

AM and EA was designed the current study, performed the experiments, drafted the manuscript and collected various clinical data, including clinicopathological and patient follow-up data. JA was analyzed and interpreted the data, performed statistical analysis and assisted with the experiments. MN and ZL provided study materials, revised the manuscript critically and designed the experiments of the current study. All authors read and approved the final manuscript.

Ethics approval and consent to participate

All experiments were approved by The Ethics Committee of The First Affiliated Hospital of Xinjiang Medical University, and written informed consent was obtained from all participants.

Patient consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.