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. Author manuscript; available in PMC: 2020 Sep 23.
Published in final edited form as: Biochemistry. 2020 Aug 30;59(36):3300–3315. doi: 10.1021/acs.biochem.0c00608

Figure 3: Mass spectra of the C-terminus redox center peptide for the WT and (αMe)Sec-containing mutant.

Figure 3:

(A) Mass spectrum of peptide SGLEPTVTGCUG that results from a trypsin digest. (B) Mass spectrum of peptide SGLEPTVTGC(αMe)UG that results from a trypsin digest. The mass peaks differ by 14 daltons due to the presence of the αMe group. The sample were not reduced and alkylated prior to MS analysis, so Cys/Sec residues exist in the oxidized form as a selenosulfide bridge.