Skip to main content
PMC home page
Cell Death & Disease logoLink to Cell Death & Disease
. 2020 Sep 24;11(9):797. doi: 10.1038/s41419-020-02998-6

PI3K/AKT pathway as a key link modulates the multidrug resistance of cancers

Rui Liu 1, Youwen Chen 1, Guangzhi Liu 1, Chenxi Li 1, Yurong Song 1, Zhiwen Cao 1, Wen Li 1, Jinghong Hu 1,✉, Cheng Lu 2,✉, Yuanyan Liu 1,✉
PMCID: PMC7515865  PMID: 32973135

Abstract

Multidrug resistance (MDR) is the dominant challenge in the failure of chemotherapy in cancers. Phosphatidylinositol 3-kinase (PI3K) is a lipid kinase that spreads intracellular signal cascades and regulates a variety of cellular processes. PI3Ks are considered significant causes of chemoresistance in cancer therapy. Protein kinase B (AKT) is also a significant downstream effecter of PI3K signaling, and it modulates several pathways, including inhibition of apoptosis, stimulation of cell growth, and modulation of cellular metabolism. This review highlights the aberrant activation of PI3K/AKT as a key link that modulates MDR. We summarize the regulation of numerous major targets correlated with the PI3K/AKT pathway, which is further related to MDR, including the expression of apoptosis-related protein, ABC transport and glycogen synthase kinase-3 beta (GSK-3β), synergism with nuclear factor kappa beta (NF-κB) and mammalian target of rapamycin (mTOR), and the regulation of glycolysis.

Subject terms: Cancer metabolism, Targeted therapies

Facts

  • Several possible mechanisms are related to the occurrence and progression of MDR in cancer, including the overexpression of efflux pumps, abnormal tumor microenvironment and other cellular/physiological pathways.

  • Activated PI3K/AKT catalyzes the phosphorylation of a series of proteins, promotes tumor cell growth and proliferation, inhibits apoptosis, promotes invasion and metastasis, regulates endothelial cell growth and angiogenesis, and increases the sensitivity to radiation.

  • Many types of tumors often carry at least 1 change in PI3K.

Open questions

  • What is the role of the PI3K/AKT pathway in MDR?

  • The linkage between PI3KAKT pathway and ABC transporter as well as their synergistic mechanisms need to be studied in-depth to reveal the mechanism of MDR.

  • The exact modulation mechanisms of the PI3K/AKT pathway in reversing MDR in a specific manner in tumor cells to lock their growth and metastasis, and induce apoptosis must be deeply investigated in the future.

Introduction

Although important advances in the field of chemotherapy have lowered the mortality rate for cancer patients, the 5-year survival rate remains gloomy, largely due to intrinsic or acquired mechanisms of resistance to antineoplastic drugs1. Multidrug resistance (MDR) describes a phenomenon that leads to resistance to the administered drugs and other drugs with completely different structure and mechanism of actions2. There are many mechanisms involved in the generation of MDR. One mechanism is the destruction of apoptosis via the abnormal expression of apoptosis-related factors, which makes cells resistant to drug-induced cell death. A change in the cell cycle process may cause the proliferation of cancer cells and promote resistance3. The mechanism classical is to target anticancer drug transport across the cell membrane by increasing the activity of efflux pumps, such as adenosine triphosphate (ATP)-binding cassettes (ABC) transporters4. Cancer cells exhibit a special metabolic phenotype—aerobic glycolysis that rapidly transports and consumes glucose to produce ATP and promote drug efflux5,6.

The PI3K/AKT pathway is activated by the production of 3’-phosphorylated phosphoinositides (Fig. 1), and it is an important signaling pathway for MDR in a variety of cancers, such as breast cancer, leukemia, lung cancer, ovarian cancer, hepatocellular carcinoma, and melanoma7–12. Many types of tumors often carry at least one change in PI3K13–17 (Table 1). The MDR phenotype often accompanies activation of the PI3K/AKT pathway, which renders a survival signal to withstand cytotoxic anticancer drugs and enhances cancer stem cell (CSC) characteristics. However, activation of the PI3K/AKT pathway alone is not responsible for MDR in many cases, and synergistic transduction with up/downstream targets is required. Notably, the PI3K/AKT pathway is a key link that synergizes many of the targets involved in incorporating the modulation of apoptosis, cell growth, and cellular metabolism that are associated with the mechanism of MDR.

Fig. 1. As a major downstream effector of receptor tyrosine kinase (RTK) and G protein-coupled receptors, PI3K activates various downstream effectors by generating phospholipids, transducing signals of various growth factors and cytokines into intracellular information.

Fig. 1

Open in a new tab

The main lipid substrate of PTEN is PIP 3 and indeed PTEN acts as a negative regulator of PI3K/AKT signaling. Among the upstream signaling networks, Akt inactivate TSC1/2 and activate mTORC1. mTORC2 directly phosphorylates Akt at S473 residue leading to its complete activation. This activation of the PI3K/Akt pathway is opposed by PTEN.

Table 1.

Frequency of PI3K pathway alterations in cancer.

Cancer type Altered genes
PIK3CA (%) PTEN (%) AKT1 (%)
Breast cancer 40 2–4 2–3
Endometrial cancer 68 43 3
Lung cancer 30 4 4
Squamous cell carcinoma 42.70 3 2
Ovarian cancer 68 19 27
Open in a new tab

Survival signals are different from apoptosis resistance and protect cancer cells from death. Therefore, the signaling involved in apoptosis resistance and increased survival are the main regulators of MDR in chemotherapy18. For example, many cell life and death signals are regulated by Bcl-2 family proteins and X-linked apoptosis proteins (XIAP), which converge in the mitochondria, where the fate of the cell is ultimately decided. Resistant cells express higher levels of Bcl-2 and/or XIAP19. Abnormal activation of the PI3K/AKT pathway counteracts the chemotherapeutic-induced apoptosis via the enhancing of anti-apoptotic genes, such as Bcl-2 and XIAP, and reducing of pro-apoptotic genes, such as Bax, to modulate MDR20.

PI3K binds to the PH domain of AKT and induces conformational changes and the subsequent phosphorylation of AKT, and activated AKT moves from the cytoplasm to the cell membrane. This cascade directly or indirectly activates its downstream molecular proteins, such as NF-κB and mTOR21. NF-κB is the main regulator of gene transcription and its upregulation in chemotherapy-resistant cell lines is related to treatment failure22. The development of MDR is related to the PI3K/AKT pathway partially due to the activation of NF-κB23. MDR may be due to activation of PI3K/AKT/NF-κB, which develops into the transformation of cyclin D1 and the expression of G1/S, and an acceleration of the cell cycle process24. The abnormal activation of PI3K/AKT/NF-κB also regulates the expression of P-gp to achieve MDR. As mentioned earlier, P13K/AKT/mTOR affected dysregulation of micro-RNA (miRNA) may also underlie MDR in tumor cells. MDR regulation of miRNA-mediated malignant tumorigenesis is an important link25,26. GSK-3β is a kinase that regulates proliferation in response to various stimuli. It is present in a variety of cancer types, and it involves multiple molecular pathways that evade chemotherapy, radiation therapy, and targeted therapy27. PI3K/AKT phosphorylates GSK-3β into an inactive form, which may cause an increase in β-catenin that translocates to the nucleus where it acts as a transcription factor to increase the expression of target genes involved in MDR28.

Previous studies showed that the PI3K/AKT pathway enhanced the biological basis of cancer by effectively expressing ABC transporters containing p-glycoprotein (P-gp, ABCB1), multidrug resistance-associated protein 1 (MRP1, ABCC1), and breast cancer resistance protein (BCRP, ABCG2), whose activation may reduce the response to chemotherapy drugs and enhance the drug efflux29,30. Due to the acidified micro-environment in tumor cells, aerobic glycolysis may confer MDR to cancer cells, which may reduce drug absorption and efficiency, and/or increase the content of intermediate metabolites31,32. Reducing the cellular energy of glycolytic metabolism may reduce drug resistance by reducing the outflow of drugs from cells. Notably, the PI3K/AKT pathway is involved in the regulation of aerobic glycolysis to increase energy supply, which enhances the ability of ABC transporters to excrete drugs33. The MDR phenotype is generally accompanied by an abnormal activation of the PI3K/AKT signaling pathway. In brief, the PI3K/AKT pathway is a key link in MDR via cooperation with upstream and downstream targets, which provides a survival signal for anti-cancer drugs.

The PI3K/AKT pathway is very complex and may have many variations and be affected by a large number of diverse inputs, which influence the MDR processes. A better understanding of PI3K/AKT synergism with other targets is important to further strengthen the rationale for cotargeted treatment strategies and combination therapy to improve the efficacy of PI3K/AKT inhibitors in the clinic.

PI3K/AKT modulates the expression patterns of the apoptosis-related MDR process

The PI3K/AKT pathway is important for MDR in certain types of cancer, and it is a hub that influences MDR via the inhibition of apoptosis34. The PI3K/AKT pathway participates in the apoptotic process, which is inseparable from apoptosis-related factors, such as the Bcl-2 family and XIAP. The present study discussed how the PI3K/AKT pathway affects the MDR process in regulating apoptosis-related factors in detail.

Bcl-2 family

Numerous studies suggested that abnormal activation of the PI3K/AKT signaling pathway contributed to the upregulation of Bcl-2 expression, which leads to apoptosis-mediated MDR in many cancer therapies35. The Bcl-2 protein family is a key factor in apoptosis, and it is an ancient cell suicide program that is important for cancer development and drug response36. Bcl-2 proteins are located on the mitochondrial membrane, including Bad, Bax, Bcl-2, Bid, and Bcl-xL, and may alter mitochondrial membrane permeability and trigger the release of cytochrome c or caspase leading to apoptotic cell death via activation of postmitochondrial caspase cascades. Anti-apoptotic proteins, such as Bcl-xL and Bcl-2, stimulate cell survival by preventing the release of cytochrome c from mitochondria, and pro-apoptotic proteins, such as Bax and Bad, induce intracellular mitochondria to release cytochrome c37. The balance between proteins of the Bcl-2 family plays a vital role in the apoptotic pathways induced by several foreign stimuli of PI3K/AKT38. The balance between these opposing apoptosis proteins, such as a simple “varistor”, regulates the sensitivity of cells to apoptotic stress39. The apoptotic regulator Bcl-2 is a key component of the anti-apoptotic mechanism. Bcl-2 is an oncogene that blocks mitochondrial outer membrane permeabilization and inhibits apoptosis, and it is always overexpressed in MDR of cancers. Overexpression of Bcl-2 typically results in cancer cell resistance to cancer factors, which is associated with abnormal changes in PI3K/AKT pathways40,41.

Activated AKT directly phosphorylates Ser136 of Bad (the target of AKT)42. AKT activates PAK1, which in turn phosphorylates Bad at Ser-112 and causes its release from the Bcl-xL complex to inhibit apoptosis. Activated AKT promotes cell survival via activation of Bcl-2 and inhibition of Bax43. The mitochondrial translocation of Bad and the physiological interaction between Bad and Bcl-xL also induce mitochondrial dysfunction, and the PI3K/AKT-mediated interaction between Bad and Bcl-xL maintains mitochondrial integrity and prevents cytochrome c efflux44. Therefore, PI3K activity is important for the retention of Bax in the cytoplasm, and AKT inhibits Bax translocation to the mitochondria, which is related to apoptosis-mediated MDR45. In summary, activation of the PI3K/AKT pathway promotes MDR via the regulation of a variety of cellular apoptotic processes (Bax/Bcl-2).

XIAP

XIAP is an intracellular anti-apoptotic protein that plays a vital role in cell survival. It is overexpressed in many cancers, and it is involved in tumor drug resistance46. Notably, the PI3K/AKT pathway triggers XIAP-induced apoptosis, which may be involved in tumor cell proliferation and metastasis-mediated MDR47.

XIAP is one of the main inhibitors of apoptosis, and it blocks the intrinsic and extrinsic apoptosis pathways. High expression of XIAP was noted in ovarian cancer cells, and it is involved in chemoresistance48. Abnormal activation of the PI3K/AKT signaling pathway induces XIAP expression49. Kahana et al. found that downregulation of AKT reduced the expression level of XIAP50, which directly inhibited the activity of caspase and regulated apoptosis via a variety of pathways. XIAP is the only member of IAP famliy that blocks active caspase and inhibits apoptosis via binding to the initiator caspase (caspase-9) and the effector caspase (caspase-3)51. XIAP contains three baculovirus IAP repeat (BIR) domains that interact with caspase. XIAP upstream caspase-9 via its BIR3 domain, and it inhibits downstream caspase-3 and caspase-7 via its BIR2 domain52. XIAP is further inhibited with AKT downregulated, which eventually leads to termination of caspase activation53, and reversed MDR in many type of cancers. The ubiquitin E3 ligase activity of XIAP mediates the proteasome-dependent degradation of caspase, which is required for anti-apoptotic function54.

Previous studies demonstrated that XIAP was downstream of AKT and prevented apoptosis via the upregulation of the PI3K/AKT cell survival signaling pathway. The overexpression of XIAP using adenovirus increased phosphorylated XIAP, and increased the content of phosphorylated AKT (indicating AKT activation), which was associated with decreased MDR of cisplatin-induced apoptosis55. Dan et al. showed that AKT stabilized the phosphorylation of XIAP at Ser87 and protected XIAP from cisplatin-induced degradation56. XIAP also controlled serum starvation-induced autophagy downstream of the PI3K/AKT pathway. XIAP inhibited autophagy via regulating the level of cytosolic p5357. When serum induced autophagy, AKT deactivation led to dephosphorylation of XIAP, which caused XIAP to dissociate from murine double minute2 (MDM2). The kinase enhanced the ubiquitination degradation of p53 by Mdm2 and promoted the occurrence of autophagy. Therefore, the interaction of PI3K/AKT signaling pathway activation and its phosphorylation of downstream target protein of XIAP may be responsible for the mechanism of MDR due to autophagy-induced apoptosis.

Aberrant activation of the PI3K/AKT/NF-κB and PI3K/AKT/mTOR pathways are associated with MDR

Numerous studies suggested that the PI3K/AKT pathway was the network with the highest mutation frequency in human cancers34. PI3K/AKT/NF-κB and PI3K/AKT/mTOR are the two main mutated pathways involved in apoptosis and tumorigenesis, and these pathways are related to MDR. The dysregulation of the major components of these two signaling pathways that leads to the activation of certain targets to promote MDR in many types of cancers is discussed below58.

PI3K/AKT/NF-κB

The constitutive PI3K/AKT/NF-κB pathway is upregulated in chemoresistance-resistant cell lines and leads to treatment failure, which may be related to the inhibition of apoptosis and promotion of tumor growth by NF-κB59. NF-κB is a transcription factor with multiple regulatory functions, and it typically consists of a p50–p65 heterodimer60. In the resting state of most cells, NF-κB binds to the cytoplasmic inhibitor IκB-α. According to the classical pathway, phosphorylated IκBα rapidly degrades and releases NF-κB which is phosphorylated and translocated to the nucleus. This data suggest that the PI3K/AKT signaling pathway is involved in the modulation of IκBα via the induction of IKK phosphorylation. PI3K/AKT triggered the activation of NF-κB via enhancing the transcriptional activity of the p65 subunit61. Activation of PI3K/AKT inhibits apoptosis by stimulating the transactivation potential of the RelA/p65 protein. Studies also showed that the PI3K/AKT/NF-κB pathway led to activation of the AKT-mediated transcription factor c-AMP response element-binding protein (CREB) or IKK, which produced further activation of the NF-κB-exerted anti-apoptotic effects62. Although many studies showed that the PI3K/AKT pathway activated the NF-κB system, the main details of its molecular mechanism must be further studied63.

Evidence showed that the development of MDR was related to the PI3K/AKT pathway, at least in part due to the activation of NF-κB64–66. The MDR phenotype of breast cancer cells was associated with abnormal activation of the PI3K/AKT/NF-κB signaling pathway67. Constitutive and inducible activation of PI3K/AKT/NF-κB in cancer cells (such as pancreatic cancer) are closely related to tumorigenesis and promoted MDR-like cell proliferation and apoptosis68. Ni et al. reported that MDR of nasopharyngeal carcinoma (NPC) was reversed by inhibition of the PI3K/AKT/NF-κB signaling pathway. It primarily promoted the expression of p53 and Bax protein in NPC HK-1 cells via regulation of the PI3K/AKT/NF-κB pathway and inhibited the expression of cyclin D protein69. NF-κB promotes cells to pass the G1/S checkpoint by stimulating transcription of cyclin D1, which accelerates cell progression, promotes tumor growth, and leads to drug resistance24. The PI3K/AKT/NF-κB pathway affected the expression of Bcl-2 and caspase-3, which was cleaved by apoptotic proteins. These alterations regulated NSCLC cell growth and apoptosis to promote MDR70. Abnormal activation of the PI3K/AKT/NF-κB signaling pathway also regulated the expression of P-gp to achieve MDR, which is discussed in the following sections23. In summary, abnormality of the PI3K/AKT/NF-κB signaling pathway is a common occurrence in cancer, and it is involved in the development of MDR. It is also used as a target for reversing resistance.

PI3K/AKT/mTOR

The PI3K/AKT/mTOR signaling pathway plays a vital role in a variety of biological and physiological processes, and survival, growth transcription, and translation, are mostly related to the occurrence of MDR71,72. Abnormal activation of the PI3K/AKT/mTOR pathway helps tumors to produce MDR, such as acute myeloid leukemia (AML) and ovarian cancer73,74.

Activated AKT directly activates mTORC1 via the phosphorylation of mTOR at Ser2448. The phosphorylation of tuberous sclerosis complex 2 (TSC2) by AKT reduces TSC1 and TSC2, which activates mTORC1. When TSC2 was inactivated by AKT, Rheb-GTP stimulated mTORC1 activity and subsequently phosphorylated 70S6K1, S6. and eukaryotic translation initiation factor 4E-binding protein 1 (4EBP1)73,75. Phosphorylation of 4EBP-1 also promotes the translation of mRNA encoding hypoxia-inducible factor 1α, cyclin D1, and c-Myc, which leads to angiogenesis or cell cycle progression. Abnormalities in the PI3K/AKT/mTOR pathway promote MDR via the above-mentioned mechanisms76. However, some studies revealed a PI3K-dependent mechanism for mTORC2 activation, which allowed mTORC2 to activate AKT in a manner that was regulated temporally and spatially by PtdIns(3,4,5) P3. AKT activates mTORC2 via the phosphorylation of mSin1 at T86, in turn the activated mTORC2 stimulates Akt through phosphorylation of Akt at S473, which forms a positive feedback regulatory loop77,78. Activation of mTORC2 controls cell growth via the modulation of various physiological processes, such as fat production, apoptosis, and glucose metabolism75. The targeting of mTORC1 is a good method to reverse MDR. However, the inhibition of mTORC1 and mTORC2 must be targeted to prevent mTORC2 from compensatory activation of AKT79.

The P13K/AKT/mTOR pathway is affected by miRNAs that act as negative transcriptional regulators of target genes with different potential roles in tumorigenesis and behavior25,26. These generally cause mutations in PIK3CA, PTEN (phosphatase and tensin homolog deleted on chromosome 10), and AKT, which are important components of the P13K/AKT/mTOR signaling pathway, and eventually lead to MDR80. Yue et al. found that overexpression of miR-182 reduced the resistance of trastuzumab in trastuzumab-resistant cells, partially due to inactivation of the PI3K/AKT/mTOR signaling pathway81. PI3K/AKT/mTOR is an essential path to MDR via the modulation of miRNAs-mediation of the tumorigenesis process of malignant cells. Similarly, long non-coding RNAs also play an important role in drug resistance. The development of MDR is promoted by high expression of XLOC_006753, and its development is activated by the PI3K/AKT/mTOR signaling pathway in GC cells82. LncRNA-HOTAIR silencing significantly reduced the phosphorylation of PI3K, which suggests that knocking down lncRNA-HOTAIR effectively reduced the resistance of breast cancer cells to DOX via inhibition of the PI3K/AKT/mTOR signaling pathway83. The PI3K/AKT/mTOR pathway is complicated. Although, it can change a lot, and it can be affected by a large number of different factors that promote MDR.

PI3K/AKT phosphorylates GSK-3β to promote tumor proliferation related to MDR

GSK-3β is a kinase that modulates proliferation in response to various stimuli, and it is one of the subtypes of GSK-3 (a serine/threonine kinase)84. Dysfunction of GSK-3β is an active element in cell proliferation that is associated with cancer, and it is overexpressed in certain tumor types, including colon cancer, liver cancer, ovarian cancer, and pancreatic cancer85,86. GSK-3β is involved in a variety of molecular pathways, especially the PI3K/AKT pathway, to evade chemotherapy leading to MDR.

GSK-3β is generally considered a potential downstream gene product of AKT, which is a significant component of the PI3K/AKT pathway. Notably, AKT phosphorylates GSK-3β to an inactive form28. Phosphorylation of S9 and other residues mediates the activity of GSK-3β, which is inactivated by proteosome degradation. The consequence is that AKT is increased, which causes AKT to phosphorylate GSK-3β at S9 and its subsequent inactivation86,87. Activation of AKT results in decreased E-cadherin expression88. Because β-catenin also interacts with E-cadherin in the cell membrane, the loss of E-cadherin expression leads to an increase in cytoplasmic β-catenin, which may be rapidly eliminated by the GSK-3β-mediated process89. In contrast, if GSK-3β is phosphorylated or inactivated, β-catenin accumulates in the cytoplasm and transfers to the nucleus, where it interacts with cytokines, such as lymphokine active factors90. Acceleration of the transcription process and upregulation of c-Mye, c-Jun, and cyclinD1 expressions may promote tumor proliferation and increase the expression of target genes, such as MDR1 and survivin, which further leads to MDR28.

Gao et al. found that GSK-3β regulated cell viability and induced MDR via the PTEN/PI3K/AKT signaling pathway. The results indicated that GSK-3β induced PTEN phosphorylation and led to AKT activation in cancer cells. GSK-3β was a negative regulator of the PI3K/AKT signaling pathway, and GSK3β-mediate phosphorylation or PTEN inactivation resulted in depression of the PI3K/AKT signaling by macrophages/microglia91. AKT inhibited the expression of GSK-3β, which increased the expression of PTEN, and played a role in the conversion of PIP3 to PIP2, which regulated the activity of AKT via a positive feedback loop. However, the conservative mechanism of MDR in breast cancer has not been confirmed92.

In conclusion, GSK-3β exerts the dual role of drug resistance as a promoter or tumor suppressor, interacts with the PI3K/AKT pathway, regulates tumor proliferation and apoptosis, and ultimately relates to MDR.

PI3K/AKT participates in MDR processes via induction of the ABC transporter

Cancer cells exhibit MDR to anticancer drugs via several mechanisms. Obviously, one of the most major mechanisms of MDR is the overexpression of ABC superfamily transporters93. Forty-eight human ABC genes were discovered and divided into seven different subfamilies (ABCA–ABCG)94. P-gp, MRP1, and BCRP, are the three major ABC transporters that confer many of the structural diversities related to anticancer drug resistance95. A number of chemotherapeutic agents, such as paclitaxel, cisplatin, topotecan, irinotecan, and SN-38 are transported by these transporters. Recent studies indicated that the PI3K/AKT signaling pathway was involved in the formation of MDR by inducing the expression of membrane transporters96. Once activated, the PI3K/AKT pathway becomes the basis of cancer biology and enhances drug efflux by efficiently expressing ABC transporters and reducing the response of chemotherapeutic drugs, which further caused MDR29,30 (Fig. 2).

Fig. 2. The PI3K/AKT pathway regulates the gene expression of ABC transporters by activating downstream targets, such as NF-κB and Nfr2, resulting in over-expression of P-gp, MRP1, BCRP.

Fig. 2

Open in a new tab

Thus, accelerating the transport of anticancer drugs by ABC transporter to the outside that leading to MDR.

P-gp

P-gp is one of the first members of the ABC transporter. It was isolated by Juliano and Ling from colchicine-resistant Chinese hamster ovary cells97. Activation of the PI3K/AKT signaling pathway modulated the expression of P-gp, which extruded toxins and xenobiotics from cells and led to MDR23.

P-gp as a unique transporter that is expressed by two different linked genes in mouse, such as MDR1a and MDR1b, but the human MDR1 gene is responsible for P-gp production98. The PI3K/AKT kinase pathway may participate in the regulation of P-gp-mediated MDR, which is a major obstacle to the successful treatment of acute megakaryoblastic leukemia (AML)99. Previous reports showed that P-gp resisted apoptosis by transporting key caspases out of cells or inhibiting the activity of caspase. During apoptosis, cleavage occurs in a caspase-dependent manner, which indicates the complexity of the apoptosis and MDR signaling network100.

Abnormal activation of the PI3K/AKT signaling pathway mediated the expression of P-gp, which is connected to the control of the MDR phenotype23. Ganesan Muthusamy et al. found that ferulic acid contributed to the reversal of the MDR via suppression of P-gp expression and the inhibition of PI3K/AKT/NF-κB101. Xie et al. used LY294002 to inhibit the PI3K/AKT signaling pathway and showed that PI3K/AKT regulated the transcription of MDR1 in gastric cancer cells at the mRNA and protein levels102. Other studies showed that activation of the NF-κB system by the PI3K/AKT signaling pathway resulted in the increased transcription of target genes, such as ABCB1103. The AKT/NF-κB signaling pathway is the main mechanism by which CD133 regulates P-gp expression in colorectal carcinoma104. The NF-κB pathway drives MDR1 expression using the common agonist phorbol ester 12-O-tetradecanoyl-13-acetate (TPA). LY294002 treatment down regulated the expression of NF-κB, which indicates that the inhibition of the PI3K/AKT signaling pathway modulated the downregulation of MDR1 expression105,106. The modulation of P-gp phosphorylation by AKT kinase or the direct interaction of P-gp with inhibitors cannot be excluded107. In conclusion, abnormal activation of the PI3K/AKT signaling pathway mediated the expression of P-gp and led to MDR.

MRP1

MRP1 is one of the earliest described ABC transporters, and it is expressed in the plasma membrane of normal cells and cancer cells. MRP excretes physiological toxins and various agents from cells, which reduces cytotoxicity108. Study showed that inhibition of the PI3K/AKT signaling pathway led to a down regulation of MRP1 and a reversal of MDR in cancer cells109.

MRP1 is primarily expressed in physiological disorders, such as the blood–brain barrier and may limit drug uptake and retention110. Activation of the PI3K/AKT pathway promotes via MDR by regulation of the expression of a variety of cellular processes, including apoptosis (Bax/Bcl-2) and drug transport (MRP1)111, where nuclear factor erythroid-derived 2 (Nrf2) is a nuclear regulator of MRP1, and it is part of the reason for MDR. Yao et al. found that MDR was reversed by interfering with the PI3K/AKT/Nrf2 pathway, which led to MRP1-mediated drug outflow112. MRP1 inhibition depended on the inhibition of AKT phosphorylation and nuclear Nrf2. Gao et al. showed that vielanin P (VP) reduced PI3K110α. However, in the presence of siNrf2, the levels of Nrf2 and MRP1 were not further reduced. These results indicated that VP regulated MRP1 via PI3K/AKT/Nrf2. These convincing results suggested that the PI3K/AKT/Nrf2 pathway stimulated MRP1 and participated in the reversal of VP-mediated MDR cancer113. The PI3K/AKT signaling pathway inhibitor LY294002 blocked the drug transport of HT29RDB overexpressing MRP1 protein in colon cancer cells114. Therefore, the transport activities of P-gp and MRP1 may have a common regulatory mechanism involved in the PI3K/AKT pathway, and their inhibitors similarly affected resistance based on P-gp and MRP1115. In summary, aberrant activation of the PI3K/AKT signaling pathway is vital for MRP1. Inhibition of the PI3K/AKT pathway is a valid method for mediating MRP1 to reverse MDR.

BCRP

BCRP is encoded by ABCG2, which is one of the major transporters involved in xenobiotic efflux116. An increasing number of renal chemotherapeutic drugs (tyrosine kinase inhibitors) are described as BCRP substrates and can regulate their activity via the PI3K/AKT signaling pathway117,118. AKT mediates the regulation of ABCG2 function and its localization on the plasma membrane. This regulation is not a limiting factor in cancer development, but a potential response of the tumor to cancer therapy. After activated, this pathway was less sensitive to chemotherapeutic drugs, which contributed to MDR119.

Other members of the BCRP and ABCG families are substantially different from the prominent ABC transporters and are referred to as “semitransporters”120. Intracellular receptors that sense exogenous hormones and stimuli include estrogen (ER) and aryl hydrocarbons (Ahr). These stimuli trigger transcriptional responses through AKT, Kelch-like ECH-related protein 1 (KEAP1) and Nrf2, which upregulate the expression of BCRP. Upstream or downstream inhibition of epidermal growth factor receptor (EGFR/HERB)-dependent signaling regulates the AKT cascade and causes BCRP inhibition or activation. The PI3K/AKT signaling pathway regulated BCRP expression, and BCRP may protect multiple myeloma (MM) cells from the cytotoxicity of chemotherapeutic drugs by phosphatase and tensin homolog expression via a potential negative feedback loop of BCRP, and it is modulated by PTEN-mediated PI3K/AKT pathway regulation in NCI-H929 cells121. In addition, CSCs often exhibits a high ABCG2 transporter activity, the ratio of lateral cell (SP) cells in MM patients, ABCG2 expression and activation of the PI3K/AKT pathway are all positively correlated with disease progression. These studies demonstrate the key role of BCRP and PI3K/AKT pathway in controlling cancer stems and provides new strategies for the targeting of BCRP and the PI3K/AKT pathway to treat MDR.

The PI3K/AKT pathway is involved in the regulation of aerobic glycolysis to increase the energy supply leading to MDR

Metabolic reprogramming is the most important difference between cancer cells and healthy cells, which is the major metabolic phenotype of cancer122. For example, cancer cells may show additional metabolic reprogramming during chemotherapy to gain resistance to antineoplastic drugs123. The best feature of these metabolic changes is the Warburg effect, in which cancer cells tend to rely on glycolysis for energy and anabolic metabolism, even if oxygen levels are sufficient for oxidative phosphorylation. The PI3K/AKT pathway is a regulator of aerobic glycolysis and central glucose metabolism124. It confer advantages on MDR due to the acidification of the intracellular microenvironment, which may reduce drug absorption and efficiency, and/or an increase in intermediate metabolites125,126. Because cell growth depends on the biosynthesis of cellular building blocks produced by metabolic intermediates, it is easy to understand the seemingly contradictory use of this fuel127.

The PI3K/AKT signaling pathway regulates glycolysis via the upregulation of enzymes that induce stimulation of glycolytic enzymes, such as glucose transporters (GLUTs) and phosphofructokinase (PFK)128,129. GLUT1 is the main glucose transporter, and it is overexpressed in many cancer types. The upregulation of PI3K/AKT enhances its expression130. PI3K-dependent AKT direct phosphorylation and activation of PFK2 improves the production of fructose-2,6-diphosphate and ultimately activates the glycolytic rate-restricting enzyme PFK1131. The underlying mechanisms of these effects may be related to the PI3K/AKT pathway.

The PI3K/AKT pathway modulates the regulation of aerobic glycolysis in cancer cells132. The most upregulated pathway in K562/ADM cells was the PI3K/AKT signaling pathway, which was related to the reprogramming of glucose metabolism and the occurrence of MDR. The PI3K/AKT pathway was upregulated in cancer cells, which may lead to aerobic glycolysis and MDR enhancement31. The increased expression of GLUT4 confirmed the excessive activation of the PI3K/AKT/mTOR signaling pathway in cancer cells, which may enhance the ability of MDR cells to rapidly transport and consume glucose via glycolysis to produce ATP. The inhibition of glycolysis led to a depletion of ATP and led to the blockade of ATP-dependent drug outflow function that caused P-gp, which may be a strategy to reverse MDR6. Therefore, the PI3K/AKT pathway can regulate aerobic glycolysis, and it is the key energy supplier to the metabolic adaptation of cancer cells for MDR.

Prospective and conclusion

MDR is a serious obstacle to chemotherapy because it enables tumor cells to escape chemotherapy even at high doses, and it promotes tumor metastasis and recurrence. MDR is caused by multiple mechanisms. Impediments to apoptosis, reducing drug accumulation, alterations in cell cycle and enhancement of the tumor energy supply are the main reasons for MDR. Increases in DNA repair capacity, checkpoint changes, the presence of CSCs in tumors, gene mutations (PTEN mutations or disappearance, mutations in the TP53 gene), changes in drug target separation and epithelial–mesenchymal transition are also related to MDR133–135. These mechanisms must be carefully discussed in the future.

The present review showed that the PI3K/AKT pathway is a key link in the regulation of cancer MDR, and inhibition of this pathway may be an important way to solve tumor resistance. Currently PI3K/AKT inhibitors have been successfully used to enhance the sensitivity of cancer cells to drug-induced apoptosis136. For example, econazole was a new type of PI3K inhibitor, which inhibited the PI3K/AKT signaling pathway and reversed the resistance of breast cancer cells to doxorubicin137. Some studies showed that phenybutyl isoselenocyanate (ISC-4) treatment significantly inhibited PI3K/AKT activation in a dose-dependent manner, and ISC-4-mediated p-Akt inhibition resulted in primary AML (CD34+) stem cell apoptosis and enhanced the efficacy of cytidine138. Therefore, PI3K/AKT inhibitors have a central role in the development of novel strategies to overcome MDR. However, the effect of simple inhibitors in the clinical application of drugs is not ideal. Many clinical trials using PI3K, AKT, mTOR, or dual inhibitors in combination with endocrine or chemotherapy are underway. The usage of PI3K/AKT inhibitors in combination with other anti-cancer drugs requires in-depth research to provide a more effective means for clinical tumor treatment.

The present review systematically summarized that a dysfunctional PI3K/AKT pathway serves as a hub to regulate many cellular processes that are involved in MDR3 (Fig. 3). It can avoid MDR by inhibiting the PI3K/AKT pathway and related target proteins. It is possible to create an armory that overcomes MDR in different clinical situations. For example, sustained PI3K/AKT/mTORC1 activity may also be attributed to changes in miRNA expression and can induce many epigenetic changes that perpetuate MDR, which was discussed in this review. However, no therapeutics targeting specific miRNAs have made it into the clinic. miRNA inhibitors can also be expected to treat MDR by indirectly reducing the activity of the PI3K/AKT pathway. However, due to the complexity of these elements, direct targeting must be carefully studied.

Fig. 3. A dysfunctional PI3K/AKT pathway serves as a hub to regulate many cellular processes that are involved in MDR including apoptosis, ABC transporter activity, mTOR pathway, and tumor metabolism.

Fig. 3

Open in a new tab

Note: The PI3K/AKT pathway enhances drug efflux through effective expression of ABC transporter. Aerobic glycolysis is the key energy supplier to the metabolic adaptation of cancer cells for MDR. The PI3K/AKT pathway affects tumor proliferation by regulating mTOR, GSK-3β, and NF-κB. The PI3K/AKT pathway triggers XIAP to suppress the activity of caspase-3 inhibiting apoptosis. The decrease of caspase-8 will reduce migration of Bid to mitochondria, and slim down the activation of Bax and Bim, which lead to the decrease of mitochondrial membrane permeability (MOMP) and the release of cytochrome c. Caspase-8 also regulates the expression of caspase-3 to achieve cell apoptosis. The P13K/AKT/mTOR may also underlie MDR in tumor cells through inducing dysregulation of miRNA.

Acknowledgements

This work was supported by Beijing Natural Science Foundation (7202111) and National Science and Technology Major Project (2018ZX10101001-005-003) and the Fundamental Research Funds for the Central public welfare research institutes (Z0653/Z0656). All special thanks for the long-term subsidy mechanism from the Ministry of Finance and the Ministry of Education of PRC for BUCM.

Conflict of interest

The authors declare that they have no conflict of interest.

Footnotes

Edited by M. Piacentini

Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Contributor Information

Jinghong Hu, Email: hujhbj@163.com.

Cheng Lu, Email: lv_cheng0816@163.com.

Yuanyan Liu, Email: yyliu_1980@163.com.

References

  • 1.Sharma A. Chemoresistance in cancer cells: exosomes as potential regulators of therapeutic tumor heterogeneity. Nanomedicine. 2017;12:2137–2148. doi: 10.2217/nnm-2017-0184. [DOI] [PubMed] [Google Scholar]
  • 2.Garcia-Mayea Y, Mir C, Masson F, Paciucci R, LLeonart ME. Insights into new mechanisms and models of cancer stem cell multidrug resistance. Semin. Cancer Biol. 2020;60:166–180. doi: 10.1016/j.semcancer.2019.07.022. [DOI] [PubMed] [Google Scholar]
  • 3.Yang C, et al. Kanglaite reverses multidrug resistance of HCC by inducing apoptosis and cell cycle arrest via PI3K/AKT pathway. OncoTargets Ther. 2018;11:983–996. doi: 10.2147/OTT.S153814. [DOI] [PMC free article] [PubMed] [Google Scholar] [Retracted]
  • 4.Gottesman MM, Fojo T, Bates SE. Multidrug resistance in cancer: role of ATP-dependent transporters. Nat. Rev. Cancer. 2002;2:48–58. doi: 10.1038/nrc706. [DOI] [PubMed] [Google Scholar]
  • 5.DeBerardinis RJ, Lum JJ, Hatzivassiliou G, Thompson CB. The biology of cancer: metabolic reprogramming fuels cell growth and proliferation. Cell Metab. 2008;7:11–20. doi: 10.1016/j.cmet.2007.10.002. [DOI] [PubMed] [Google Scholar]
  • 6.Zhang X, et al. Glycometabolic adaptation mediates the insensitivity of drug-resistant K562/ADM leukaemia cells to adriamycin via the AKT-mTOR/c-Myc signalling pathway. Mol. Med. Rep. 2017;15:1869–1876. doi: 10.3892/mmr.2017.6189. [DOI] [PubMed] [Google Scholar]
  • 7.Soltani A, Torki S, Ghahfarokhi MS, Jami MS, Ghatrehsamani M. Targeting the phosphoinositide 3-kinase/AKT pathways by small molecules and natural compounds as a therapeutic approach for breast cancer cells. Mol. Biol. Rep. 2019;46:4809–4816. doi: 10.1007/s11033-019-04929-x. [DOI] [PubMed] [Google Scholar]
  • 8.Chen Y, et al. The critical role of PTEN/PI3K/AKT signaling pathway in shikonin-induced apoptosis and proliferation inhibition of chronic myeloid leukemia. Cell. Physiol. Biochem.l. 2018;47:981–993. doi: 10.1159/000490142. [DOI] [PubMed] [Google Scholar]
  • 9.Wu DM, et al. The PAX6-ZEB2 axis promotes metastasis and cisplatin resistance in non-small cell lung cancer through PI3K/AKT signaling. Cell Death Dis. 2019;10:349. doi: 10.1038/s41419-019-1591-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 10.Ediriweera MK, Tennekoon KH, Samarakoon SR. Role of the PI3K/AKT/mTOR signaling pathway in ovarian cancer: biological and therapeutic significance. Semin. Cancer Biol. 2019;59:147–160. doi: 10.1016/j.semcancer.2019.05.012. [DOI] [PubMed] [Google Scholar]
  • 11.Rahmani F, et al. Role of regulatory miRNAs of the PI3K/AKT/mTOR signaling in the pathogenesis of hepatocellular carcinoma. J. Cell. Physiol. 2020;235:4146–4152. doi: 10.1002/jcp.29333. [DOI] [PubMed] [Google Scholar]
  • 12.Rittler D, et al. The antitumor effect of lipophilic bisphosphonate BPH1222 in melanoma models: the role of the PI3K/Akt pathway and the small G protein Rheb. Int. J. Mol. Sci. 2019;20:4917. doi: 10.3390/ijms20194917. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 13.Ellis H, Ma CX. PI3K inhibitors in breast cancer therapy. Curr. Oncol. Rep. 2019;21:110. doi: 10.1007/s11912-019-0846-7. [DOI] [PubMed] [Google Scholar]
  • 14.Rubinstein MM, et al. Phase 2 study of LY3023414 in patients with advanced endometrial cancer harboring activating mutations in the PI3K pathway. Cancer. 2020;126:1274–1282. doi: 10.1002/cncr.32677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 15.De Marco C, et al. Multiple genetic alterations within the PI3K pathway are responsible for AKT activation in patients with ovarian carcinoma. PLoS ONE. 2013;8:e55362. doi: 10.1371/journal.pone.0055362. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 16.Harding JJ, et al. Prospective genotyping of hepatocellular carcinoma: clinical implications of next-generation sequencing for matching patients to targeted and immune therapies. Clin. Cancer Res. 2019;25:2116–2126. doi: 10.1158/1078-0432.CCR-18-2293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 17.Millis SZ, et al. Phosphatidylinositol 3-kinase pathway genomic alterations in 60,991 diverse solid tumors informs targeted therapy opportunities. Cancer. 2019;125:1185–1199. doi: 10.1002/cncr.31921. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 18.Wang DG, et al. Anti-tumor activity of the X-linked inhibitor of apoptosis (XIAP) inhibitor embelin in gastric cancer cells. Mol. Cell. Biochem. 2014;386:143–152. doi: 10.1007/s11010-013-1853-x. [DOI] [PubMed] [Google Scholar]
  • 19.Jin J, Xiong Y, Cen B. Bcl-2 and Bcl-xL mediate resistance to receptor tyrosine kinase-targeted therapy in lung and gastric cancer. Anti-cancer Drugs. 2017;28:1141–1149. doi: 10.1097/CAD.0000000000000561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 20.Rocha Gda G, Oliveira RR, Kaplan MA, Gattass CR. 3beta-acetyl tormentic acid reverts MRP1/ABCC1 mediated cancer resistance through modulation of intracellular levels of GSH and inhibition of GST activity. Eur. J. Pharmacol. 2014;741:140–149. doi: 10.1016/j.ejphar.2014.07.054. [DOI] [PubMed] [Google Scholar]
  • 21.Tasioudi KE, et al. Immunohistochemical and molecular analysis of PI3K/AKT/mTOR pathway in esophageal carcinoma. Acta Pathol. Microbiol. Immunol. Scand. 2015;123:639–647. doi: 10.1111/apm.12398. [DOI] [PubMed] [Google Scholar]
  • 22.House CD, et al. NFkappaB promotes ovarian tumorigenesis via classical pathways that support proliferative cancer cells and alternative pathways that support ALDH(+) cancer stem-like cells. Cancer Res. 2017;77:6927–6940. doi: 10.1158/0008-5472.CAN-17-0366. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 23.Sui H, et al. Zuo Jin Wan reverses P-gp-mediated drug-resistance by inhibiting activation of the PI3K/Akt/NF-kappaB pathway. BMC Complement. Altern. Med. 2014;14:279. doi: 10.1186/1472-6882-14-279. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 24.Zhu DD, et al. Significance of NF-kappaB activation in immortalization of nasopharyngeal epithelial cells. Int. J. Cancer. 2016;138:1175–1185. doi: 10.1002/ijc.29850. [DOI] [PubMed] [Google Scholar]
  • 25.Kuznetsov VA, Tang Z, Ivshina AV. Identification of common oncogenic and early developmental pathways in the ovarian carcinomas controlling by distinct prognostically significant microRNA subsets. BMC Genom. 2017;18:692. doi: 10.1186/s12864-017-4027-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 26.Wu SJ, Chen J, Wu B, Wang YJ, Guo KY. MicroRNA-150 enhances radiosensitivity by inhibiting the AKT pathway in NK/T cell lymphoma. J. Exp. Clin. Cancer Res. 2018;37:18. doi: 10.1186/s13046-017-0639-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 27.Kyrochristos ID, Ziogas DE, Roukos DH. Dynamic genome and transcriptional network-based biomarkers and drugs: precision in breast cancer therapy. Med. Res. Rev. 2019;39:1205–1227. doi: 10.1002/med.21549. [DOI] [PubMed] [Google Scholar]
  • 28.Oh S, Kim H, Nam K, Shin I. Silencing of Glut1 induces chemoresistance via modulation of Akt/GSK-3beta/beta-catenin/survivin signaling pathway in breast cancer cells. Arch. Biochem. Biophys. 2017;636:110–122. doi: 10.1016/j.abb.2017.08.009. [DOI] [PubMed] [Google Scholar]
  • 29.Lampada A, et al. mTORC1-independent autophagy regulates receptor tyrosine kinase phosphorylation in colorectal cancer cells via an mTORC2-mediated mechanism. Cell Death Differ. 2017;24:1045–1062. doi: 10.1038/cdd.2017.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 30.Tazzari PL, et al. Multidrug resistance-associated protein 1 expression is under the control of the phosphoinositide 3 kinase/Akt signal transduction network in human acute myelogenous leukemia blasts. Leukemia. 2007;21:427–438. doi: 10.1038/sj.leu.2404523. [DOI] [PubMed] [Google Scholar]
  • 31.Zhang X, et al. Targeting glycometabolic reprogramming to restore the sensitivity of leukemia drug-resistant K562/ADM cells to adriamycin. Life Sci. 2018;215:1–10. doi: 10.1016/j.lfs.2018.10.050. [DOI] [PubMed] [Google Scholar]
  • 32.Shariati M, Meric-Bernstam F. Targeting AKT for cancer therapy. Expert Opin. Investig. Drugs. 2019;28:977–988. doi: 10.1080/13543784.2019.1676726. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 33.Yamamoto T, et al. Reduced methylation of PFKFB3 in cancer cells shunts glucose towards the pentose phosphate pathway. Nat. Commun. 2014;5:3480. doi: 10.1038/ncomms4480. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 34.Fischer B, Frei C, Moura U, Stahel R, Felley-Bosco E. Inhibition of phosphoinositide-3 kinase pathway down regulates ABCG2 function and sensitizes malignant pleural mesothelioma to chemotherapy. Lung Cancer. 2012;78:23–29. doi: 10.1016/j.lungcan.2012.07.005. [DOI] [PubMed] [Google Scholar]
  • 35.Liu B, Wang C, Chen P, Cheng B, Cheng Y. RACKI induces chemotherapy resistance in esophageal carcinoma by upregulating the PI3K/AKT pathway and Bcl-2 expression. OncoTargets Ther. 2018;11:211–220. doi: 10.2147/OTT.S152818. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 36.Sochalska M, Tuzlak S, Egle A, Villunger A. Lessons from gain- and loss-of-function models of pro-survival Bcl2 family proteins: implications for targeted therapy. FEBS J. 2015;282:834–849. doi: 10.1111/febs.13188. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 37.Zhang YH, Wu YL, Tashiro S, Onodera S, Ikejima T. Reactive oxygen species contribute to oridonin-induced apoptosis and autophagy in human cervical carcinoma HeLa cells. Acta Pharmacol. Sin. 2011;32:1266–1275. doi: 10.1038/aps.2011.92. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 38.Goan, Y. G., Wu, W. T., Liu, C. I., Neoh, C. A. & Wu, Y. J. Involvement of mitochondrial dysfunction, endoplasmic reticulum stress, and the PI3K/AKT/mTOR pathway in nobiletin-induced apoptosis of human bladder cancer cells. Molecules.10.3390/molecules24162881 (2019). [DOI] [PMC free article] [PubMed]
  • 39.Volkmann N, Marassi FM, Newmeyer DD, Hanein D. The rheostat in the membrane: BCL-2 family proteins and apoptosis. Cell Death Differ. 2014;21:206–215. doi: 10.1038/cdd.2013.153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 40.Choi E, et al. AKT1-targeted proapoptotic activity of compound K in human breast cancer cells. J. Ginseng Res. 2019;43:692–698. doi: 10.1016/j.jgr.2019.07.001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 41.Adams JM, Cory S. The BCL-2 arbiters of apoptosis and their growing role as cancer targets. Cell Death Differ. 2018;25:27–36. doi: 10.1038/cdd.2017.161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 42.Li P, et al. Nicotinic agonist inhibits cardiomyocyte apoptosis in CVB3-induced myocarditis via alpha3beta4-nAChR/PI3K/Akt-dependent survivin upregulation. Oxid. Med. Cell. Longev. 2019;2019:9496419. doi: 10.1155/2019/9496419. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 43.Heavey S, O’Byrne KJ, Gately K. Strategies for co-targeting the PI3K/AKT/mTOR pathway in NSCLC. Cancer Treat. Rev. 2014;40:445–456. doi: 10.1016/j.ctrv.2013.08.006. [DOI] [PubMed] [Google Scholar]
  • 44.Garcia-Regalado A, Vargas M, Garcia-Carranca A, Arechaga-Ocampo E, Gonzalez-De la Rosa CH. Activation of Akt pathway by transcription-independent mechanisms of retinoic acid promotes survival and invasion in lung cancer cells. Mol. Cancer. 2013;12:44. doi: 10.1186/1476-4598-12-44. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 45.Geng HX, et al. 14,15-EET suppresses neuronal apoptosis in ischemia-reperfusion through the mitochondrial pathway. Neurochem. Res. 2017;42:2841–2849. doi: 10.1007/s11064-017-2297-6. [DOI] [PubMed] [Google Scholar]
  • 46.Wang JH, Nao JF, Zhang M, He P. 20(s)-ginsenoside Rg3 promotes apoptosis in human ovarian cancer HO-8910 cells through PI3K/Akt and XIAP pathways. Tumour Biol. 2014;35:11985–11994. doi: 10.1007/s13277-014-2497-5. [DOI] [PubMed] [Google Scholar]
  • 47.Fei B, Chi AL, Weng Y. Hydroxycamptothecin induces apoptosis and inhibits tumor growth in colon cancer by the downregulation of survivin and XIAP expression. World J. Surg. Oncol. 2013;11:120. doi: 10.1186/1477-7819-11-120. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 48.Fraser M, et al. p53 is a determinant of X-linked inhibitor of apoptosis protein/Akt-mediated chemoresistance in human ovarian cancer cells. Cancer Res. 2003;63:7081–7088. [PubMed] [Google Scholar]
  • 49.Cheng JQ, et al. Role of X-linked inhibitor of apoptosis protein in chemoresistance in ovarian cancer: possible involvement of the phosphoinositide-3 kinase/Akt pathway. Drug Resist. Updates. 2002;5:131–146. doi: 10.1016/S1368-7646(02)00003-1. [DOI] [PubMed] [Google Scholar]
  • 50.Kahana S, et al. Proteasome inhibitors sensitize glioma cells and glioma stem cells to TRAIL-induced apoptosis by PKCepsilon-dependent downregulation of AKT and XIAP expressions. Cell. Signal. 2011;23:1348–1357. doi: 10.1016/j.cellsig.2011.03.017. [DOI] [PubMed] [Google Scholar]
  • 51.Kaufmann T, Strasser A, Jost PJ. Fas death receptor signalling: roles of Bid and XIAP. Cell Death Differ. 2012;19:42–50. doi: 10.1038/cdd.2011.121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 52.Balaji S, et al. NF-kappaB signaling and its relevance to the treatment of mantle cell lymphoma. J. Hematol. Oncol. 2018;11:83. doi: 10.1186/s13045-018-0621-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 53.Qian H, et al. X-linked inhibitor of apoptosis protein inhibitor Embelin induces apoptosis via PI3K/Akt pathway and inhibits invasion in osteosarcoma cells. J. Cancer Res. therapeutics. 2018;14:S648–S655. doi: 10.4103/0973-1482.203599. [DOI] [PubMed] [Google Scholar]
  • 54.Ali, R. et al. ATM regulated PTEN degradation Is XIAP E3 ubiquitin ligase mediated in p85alpha deficient cancer cells and influence platinum sensitivity. Cells.10.3390/cells8101271 (2019). [DOI] [PMC free article] [PubMed]
  • 55.Asselin E, Mills GB, Tsang BK. XIAP regulates Akt activity and caspase-3-dependent cleavage during cisplatin-induced apoptosis in human ovarian epithelial cancer cells. Cancer Res. 2001;61:1862–1868. [PubMed] [Google Scholar]
  • 56.Dan HC, et al. Akt phosphorylation and stabilization of X-linked inhibitor of apoptosis protein (XIAP) J. Biol. Chem. 2016;291:22846. doi: 10.1074/jbc.A116.312044. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 57.Huang X, Wu Z, Mei Y, Wu M. XIAP inhibits autophagy via XIAP-Mdm2-p53 signalling. EMBO J. 2013;32:2204–2216. doi: 10.1038/emboj.2013.133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 58.Li X, et al. Down-regulated lncRNA SLC25A5-AS1 facilitates cell growth and inhibits apoptosis via miR-19a-3p/PTEN/PI3K/AKT signalling pathway in gastric cancer. J. Cell. Mol. Med. 2019;23:2920–2932. doi: 10.1111/jcmm.14200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 59.Xu S, et al. Metformin suppresses tumor progression by inactivating stromal fibroblasts in ovarian cancer. Mol. Cancer Ther. 2018;17:1291–1302. doi: 10.1158/1535-7163.MCT-17-0927. [DOI] [PubMed] [Google Scholar]
  • 60.Ghandadi M, et al. Wnt-beta-catenin signaling pathway, the Achilles’ heels of cancer multidrug resistance. Curr. Pharm. Des. 2019;25:4192–4207. doi: 10.2174/1381612825666191112142943. [DOI] [PubMed] [Google Scholar]
  • 61.Zhang, P., Chen, X. B., Ding, B. Q., Liu, H. L. & He, T. Down-regulation of ABCE1 inhibits temozolomide resistance in glioma through the PI3K/Akt/NF-kappaB signaling pathway. Biosci. Rep.10.1042/BSR20181711 (2018). [DOI] [PMC free article] [PubMed]
  • 62.Usman MW, et al. Macrophages confer resistance to PI3K inhibitor GDC-0941 in breast cancer through the activation of NF-kappaB signaling. Cell Death Dis. 2018;9:809. doi: 10.1038/s41419-018-0849-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 63.Yasuda T. Hyaluronan inhibits Akt, leading to nuclear factor-kappaB down-regulation in lipopolysaccharide-stimulated U937 macrophages. J. Pharmacol. Sci. 2011;115:509–515. doi: 10.1254/jphs.10244FP. [DOI] [PubMed] [Google Scholar]
  • 64.Chen P, et al. Luteolin inhibits cell proliferation and induces cell apoptosis via down-regulation of mitochondrial membrane potential in esophageal carcinoma cells EC1 and KYSE450. Oncotarget. 2017;8:27471–27480. doi: 10.18632/oncotarget.15832. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 65.Brown KK, Toker A. The phosphoinositide 3-kinase pathway and therapy resistance in cancer. F1000prime Rep. 2015;7:13. doi: 10.12703/P7-13. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 66.Dutton A, Reynolds GM, Dawson CW, Young LS, Murray PG. Constitutive activation of phosphatidyl-inositide 3 kinase contributes to the survival of Hodgkin’s lymphoma cells through a mechanism involving Akt kinase and mTOR. J. Pathol. 2005;205:498–506. doi: 10.1002/path.1725. [DOI] [PubMed] [Google Scholar]
  • 67.Hu Y, et al. Effects of PI3K inhibitor NVP-BKM120 on overcoming drug resistance and eliminating cancer stem cells in human breast cancer cells. Cell Death Dis. 2015;6:e2020. doi: 10.1038/cddis.2015.363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 68.Eberle, J. Countering TRAIL resistance in melanoma. Cancers.10.3390/cancers11050656 (2019). [DOI] [PMC free article] [PubMed]
  • 69.Ni Z, Yi J. Oxymatrine induces nasopharyngeal cancer cell death through inhibition of PI3K/AKT and NFkappaB pathways. Mol. Med. Rep. 2017;16:9701–9706. doi: 10.3892/mmr.2017.7822. [DOI] [PubMed] [Google Scholar]
  • 70.Chen W, Liu X, Yuan S, Qiao T. HSPA12B overexpression induces cisplatin resistance in non-small-cell lung cancer by regulating the PI3K/Akt/NF-kappaB signaling pathway. Oncol. Lett. 2018;15:3883–3889. doi: 10.3892/ol.2018.7800. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 71.Brotelle T, Bay JO. PI3K-AKT-mTOR pathway: description, therapeutic development, resistance, predictive/prognostic biomarkers and therapeutic applications for cancer. Bull. Cancer. 2016;103:18–29. doi: 10.1016/j.bulcan.2015.09.011. [DOI] [PubMed] [Google Scholar]
  • 72.Tong X, Pelling JC. Targeting the PI3K/Akt/mTOR axis by apigenin for cancer prevention. Anti-cancer Agents Med. Chem. 2013;13:971–978. doi: 10.2174/18715206113139990119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 73.Deng L, et al. The PI3K/mTOR dual inhibitor BEZ235 suppresses proliferation and migration and reverses multidrug resistance in acute myeloid leukemia. Acta Pharmacol. Sin. 2017;38:382–391. doi: 10.1038/aps.2016.121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 74.Mabuchi S, Kuroda H, Takahashi R, Sasano T. The PI3K/AKT/mTOR pathway as a therapeutic target in ovarian cancer. Gynecol. Oncol. 2015;137:173–179. doi: 10.1016/j.ygyno.2015.02.003. [DOI] [PubMed] [Google Scholar]
  • 75.Kumar V, et al. Therapeutic suppression of mTOR (mammalian target of rapamycin) signaling prevents and reverses salt-induced hypertension and kidney injury in dahl salt-sensitive rats. Hypertension. 2019;73:630–639. doi: 10.1161/HYPERTENSIONAHA.118.12378. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 76.Dai Y, et al. lncRNA MEG3 suppresses the tumorigenesis of hemangioma by sponging miR-494 and regulating PTEN/ PI3K/AKT pathway. Cell. Physiol. Biochem. 2018;51:2872–2886. doi: 10.1159/000496040. [DOI] [PubMed] [Google Scholar]
  • 77.Liu P, et al. PtdIns(3,4,5)P3-dependent activation of the mTORC2 kinase complex. Cancer Discov. 2015;5:1194–1209. doi: 10.1158/2159-8290.CD-15-0460. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 78.Yang G, Murashige DS, Humphrey SJ, James DE. A positive feedback loop between Akt and mTORC2 via SIN1 phosphorylation. Cell Rep. 2015;12:937–943. doi: 10.1016/j.celrep.2015.07.016. [DOI] [PubMed] [Google Scholar]
  • 79.Mercer PF, et al. Exploration of a potent PI3 kinase/mTOR inhibitor as a novel anti-fibrotic agent in IPF. Thorax. 2016;71:701–711. doi: 10.1136/thoraxjnl-2015-207429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 80.Yu X, Zheng H, Chan MT, Wu WK. Modulation of chemoresponsiveness to platinum-based agents by microRNAs in cancer. Am. J. Cancer Res. 2017;7:1769–1778. [PMC free article] [PubMed] [Google Scholar]
  • 81.Yue D, Qin X. miR-182 regulates trastuzumab resistance by targeting MET in breast cancer cells. Cancer Gene Ther. 2019;26:1–10. doi: 10.1038/s41417-018-0031-4. [DOI] [PubMed] [Google Scholar]
  • 82.Zeng L, et al. Long non-coding RNA XLOC_006753 promotes the development of multidrug resistance in gastric cancer cells through the PI3K/AKT/mTOR signaling pathway. Cell. Physiol. Biochem. 2018;51:1221–1236. doi: 10.1159/000495499. [DOI] [PubMed] [Google Scholar]
  • 83.Li Z, Qian J, Li J, Zhu C. Knockdown of lncRNA-HOTAIR downregulates the drug-resistance of breast cancer cells to doxorubicin via the PI3K/AKT/mTOR signaling pathway. Exp. Ther. Med. 2019;18:435–442. doi: 10.3892/etm.2019.7629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 84.Patel S, Macaulay K, Woodgett JR. Tissue-specific analysis of glycogen synthase kinase-3alpha (GSK-3alpha) in glucose metabolism: effect of strain variation. PLoS ONE. 2011;6:e15845. doi: 10.1371/journal.pone.0015845. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 85.Wang W, et al. NOR1 suppresses cancer stem-like cells properties of tumor cells via the inhibition of the AKT-GSK-3beta-Wnt/beta-catenin-ALDH1A1 signal circuit. J. Cell. Physiol. 2017;232:2829–2840. doi: 10.1002/jcp.25706. [DOI] [PubMed] [Google Scholar]
  • 86.von Achenbach C, et al. Synergistic growth inhibition mediated by dual PI3K/mTOR pathway targeting and genetic or direct pharmacological AKT inhibition in human glioblastoma models. J. Neurochem. 2020;153:510–524. doi: 10.1111/jnc.14899. [DOI] [PubMed] [Google Scholar]
  • 87.Zhao ZA, et al. Adenosine A2A receptor inactivation alleviates early-onset cognitive dysfunction after traumatic brain injury involving an inhibition of tau hyperphosphorylation. Transl. Psychiatry. 2017;7:e1123. doi: 10.1038/tp.2017.98. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 88.Zhou Q, et al. Luteolin inhibits invasion of prostate cancer PC3 cells through E-cadherin. Mol. Cancer Ther. 2009;8:1684–1691. doi: 10.1158/1535-7163.MCT-09-0191. [DOI] [PubMed] [Google Scholar]
  • 89.Huber AH, Weis WI. The structure of the beta-catenin/E-cadherin complex and the molecular basis of diverse ligand recognition by beta-catenin. Cell. 2001;105:391–402. doi: 10.1016/s0092-8674(01)00330-0. [DOI] [PubMed] [Google Scholar]
  • 90.Ram Makena, M. et al. Wnt/beta-catenin signaling: the culprit in pancreatic carcinogenesis and therapeutic resistance. Int. J. Mol. Sci.10.3390/ijms20174242 (2019). [DOI] [PMC free article] [PubMed]
  • 91.Wang G, et al. HDAC inhibition prevents white matter injury by modulating microglia/macrophage polarization through the GSK3beta/PTEN/Akt axis. Proc. Natl Acad. Sci. USA. 2015;112:2853–2858. doi: 10.1073/pnas.1501441112. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 92.Li, B., Yuan, Z., Jiang, J. & Rao, Y. Anti-tumor activity of Shikonin against afatinib resistant non-small cell lung cancer via negative regulation of PI3K/Akt signaling pathway. Biosci. Rep.10.1042/BSR20181693 (2018). [DOI] [PMC free article] [PubMed]
  • 93.Hasanovic, A. & Mus-Veteau, I. Targeting the multidrug transporter Ptch1 potentiates chemotherapy efficiency. Cells.10.3390/cells7080107 (2018). [DOI] [PMC free article] [PubMed]
  • 94.Zhao RQ, et al. Y6, an epigallocatechin gallate derivative, reverses ABCG2-mediated mitoxantrone resistance. Front. Pharmacol. 2018;9:1545. doi: 10.3389/fphar.2018.01545. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 95.Ranjbar S, et al. 5-Oxo-hexahydroquinoline derivatives as modulators of P-gp, MRP1 and BCRP transporters to overcome multidrug resistance in cancer cells. Toxicol. Appl. Pharmacol. 2019;362:136–149. doi: 10.1016/j.taap.2018.10.025. [DOI] [PubMed] [Google Scholar]
  • 96.Guo Q, et al. Ubenimex reverses MDR in gastric cancer cells by activating caspase-3-mediated apoptosis and suppressing the expression of membrane transport proteins. BioMed. Res. Int. 2019;2019:4390839. doi: 10.1155/2019/4390839. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 97.Juliano RL, Ling V. A surface glycoprotein modulating drug permeability in Chinese hamster ovary cell mutants. Biochim. Biophys. Acta. 1976;455:152–162. doi: 10.1016/0005-2736(76)90160-7. [DOI] [PubMed] [Google Scholar]
  • 98.Levy ES, et al. Reversible inhibition of efflux transporters by hydrogel microdevices. Eur. J. Pharm. Biopharm. 2019;145:76–84. doi: 10.1016/j.ejpb.2019.10.007. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 99.Hashimoto N, et al. Metabolome analysis reveals dermal histamine accumulation in murine dermatitis provoked by genetic deletion of P-glycoprotein and breast cancer resistance protein. Pharm. Res. 2019;36:158. doi: 10.1007/s11095-019-2695-3. [DOI] [PubMed] [Google Scholar]
  • 100.Teng, Y. N., Wang, Y. H., Wu, T. S., Hung, H. Y. & Hung, C. C. Zhankuic acids A, B and C from Taiwanofungus camphoratus act as cytotoxicity enhancers by regulating P-glycoprotein in multi-drug resistant cancer cells. Biomolecules.10.3390/biom9120759 (2019). [DOI] [PMC free article] [PubMed]
  • 101.Muthusamy G, Gunaseelan S, Prasad NR. Ferulic acid reverses P-glycoprotein-mediated multidrug resistance via inhibition of PI3K/Akt/NF-kappaB signaling pathway. J. Nutr. Biochem. 2019;63:62–71. doi: 10.1016/j.jnutbio.2018.09.022. [DOI] [PubMed] [Google Scholar]
  • 102.Xie X, Tang B, Zhou J, Gao Q, Zhang P. Inhibition of the PI3K/Akt pathway increases the chemosensitivity of gastric cancer to vincristine. Oncol. Rep. 2013;30:773–782. doi: 10.3892/or.2013.2520. [DOI] [PubMed] [Google Scholar]
  • 103.Kaewpiboon C, et al. Feroniellin A-induced autophagy causes apoptosis in multidrug-resistant human A549 lung cancer cells. Int. J. Oncol. 2014;44:1233–1242. doi: 10.3892/ijo.2014.2297. [DOI] [PubMed] [Google Scholar]
  • 104.Yuan Z, et al. Targeting CD133 reverses drug-resistance via the AKT/NF-kappaB/MDR1 pathway in colorectal cancer. Br. J. Cancer. 2020;122:1342–1353. doi: 10.1038/s41416-020-0783-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 105.Shahbazi R, et al. Hesperidin inhibits insulin-induced phosphoinositide 3-kinase/Akt activation in human pre-B cell line NALM-6. J. Cancer Res. Ther. 2018;14:503–508. doi: 10.4103/0973-1482.157323. [DOI] [PubMed] [Google Scholar]
  • 106.Cai H, Li L, Jiang J, Zhao C, Yang C. Costunolide enhances sensitivity of K562/ADR chronic myeloid leukemia cells to doxorubicin through PI3K/Akt pathway. Phytother. Res. 2019;33:1683–1688. doi: 10.1002/ptr.6355. [DOI] [PubMed] [Google Scholar]
  • 107.Li Y, et al. Guajadial reverses multidrug resistance by inhibiting ABC transporter expression and suppressing the PI3K/Akt pathway in drug-resistant breast cancer cells. Chem.-Biol. Interact. 2019;305:98–104. doi: 10.1016/j.cbi.2019.03.032. [DOI] [PubMed] [Google Scholar]
  • 108.Roundhill EA, Burchill SA. Detection and characterisation of multi-drug resistance protein 1 (MRP-1) in human mitochondria. Br. J. Cancer. 2012;106:1224–1233. doi: 10.1038/bjc.2012.40. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 109.Tripathi SC, et al. MCAM mediates chemoresistance in small-cell lung cancer via the PI3K/AKT/SOX2 signaling pathway. Cancer Res. 2017;77:4414–4425. doi: 10.1158/0008-5472.CAN-16-2874. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 110.Li S, et al. The roles of breast cancer resistance protein (BCRP/ABCG2) and multidrug resistance-associated proteins (MRPs/ABCCs) in the excretion of cycloicaritin-3-O-glucoronide in UGT1A1-overexpressing HeLa cells. Chem.-Biol. Interact. 2018;296:45–56. doi: 10.1016/j.cbi.2018.09.001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 111.Abdul-Ghani R, et al. The PI3K inhibitor LY294002 blocks drug export from resistant colon carcinoma cells overexpressing MRP1. Oncogene. 2006;25:1743–1752. doi: 10.1038/sj.onc.1209201. [DOI] [PubMed] [Google Scholar]
  • 112.Yao J, Wei X, Lu Y. Chaetominine reduces MRP1-mediated drug resistance via inhibiting PI3K/Akt/Nrf2 signaling pathway in K562/Adr human leukemia cells. Biochem. Biophys. Res. Commun. 2016;473:867–873. doi: 10.1016/j.bbrc.2016.03.141. [DOI] [PubMed] [Google Scholar]
  • 113.Gao HL, Xia YZ, Zhang YL, Yang L, Kong LY. Vielanin P enhances the cytotoxicity of doxorubicin via the inhibition of PI3K/Nrf2-stimulated MRP1 expression in MCF-7 and K562 DOX-resistant cell lines. Phytomedicine. 2019;58:152885. doi: 10.1016/j.phymed.2019.152885. [DOI] [PubMed] [Google Scholar]
  • 114.Li Y, et al. Involvement of the PI3K/Akt/Nrf2 signaling pathway in resveratrol-mediated reversal of drug resistance in HL-60/ADR cells. Nutr. Cancer. 2019;71:1007–1018. doi: 10.1080/01635581.2019.1578387. [DOI] [PubMed] [Google Scholar]
  • 115.To KK, Tomlinson B. Targeting the ABCG2-overexpressing multidrug resistant (MDR) cancer cells by PPARgamma agonists. Br. J. Pharmacol. 2013;170:1137–1151. doi: 10.1111/bph.12367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 116.Li H, et al. Comparison of LC–MS/MS-based targeted proteomics and conventional analytical methods for monitoring breast cancer resistance protein expression. Life Sci. 2019;231:116548. doi: 10.1016/j.lfs.2019.116548. [DOI] [PubMed] [Google Scholar]
  • 117.Feng S, et al. Proton pump inhibitor pantoprazole inhibits the proliferation, selfrenewal and chemoresistance of gastric cancer stem cells via the EMT/betacatenin pathways. Oncol. Rep. 2016;36:3207–3214. doi: 10.3892/or.2016.5154. [DOI] [PubMed] [Google Scholar]
  • 118.Chen Z, et al. Suppression of ABCG2 inhibits cancer cell proliferation. Int. J. Cancer. 2010;126:841–851. doi: 10.1002/ijc.24796. [DOI] [PubMed] [Google Scholar]
  • 119.Lin J, et al. Scutellaria barbata D. Don inhibits 5-fluorouracil resistance in colorectal cancer by regulating PI3K/AKT pathway. Oncol. Rep. 2017;38:2293–2300. doi: 10.3892/or.2017.5892. [DOI] [PubMed] [Google Scholar]
  • 120.Shafran A, et al. ABCG2 harboring the Gly482 mutation confers high-level resistance to various hydrophilic antifolates. Cancer Res. 2005;65:8414–8422. doi: 10.1158/0008-5472.CAN-04-4547. [DOI] [PubMed] [Google Scholar]
  • 121.Wang L, Lin N, Li Y. The PI3K/AKT signaling pathway regulates ABCG2 expression and confers resistance to chemotherapy in human multiple myeloma. Oncol. Rep. 2019;41:1678–1690. doi: 10.3892/or.2019.6968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 122.Yeh YC, et al. AKT1 internal tandem duplications and point mutations are the genetic hallmarks of sclerosing pneumocytoma. Modern Pathol. 2020;33:391–403. doi: 10.1038/s41379-019-0357-y. [DOI] [PubMed] [Google Scholar]
  • 123.Lopes-Rodrigues V, et al. Identification of the metabolic alterations associated with the multidrug resistant phenotype in cancer and their intercellular transfer mediated by extracellular vesicles. Sci. Rep. 2017;7:44541. doi: 10.1038/srep44541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 124.Robey RB, Hay N. Is Akt the “Warburg kinase”?-Akt-energy metabolism interactions and oncogenesis. Semin. Cancer Biol. 2009;19:25–31. doi: 10.1016/j.semcancer.2008.11.010. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 125.Ayuk, S. M. & Abrahamse, H. mTOR Signaling Pathway in Cancer Targets Photodynamic Therapy In Vitro. Cells. 10.3390/cells8050431 (2019). [DOI] [PMC free article] [PubMed]
  • 126.Hosios AM, et al. Amino acids rather than glucose account for the majority of cell mass in proliferating mammalian cells. Dev. Cell. 2016;36:540–549. doi: 10.1016/j.devcel.2016.02.012. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 127.Knoechel B, Aster JC. Metabolic mechanisms of drug resistance in leukemia. Cell Metab. 2015;22:759–760. doi: 10.1016/j.cmet.2015.10.005. [DOI] [PubMed] [Google Scholar]
  • 128.Zhu W, et al. Dihydroartemisinin suppresses glycolysis of LNCaP cells by inhibiting PI3K/AKT pathway and downregulating HIF-1alpha expression. Life Sci. 2019;233:116730. doi: 10.1016/j.lfs.2019.116730. [DOI] [PubMed] [Google Scholar]
  • 129.Ersahin T, Tuncbag N, Cetin-Atalay R. The PI3K/AKT/mTOR interactive pathway. Mol. Biosyst. 2015;11:1946–1954. doi: 10.1039/c5mb00101c. [DOI] [PubMed] [Google Scholar]
  • 130.Feng C, et al. SHBG expression is correlated with PI3K/AKT pathway activity in a cellular model of human insulin resistance. Gynecol. Endocrinol. 2018;34:567–573. doi: 10.1080/09513590.2017.1411474. [DOI] [PubMed] [Google Scholar]
  • 131.Lee JH, et al. EGFR-phosphorylated platelet isoform of phosphofructokinase 1 promotes PI3K activation. Mol. Cell. 2018;70:197–210. e197. doi: 10.1016/j.molcel.2018.03.018. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 132.Pollen AA, et al. Establishing cerebral organoids as models of human-specific brain evolution. Cell. 2019;176:743–756. e717. doi: 10.1016/j.cell.2019.01.017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 133.Marzagalli M, et al. Cellular and molecular biology of cancer stem cells in melanoma: Possible therapeutic implications. Semin. Cancer Biol. 2019;59:221–235. doi: 10.1016/j.semcancer.2019.06.019. [DOI] [PubMed] [Google Scholar]
  • 134.Liu L, et al. Transgelin 2 promotes paclitaxel resistance, migration, and invasion of breast cancer by directly interacting with PTEN and activating PI3K/Akt/GSK-3beta pathway. Mol. Cancer Ther. 2019;18:2457–2468. doi: 10.1158/1535-7163.MCT-19-0261. [DOI] [PubMed] [Google Scholar]
  • 135.Bukowski, K., Kciuk, M. & Kontek, R. Mechanisms of multidrug resistance in cancer chemotherapy. Int. J. Mol. Sci.10.3390/ijms21093233 (2020). [DOI] [PMC free article] [PubMed]
  • 136.Meng Y, et al. Ursolic acid induces apoptosis of prostate cancer cells via the PI3K/Akt/mTOR pathway. Am. J. Chin. Med. 2015;43:1471–1486. doi: 10.1142/S0192415X15500834. [DOI] [PubMed] [Google Scholar]
  • 137.Dong C, et al. Econazole nitrate reversed the resistance of breast cancer cells to Adriamycin through inhibiting the PI3K/AKT signaling pathway. Am. J. Cancer Res. 2020;10:263–274. [PMC free article] [PubMed] [Google Scholar]
  • 138.Annageldiyev C, et al. The PI3K/AKT pathway inhibitor ISC-4 induces apoptosis and inhibits growth of leukemia in preclinical models of acute myeloid leukemia. Front. Oncol. 2020;10:393. doi: 10.3389/fonc.2020.00393. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Cell Death & Disease are provided here courtesy of Nature Publishing Group

RESOURCES