Figure 1.

Transcriptional Changes in the Posterior Mediobasal Hypothalamus (MBHp) in Response to Exogenous Leptin

(A) Illustration of experimental groups. Mice were divided into three groups (n = 4/group): wild-type diestrous treated with intraperitoneal (i.p.) saline (WT) and leptin-deficient females treated with i.p. saline (Lep^ob) or i.p. leptin (Lep^ob + leptin). After 4 injections of leptin, twice daily, puberty onset was observed. Mice were euthanized 1 h after the last saline or leptin injection. The MBHp was micro-dissected and collected for RNA-seq.

(B) Hierarchical clustering of differentially expressed genes (DEGs) across groups

(C) Venn diagram showing number of DEGs comparing groups.

(D) Recovered DEGs (rDEGs) associated with reproduction and/or development processes (gene ontology/GO terms 0000003 and 0032502, respectively). “rDEGs undefined” are selected genes whose expression was not recovered when PMv and Arc were individually analyzed.

(E) rDEGs are genes with recovered expression in PMv or Arc following leptin administration.

(F) “rDEGs undefined” are selected genes whose expression was not recovered when PMv and Arc were individually analyzed.

One-way ANOVA followed by Tukey's test were used. a, b, and c: different superscripts indicate p < 0.05. Scale bar: 400 μm. See also Figure S1 and Table S1.