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. 2020 Sep 29;10:16375. doi: 10.1038/s41598-020-71156-x

Author Correction: Pivotal role of CD103 in the development of psoriasiform dermatitis

Takehito Fukui 1,2, Tomohiro Fukaya 1,7, Tomofumi Uto 1,7, Hideaki Takagi 1,7, Junta Nasu 1,2, Noriaki Miyanaga 1,3, Yotaro Nishikawa 1,4, Haruhiko Koseki 5, Narantsog Choijookhuu 6, Yoshitaka Hishikawa 6, Yoshihiro Yamashita 2, Katsuaki Sato 1,7,
PMCID: PMC7522241  PMID: 32989237

Correction to: Scientific Reports 10.1038/s41598-020-65355-9, published online 20 May 2020

This Article contains errors in the Methods section, under the subheading ‘Generation of Cd103−/− mice’,

“The linearized targeting construct was introduced by electroporation into C57BL/6-derived JN/2 recombinant embryonic stem cell (ESC) and neomycin-resistant clones were first screened for homologous recombination by PCR utilizing a pair of the following oligonucleotides: Primer 1 (5′-ATA TGT AGT GTC TGG TCA GGA TAA TAG TTG-3′) and Primer 2 (5′-ATA ACC TCC TCT CCT ATG GTA CCT AAA C-3′).”

should read:

“The linearized targeting construct was introduced by electroporation into C57BL/6-derived JN/2 recombinant embryonic stem cell (ESC) and neomycin-resistant clones were first screened for homologous recombination by PCR utilizing a pair of the following oligonucleotides: Primer 1 (5′-ATA TGT AGT GTC TGG TCA GGA TAA TAG TTG-3′) and Primer 3 (5′-ATA ACC TCC TCT CCT ATG GTA CCT AAA C-3′).”

“Transmission of the targeted allele was confirmed by PCR with Primer 1 and Primer 3 (5′-CTT TAT ATT TCA TTT TTG CTC AGG CTT C-3′). The mutant mice were cross-mated for more than nine generations with B6.FLIP mice to excise the flanked FRT sites by Flp-recombinase, and 8- to 12-week-old Cd103+/+ littermates were used as WT mice. Then, Cd103+/−  littermates were crossed to obtain homozygotes, and transmission of the targeted allele was confirmed by PCR with Primer 1 and Primer 3.”

should read:

“Transmission of the targeted allele was confirmed by PCR with Primer 1 and Primer 2 (5′-CTT TAT ATT TCA TTT TTG CTC AGG CTT C-3′). The mutant mice were cross-mated for more than nine generations with B6.FLIP mice to excise the flanked FRT sites by Flp-recombinase, and 8- to 12-week-old Cd103+/+ littermates were used as WT mice. Then, Cd103+/−  littermates were crossed to obtain homozygotes, and transmission of the targeted allele was confirmed by PCR with Primer 1 and Primer 2.”


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