Carbon to nitrogen (C/N) ratio of different biological hydrolysis-pretreated (BH-P) mixtures (w/w) of rice straw (RS) and Ulva spp. (U), and the hydrogen sulfide (H2S) content (n = 3, error bar = s.d.) of the biogas after their anaerobic co-digestion (ACD) using thalassic (TH) condition. The relationship between C/N () and actual methane yield could not be established due to the utilization of batch digester, which allowed longer retention time for the limited N to be recycled in the system. The decreasing amount of U coincided with the decreasing H2S content (), which was dictated by the amount of sulfate from the seawater in 100% RS along with the sulfate released from the hydrolysis of sulfated polysaccharides of U in 100% U, 75U:25RS, 50U:50RS, and 25U:75RS.