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Experimental Design of Metabolome, Signaling Molecules, and Transcriptome Analyses in EPS-Stimulated Differentiated C2C12 Myotubes
(A) Metabolome, signaling molecules, and transcriptome were measured during (0, 2, 5, 15, 30, and 60 min) and after (120, 240, and 420 min) electrical pulse stimulation (EPS) at 0, 2, and 20 Hz in differentiated C2C12 myotubes.
(B) Stimulation protocol of EPS.
