Haikun Song, Hexuan Li, Shimeng Guo, Yuyin Pan, Yuhua Fu, Zijian Zhou, Zhaoyang Li, Xue Wen, Xiaoli Sun, Bingqing He, Haifeng Gu, Quan Zhao, Cen Wang, Ping An, Shouqing Luo, Youhong Hu, Xin Xie, Boxun Lu. Targeting Gpr52 lowers mutant HTT levels and rescues Huntington’s disease-associated phenotypes. Brain 2018; 141: 1782–1798. doi:10.1093/brain/awy081.
The authors apologize for errors in Fig. 3C. The representative image of the D1 in situ staining of the brain slices from Gpr52−/−; HdhQ140/Q140 was incorrect. This error was due to mislabelling of cropped representative images when preparing the figure. In addition, the scale bar for Fig. 3C was incorrect, and should be 100 μm. All of the original experimental data are available and have been provided to the Editor of Brain. The representative image has now been corrected and is shown below. These errors do not affect the interpretation of this figure or the whole study.
Figure 3.
Knockout of Gpr52 reduced soluble and aggregated mHtt levels in striata of a knock-in Huntington’s disease mouse model. (A) Representative western blot and quantification of mHtt protein levels in striata and cortices from 16-month-old mice of indicated genotypes. Bars represent mean ± SEM. Statistical analysis was performed by one-way ANOVA and post hoc Dunnett tests. n.s. = P > 0.1, **P < 0.01. (B) Representative immunostaining and quantification of mHtt aggregates in striata slices from 10-month-old mice of indicated genotypes. Scale bar = 50 µm. Bars represent mean ± SEM; n indicates the number of different mice of each genotype. The image capture and analyses were performed blindly before annotating the genotypes. Aggregation signal per cell was analysed by particle analysis in ImageJ and calculated by: the number of aggregates × aggregate size × mean aggregate fluorescent intensity / cell number counted by DAPI. The statistical analysis was performed by one-way ANOVA and post hoc Dunnett tests for the indicated comparisons: ****P < 0.0001. (C) Representative in situ images and quantification of D1 or D2 positive neurons in striata slices from 16-month-old mice of indicated genotypes. Scale bar = 100 µm. Bars represent mean ± SEM; n indicates the number of different mice of each genotype. The statistical analysis was performed by one-way ANOVA and post hoc Bonferroni’s tests for the indicated comparisons: *P < 0.05, ****P < 0.0001.
The figure and figure legend have been corrected online.