Ghim M, Alpresa P, Yang SW, Braakman ST, Gray SG, Sherwin SJ, van Reeuwijk M, Weinberg PD. Visualization of three pathways for macromolecule transport across cultured endothelium and their modification by flow. Am J Physiol Heart Circ Physiol 313: H959–H973, 2017. First published July 28, 2017; doi:10.1152/ajpheart.00218.2017.—The lines in Fig. 11 were given the wrong sign and should have been flipped vertically, as shown in the revised figure below. Hence, the conclusion that cells align so as to minimize transWSS under these conditions is incorrect. (The cells do not align with the mean WSS vector either.) The conclusion that paracellular permeability correlates best with the magnitude of the minimized transverse WSS still stands because neither the data in Fig. 7, A and B, nor the statistics in discussion, paragraph 13, have changed as a result of the error. We speculate that some feature of the cell other than its long axis or its nucleus aligns so as to minimize transverse WSS, detects the resulting magnitude, and affects paracellular permeability.
Fig. 11.
The orientations of cells at different radial locations that would occur if the cells aligned with the mean WSS or aligned to obtain transWSSmin and the observed modal orientations of cells and their nuclei. These alignments are defined as for Fig. 9; −90 and +90° are equivalent since the two ends of the nuclei are indistinguishable. Data from the three locations nearest the center of the well were excluded since χ2-tests showed random cellular orientations in all three replicates, and the modal values are therefore determined by noise in these cases. (Directions defined by the shear metrics also become increasingly noisy toward the center.)