Table 3.

Summary of the effect of each mutation on the relative stability toward reduction, half-mAb formation, and Fab-arm exchange

IgG4 Hinge Region Mutation(s)	Increased Stability Toward Reduction	Decrease in half-mAb Formation	Decrease in Fab-arm Exchange
Y219C	±	+++	++
G220C	+	++	+++
S228P	+	−	+
Y219C+ S228P	+	+++	+++
G220C+ S228P	++	++	+++
Y219C+ G220C	++	+	++
Y219C+ G220C+ S228P	+++	+++	+++