Figure 2.
(a) Daudi cells were incubated with FITC-RTX in the concentration range from 7 to 60 nM until binding levels are close to equilibrium followed by replacement with the same concentration of unlabeled RTX to observe the release of bound FITC-RTX. (b) Interaction Map analysis for the binding traces displayed in (a). With increasing concentrations, the interaction shifts to the right on the x-axis, indicating that RTX binding becomes less stable with increasing concentrations.