Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Sep 14;10(24):11339–11358. doi: 10.7150/thno.48225

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The author(s)

This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.

PMC Copyright notice

TSTA3 silencing reduced ESCC cells invasion and fucosylation level. (A) Relative TSTA3 mRNA levels in KYSE510 (left) and KYSE180 (right) cells treated with the lentivirus expressing TSTA3-shRNA (sh1 or sh2) or sh-NC (negative control) were assessed by qPCR. (B) Relative expression of TSTA3 protein in KYSE510 (left) and KYSE180 (right) cells treated with the lentivirus expressing TSTA3-shRNA (sh1 or sh2) or sh-NC were assessed by western blot. (C) Transwell invasion assays on KYSE510 (upper) and KYSE180 (bottom) with TSTA3 knockdown and control group. Right is a bar chart for quantitative analysis. (D) UEA-I fluorescence of KYSE180 (upper) and KYSE510 (bottom) cells was analyzed by FACS. The right panel shows quantitation of UEA-I fluorescence. All data are presented as the mean ± standard deviation and three independent experiments. (E) Representative immunohistochemistry images of TSTA3 expression in tumor tissues, matched normal tissues and metastatic lymph node from paraffin-embedded formalin-fixed 11 ESCC tissues. Right: TSTA3 expression in metastatic lymph node tissue showed significantly higher expression than that of matched tumor and normal tissues.