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. 2020 Oct 5;9(10):87. doi: 10.1038/s41389-020-00272-0

Fig. 7. WAVE3 phosphorylation regulates the interplay between WAVE3 and PI3K, TGF-β, and EGF signaling pathways in vivo.

Fig. 7

Representative confocal microscopic images of tumor sections stained for pAKT-S473 (red) of MDA-MB-231 (a) and 4T1 (b) and their derivatives. Size bar, 75 µm. Cell nuclei were counterstained with DAPI (blue). b, d Quantification of pAKT-S473-positive areas in MDA-MB-231 (b) and 4T1 (d) tumors. Data are expressed as mean ± SEM. *,**p < 0.001, n = 5 mice. e, g Representative confocal microscopic images of tumor sections stained for pSMAD3 (Red) of MDA-MB-231 (e) and 4T1 (g) and their derivatives. Cell nuclei were counterstained with DAPI (blue). Size bar, 75 µm. f, h Quantification of pSMAD3-positive areas in MDA-MB-231 (f) and 4T1 (h) tumors. Data are expressed as mean ± SEM. *,**p < 0.001, n = 5 mice. i, k Representative confocal microscopic images of tumor sections stained for pERK1/2 (Red) of MDA-MB-231 (i) and 4T1 (k) and their derivatives. Cell nuclei were counterstained with DAPI (blue). Size bar, 75 µm. j, l Quantification of pERK1/2-positive areas in MDA-MB-231 (j) and 4T1 (l) tumors. Data are expressed as mean ± SEM. *,**p < 0.001, n = 5 mice. m, n Protein lysates from tumors derived from the parental (GFP) MDA-MB-231 (m) and 4T1 (n) cells, and their derivatives were analyzed by immunoblotting with the indicated antibodies. β-Actin was used as loading control. o Model describing the interplay between WAVE3, its phosphorylation, and the PI3K/TGF-β/EGF signaling pathways in regulation of tumor growth in BC.