Table 5.
[Crowder] (w/v) | Cyt. c reduction (min−1) |
---|---|
0 % | 1403 ± 49 |
3% Ficoll 70 | 1510 ± 38 |
7% Ficoll 70b | 1668 ± 70 |
11% Ficoll 70 | 1795 ± 108 |
14% Ficoll 70c | 1928 ± 130 |
4.6% Dextran 70b | 1680 ± 88 |
7.5% Dextran 70c | 1947 ± 122 |
Rates are the average of at least three assays. The final concentration of human iNOS holoenzyme is 1.5 nM.
The solution viscosity with the added 7 % Ficoll 70 or 4.6 % Dextran 70 is the same (2.7 cP).
The solution viscosity with the added 14 % Ficoll 70 or 7.5% Dextran 70 is the same (5.0 cP).