Table 2.

Comparison of species ID by three-locus sequencing, RLB and RCA amongst eight Trichosporon species studied.

Strain ID by three-locus sequencing*	No. of isolates (IGS1 genotype*)	Strain ID by RLB	Strain ID by RCA
Trichosporon asahii	36 (4)	T. asahii	T. asahii
Trichosporon cutaneum	1 (1)	T. cutaneum	T. cutaneum
Trichosporon dermatis	1 (1)	T. dermatis†	T. dermatis
Trichosporon domesticum	4 (1)	T. domesticum	T. domesticum
Trichosporon inkin	1 (1)	T. inkin	T. inkin
Trichosporon japonicum	2 (2)	T. japonicum†	T. japonicum
Trichosporon jirovecii	2 (2)	T. jirovecii	T. jirovecii
Trichosporon laibachii	1 (1)	T. laibachii	T. laibachii

Abbreviations: ID, identification; IGS1, intergenic spacer 1 region; RLB, reverse line blot hybridization assay; RCA, rolling circle amplification. *Genotypes within species were further assigned based on IGS1 sequences as previously described [12]. †Identification of T. dermatis relied on obtaining a positive hybridization result with the group-specific probe which hybridizes with T. dermatis and T. jirovecii and subsequent absence of a RLB signal using a T. jirovecii-specific probe. Similarly, T. japonicum strains were first assigned to the T. asahii-T. japonicum group by hybridization with a combined T. asahii and T. japonicum group-specific probe and then as T. japonicum by absence of a probe signal with a T. asahii-specific probe (Figs. 1 and 2).