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. 2020 Sep 22;9:e55744. doi: 10.7554/eLife.55744

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© 2020, Felgner et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 1. — (A) Schematic depiction of the mono-flagellated, fully motile PA14 wild-type phenotype and three isogenic mutants which are non-motile due to the lack of flagella (ΔfliC), non-assembled flagellin (ΔflgK), or the lack of flagellar rotation (ΔmotABCD). (B) Representative pictures of the individual P. aeruginosa motility variants analyzed by scanning electron microscopy. (C) Swimming motility assessed on semisolid agar after 16 hr. Mean ± standard deviation is displayed of 16 biological replicates from two independent experiments. ***p<0.001, one-way analysis of variance (ANOVA) (post-hoc test: Dunnett). See also: Figure 1—source data 1.

Figure 1—source data 1. Bacterial strains and plasmids used in this study.

elife-55744-fig1-data1.xlsx^{(11.3KB, xlsx)}

Figure 1—source data 2. Scanning Electron microscopy.

elife-55744-fig1-data2.pdf^{(1.3MB, pdf)}

Figure 1—source data 3. Motility Plates.

elife-55744-fig1-data3.pdf^{(411.4KB, pdf)}