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1H NMR (500 MHz) analysis of the formation of main product α2,3‐SiaLac, side product Neu5Ac, and remaining substrate CMP‐Neu5Ac after 22 h of reaction. Full conversion was observed for parent 2,3SiaTpph, as well as A151D and S359T/S360T variants; for variants L387A and (A151D/L387A) 2.8 and 52.4 % of CMP‐Neu5Ac remained, respectively, due to incomplete conversion.
