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. Author manuscript; available in PMC: 2021 Aug 6.
Published in final edited form as: Cell. 2020 Jul 16;182(3):578–593.e19. doi: 10.1016/j.cell.2020.06.031

Figure 2. HFSC activity is modulated by ADRB2.

Figure 2.

(A) Expression of adrenergic receptors in HFSCs (RNA-seq). (B) Chromatin modifications around the loci of Adrb genes in HFSCs. (C) Schematic of K15-CrePGR activity (blue) and experimental design (arrow denotes harvesting). qRT-PCR of Adrb2 from FACS-purified HFSCs of control and K15-CrePGR; Adrb2 fl/fl (Adrb2-cKO) mice (n = 3 mice per condition). (D) H&E staining of control and Adrb2-cKO skin. Graph: hair cycle distribution (n = 5 – 7 mice per condition, 20 HF per mouse). (E) Topical application of procaterol (ADRB2 agonist) drives premature anagen entry (n = 10 mice per condition). Graph: back skin hair regrowth (%). (F) Colony formation assay on control and procaterol-treated human HFSCs. Graph: area covered by colonies (n = 3 – 5 wells per condition). Data are mean ± SEM. *: p<0.05; **: p<0.01; ***: p <0.001. See also Figure S2.