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. Author manuscript; available in PMC: 2021 Aug 6.
Published in final edited form as: Cell. 2020 Jul 16;182(3):578–593.e19. doi: 10.1016/j.cell.2020.06.031

Figure 7. SHH regulates APM development and sympathetic innervation to HFSCs.

Figure 7.

(A) Schematic: sequential development of hair follicles, APMs, and sympathetic innervations. (B) Immunofluorescent staining of ITGA8 and TH. Arrowheads: APMs; solid circles: dermal papilla. (C) LacZ and ITGA8 co-localization at P2 Gli1-LacZ skin. (D) ITGA8, H&E, and Masson trichrome staining of control and Pdgfra-Cre; Smo fl/fl (Smo-cKO) mice at P4. Graph: % of HFs with APMs (n = 3 mice per condition, 200 – 280 HF per mouse). (E) ITGA8 and TH immunofluorescent staining on control and Smo-cKO mice at P8. (F) Keratin 14 (K14) and ITGA8 immunofluorescent staining of control and K14-Cre; Shh fl/fl on P0 skin. (G) K14 and ITGA8 immunofluorescent staining of control and K14-Cre; Rosa-lsl-rtTA; TetO-P27 (K14-P27) mice on P4 skin. Graph: % of HFs with APMs (n = 2 mice per condition, 120 – 180 HF per mouse). (H) In situ hybridization of Shh in control and K14-P27 mice at P4. (I-J) Immunofluorescent staining of nephronectin (NPNT) in control, K14-Cre; Shh fl/fl (I) and SmocKO (J) mice. Data are mean ± SEM. *: p<0.05; **: p<0.01; ***: p <0.001. n.s.: not significant. See also Figure S6 and S7.