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. 2020 Sep 18;10(25):11562–11579. doi: 10.7150/thno.45459

Figure 6.

Figure 6

The deletion CCR2 in BMDMs abrogated the effect of KLF2-EVs on inhibiting BMDMs migration, and KLF2-EVs were localized in BM and internalized by macrophages. (A) Representative fluorescence staining of CCR2-siRNA (GFP green) in BMDMs. Scale bar=250 µm. Quantification of mRNA of CCR2 using qRT-PCR in CCR2-siRNA transduced BMDMs and NC-siRNA transduced BMDMs (n=5). (B) Representative images of BMDMs in Transwell experiment with treatment of KLF2-EVs or vector-EVs derived from BMDMs, and quantification of number of migrated cells (n=5). Scale bar=100 µm. (C) Representative flow cytometry plots and quantification showing percentage of cells in BM that absorbed PKH67-labeled EVs (n=7). (D) Representative images of the uptake of PKH67-labeled exosomes (green) by RAW264.7 cells (DAPI blue) and fluorescence uptake with PKH67 dye sample. Graphs depict mean ± SD. Statistical significance was measured via Student's t-test for two groups' comparison. ****P < 0.0001, ns = not significant.