Figure 5.

CM-derived sEVsmediate the eNOSactivation in CMECs through the p70s6k1 pathway. A-E) The levels of p-70s6k1, t-p70s6k1, p-eNOSand t-eNOSwere detected by Western Blot (n=4). F-G) Dead CMECs in flow cytometry assay (n=3). * P<0.05 vs. Control; # P <0.05 vs CM^TXL+NC. sEVs: small extracellular vesicles; CM: cardiomyocyte; CM^TXL: TXL-pretreated CMs; CM^TXL+siRNA group: CMECs were transfected with p70s6k1 siRNA before being co-cultured with CM^TXL and then exposed to H/R; CM^TXL+NC group: CMECs were transfected with negative control of siRNA before being co-cultured with CM^TXL and then exposed to H/R. All data are mean ±SEM. Statistical analysis was performed with one-way ANOVA followed by Tukey's test.