Table 1.

Different non CRISPR detection used for COVID-19 testing.

SN	Test	Description	References
1	Serological immunoassays	1) Rapid diagnostic test (RDT) (detection in 10 to 30 min) 2) Enzyme linked immunosorbent assay (ELISA) (detection in 1 to 5 h) 3) Neutralization assay (detection in 3 to 5 days).	[8], [12]
2	Real time RT-PCR	Real time RT-PCR detection is more sensitive and advantageous over reverse transcriptase (RT-PCR)	[13], [14]
3	Super high-speed qRT-PCR (SHRT-PCR)	Special rotating disk used for rotating samples to different set temperatures specific to each step of PCR, reducing the time required for temperature ramp up and ramp down time	[15]
4	TaqMan-based RT-PCR	TaqMan probes improve the sensitivity of assays	[13], [16]
5	Dual TaqMan probe based RT-PCR	2 probes specific for different sequences used for reducing the detection time with ultra-sensitivity	[17]
6	Multiplex RT-PCR	Four mismatch-tolerant molecular beacons targeting four gene used for detection to overcome the issue of rapid mutation in sequences	[13], [18]
7	RT-LAMP	Reverse transcription followed by as loop-mediated isothermal amplification. Abolishes the need of sophisticated instruments like thermal cycler as reaction is possible at single temperature by using water bath.	[10], [19]
8	RT-RPA	Reverse transcription followed by Recombinase polymerase amplification (RPA) which is also an isothermal amplification	[11]
9	Microarray based detection	Complementary probes coated on wells of plate utilized for detection	[20]