Figure 1.

GBM Tumor Tissues Contain Large Amounts of TG and LDs

(A) Thin-layer chromatography (TLC) analysis of total lipid extracts from the same weight (4 mg) of normal (N) versus tumor (T) tissues from GBM patient autopsy samples (middle panel). TG standard loaded on the left. The intensity of TLC TG bands for each sample was quantified by the ImageJ software and normalized to the average intensity of TG in six tumor tissues to determine the relative TG levels between normal brain and GBM tissue (mean ± SD, n = 6) (right panel). Significance was determined by unpaired Student's t test.

(B) Representative images of tumor tissues from GBM patient biopsy stained by H&E (left panel), BODIPY 493/503 (green), and DAPI (blue) (middle panel) or visualized by transmission electron microscopy (TEM) (right panel). Red arrows in TEM image indicate LDs. Scale bar: 50 μm for H&E, 10 μm for fluorescence imaging, 500 nm for TEM.

(C–F) Representative images of normal and tumor tissues from primary GBM57- (C) or U87-derived (E) orthotopic mouse models stained by H&E or BODIPY 493/503 (green)/DAPI (blue). Scale bar: 50 μm for H&E; 10 μm for fluorescence imaging. TLC analysis of total lipid extracts from normal and tumor tissues from GBM57 (D) or U87 (F) orthotopic mice. Relative TG levels were quantified by ImageJ software and normalized to the average TG levels in tumor tissues (mean ± SD, n = 4).

Significance was determined by an unpaired Student's t test. Please also see Figure S1A.