. 2020 Sep 15;11(1):1257–1267. doi: 10.1080/21505594.2020.1809329

Table 1.

Primers used for the detection of virulence genes and CRISPR-associated genes.

Gene	Primer	Sequence (5ʹ–3ʹ)	PCR product length (bp)	References
esp	espF	GGAACGCCTTGGTATGCTAAC	95	[46]
esp	espR	GCCACTTTATCAGCCTGAACC	95	[46]
cylA	cylF	ACTCGGGGATTGATAGGC	688	[47]
cylA	cylR	GCTGCTAAAGCTGCGCTT	688	[47]
hyl	hylF	ACAGAAGAGCTGCAGGAAATG	276	[11]
hyl	hylR	GACTGACGTCCAAGTTTCCAA	276	[11]
efaA	efaF	TGGGACAGACCCTCACGAATA	101	[48]
efaA	efaR	CGCCTGTTTCTAAGTTCAAGCC	101	[48]
gelE	gelF	TATGACAATGCTTTTTGGGAT	213	[47]
gelE	gelR	AGATGCACCCGAAATAATATA	213	[47]
ace	aceF	GGAGAGTCAAATCAAGTACGTTGGTT	101	[49]
ace	aceR	TGTTGACCACTTCCTTGTCGAT	101	[49]
ebpR	ebpF	AAAAATGATTCGGCTCCAGAA	101	[11]
ebpR	ebpR	TGCCAGATTCGCTCTCAAAG	101	[11]
asa1	asaF	GCACGCTATTACGAACTATGA	375	[47]
asa1	asaR	TAAGAAAGAACATCACCACGA	375	[47]
CRISPR1-cas csn1	For	CAGAAGACTATCAGTTGGTG	783	[18]
CRISPR1-cas csn1	Rev	CCTTCTAAATCTTCTTCATAG	783	[18]
CRISPR1-cas loci	For	GCGATGTTAGCTGATACAAC	315	[18]
CRISPR1-cas loci	Rev	CGAATATGCCTGTGGTGAAA	315	[18]
CRISPR2 loci	For	CTGGCTCGCTGTTACAGCT	variable	[18]
CRISPR2 loci	Rev	GCCAATGTTACAATATCAAACA	variable	[18]
CRISPR3-cas csn1	For	GCTGAATCTGTGAAGTTACTC	258	[18]
CRISPR3-cas csn1	Rev	CTGTTTTGTTCACCGTTGGAT	258	[18]
CRISPR3-cas loci	For	GATCACTAGGTTCAGTTATTTC	224	[18]
CRISPR3-cas loci	Rev	CATCGATTCATTATTCCTCCAA	224	[18]