Skip to main content
. 2020 Sep 14;9:e60482. doi: 10.7554/eLife.60482

Figure 2. tRNA binding to the 30S ribosomal subunit.

(A) Overall view of P-site tRNA anticodon stem-loop (ASL, cyan), mRNA (red), 16S rRNA nucleotides (light purple), and uS9 residues (gold). (B) Interactions between 30S subunit head nucleotide G1338 with P-tRNA ASL. (C) Interactions between 30S subunit head nucleotide A1339 with P-site ASL. (D) Interactions between P-tRNA ASL and protein uS9. Arg130 is observed stacking with nucleotide U33 of the ASL and forming hydrogen bonds with backbone phosphate groups. (E) Solvation of A-site tRNA near the 30S subunit head domain. A-site tRNA ASL in green, 16S rRNA in light purple, and mRNA in purple-red. Water oxygen atoms in red spheres and Mg2+ in green spheres. Maps shown in panels B–E are from the 30S subunit head-focused refinement.

Figure 2.

Figure 2—figure supplement 1. Solvation of the 30S ribosomal subunit.

Figure 2—figure supplement 1.

(A) Solvation of the 30S subunit with solvent oxygen atoms shown as red spheres. (B) Polyamine (gray carbon) and Mg2+ (green) sites in the 30S subunit, shown with metal-coordinating atoms (oxygen in red, nitrogen in blue). Cryo-EM density is shown with a low-pass filter of 8 Å.