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. 2020 Sep 10;12(9):2761. doi: 10.3390/nu12092761

Figure 6.

Figure 6

Choline acetyl transferase immunoreactive nerve fiber density in subendocardial areas of cardiac septum. (A) Representative photomicrographs of right side of the subendocardial area of the cardiac septum stained for ChAT, a marker for parasympathetic fibers (green). c—Control group; stz—diabetic group fed standard diet (both diets n6/n3 ratio ≈7); stz+DHA—Diabetic group supplemented with n3 PUFA (2.5% of fish oil, containing 16% eicosapentaenoic acid—EPA and 19% docosahexaenoic acid—DHA; n6/n3 ratio of ≈1); stz+n6—diabetic group fed with diet that contained 2.5% sunflower oil (n6/n3 ratio ≈60). (B) Threshold figures from A; white—Nerve fiber area. (C)—Neuronal density expressed as percentage of the tissue area. *—p < 0.05 difference between indicated groups. Scale-bar = 20 µm.