Figure 2.

Phylogenetic representation of Apostichopus californicus flavivirus-like contig 91. The tree was constructed by performing an alignment of overlapping regions with best BLASTx matches at NCBI using the CLC Sequence Viewer 8.0 native alignment algorithm. The tree is based on a ~420 amino acid alignment by neighbor joining and based on Jukes-Cantor distance. Values above nodes indicate bootstrap statistics (>50%) based on 1000 iterations. The green branches indicate the emerging aquatic and invertebrate-associated flavivirus clade [52]. An additional phylogenetic representation based on maximum likelihood is provided in the Supplementary Materials.