Numbers of virus-specific IgG and IgA antibody-secreting cell (ASC) in peripheral blood mononuclear cells (PBMC) (A,B), tracheobronchial lymph nodes (TBLN) (C,D), lymph nodes of the head (LNH) (E,F), and nasal mucosa (NMC) (G,H) for each individual pig after infection with NC95 and vaccination with PA10 followed by G08. Responses in the different tissues were examined at weeks 1, 2, 7, 8, 11, and 12 after infection. The first and second vaccinations were performed at weeks 7 and 11, respectively. Symbols represent responses of four individual pigs, which were examined at each time point. The mean numbers of ASC are shown by the bars. The different colors represent responses against the five different viruses (● NC95; ▲ PA10; ⯀ G08; ▼ VIC75; ◆ HK14). The asterisk (*) means that ASC responses were not examined at that particular time point.