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. 2020 Sep 2;12(9):976. doi: 10.3390/v12090976

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Figure A7 — Analysis of P. aeruginosa knockouts of identified unfoldases. (A) Markerless knockout mutants created in genetic background of P. aeruginosa rpoA::strep rac. Shown are PCRs with primers 600 bp up- and downstream target gene. (B) Microbiological characterization of single and double knockouts, with (left) and without (right) induction of the phage GNAT. (C) Affinity purifications of RNAP complex using the Strep-fusion at the C-terminus of the α RNAP subunit, with and without phage infection.