MitoSegNet Model Segmentation and Morphological Quantification Can Be Applied to Mitochondria of Mammalian Cells
Comparing untreated HeLa cells and HeLa cells treated with oligomycin or antimycin for a duration of 3 h.
(A) Visual comparison of untreated and treated mitochondrial morphology. Raw images are at the top and MitoSegNet model segmentations at the bottom. The scale bar is 2.5 μm.
(B) Average area, eccentricity, perimeter, and branch length of mitochondria were measured in segmented images of treated and untreated mitochondria. ∗p < 0.05, ∗∗∗p < 0.001 using an independent two-sample t test. N = 8.