Abstract
Background:
Autosomal recessive polycystic kidney disease (ARPKD) is a rare but potentially lethal genetic disorder typically characterized by diffuse renal microcysts. Clinical trials for patients with ARPKD are not currently possible due to the absence of sensitive measures of ARPKD kidney disease progression and/or therapeutic efficacy.
Methods:
In this study, animal and human MRI scanners were used to obtain quantitative kidney T1 and T2 relaxation time maps for both excised kidneys from bpk and wild type (WT) mice as well as for a pediatric patient with ARPKD and a healthy adult volunteer.
Results:
Mean kidney T1 and T2 relaxation times showed significant increases with age (p<0.05) as well as significant increases in comparison to WT mice (p<2 × 10−10). Significant or nearly significant linear correlations were observed for mean kidney T1 (p=0.030) and T2 (p=0.054) as a function of total kidney volume, respectively. Initial MRF assessments in a patient with ARPKD showed visible increases in both kidney T1 and T2 in comparison to the healthy volunteer.
Conclusions:
These preclinical and initial clinical MRI studies suggest that renal T1 and T2 relaxometry may provide an additional outcome measure to assess cystic kidney disease progression in patients with ARPKD.
INTRODUCTION
Autosomal recessive polycystic kidney disease (ARPKD) is a rare but potentially lethal and rapidly progressing genetic disorder that affects approximately 1/20,000 children (1,2). ARPKD is typically characterized by diffuse renal microcysts caused by dilatations of the collecting tubules (2). ARPKD kidney disease progression results in declining kidney function at an early age. Of the 70% of patients with ARPKD that survive through the neonatal period (3), 40% progress to end-stage renal disease (ESRD) by 15 years of age (1,3). Importantly, there are currently no disease-specific therapies for patients with ARPKD beyond kidney transplant and dialysis (1,2,4). As a result, clinical management of chronic kidney disease (CKD) and its complications remains the primary treatment option for patients with ARPKD (1,2,4).
Several novel therapies have been recently shown to ameliorate kidney disease in animal models of ARPKD and other related ciliopathies (1,5–9). However, a major roadblock for implementing clinical trials in patients with ARPKD is the absence of sensitive measures of ARPKD kidney disease progression and / or therapeutic efficacy (1,4). Unfortunately, serum creatinine naturally increases with age in children (10,11). In addition, eGFR is relatively insensitive, especially for early-stage disease, and may not accurately reflect the severity of kidney damage (10,11). Furthermore, in many rare diseases such as ARPKD, there is significant variability in rates of GFR decline over time. A prior study examining kidney disease progression in patients with ARPKD found that rates of GFR decline were relatively modest and also highly variable across the patient population (mean 6% / year, range 1–10% / year) (12). Therefore, a clinical need exists to develop a safe and sensitive measure for kidney disease progression, and eventually therapeutic efficacy, for patients with ARPKD.
Magnetic Resonance Imaging (MRI) can be used to provide high-resolution kidney images in both patients and animal models. Importantly, MRI is capable of providing images with a variety of soft tissue contrasts that require no ionizing radiation or injectable contrast agent (13). MRI assessments of total kidney volume (TKV) has already been established as a marker for kidney disease progression in patients with autosomal dominant PKD (ADPKD). Unfortunately, unlike ADPKD, TKV for patients with ARPKD is not a reliable marker of disease since kidney size stabilizes over time as the normal renal parenchyma is replaced by microcysts (1,14). Over the last decade, quantitative MRI techniques, such as diffusion MRI, arterial spin labeling (ASL), and MRI relaxometry (i.e., T1 and T2 mapping) have been used to noninvasively assess changes in renal microstructure, oxygenation, perfusion, and tissue composition for a variety of acute and chronic kidney diseases. However, most of these quantitative MRI techniques have not been thoroughly evaluated in either animal models or patients with ARPKD (1,15–19). In our initial work in the PCK rat model of ARPKD, we demonstrated that T2-weighted MRI reliably measured renal cystic burden with advancing cystic kidney disease (1). However, cystic kidney disease progression in the PCK rat is predominantly renal macrocysts. These macrocysts are in stark contrast to the diffuse renal microcysts typical of humans in ARPKD kidney disease. Therefore, in the current study we sought to evaluate the capability of T1 and T2 MRI to assess kidney progression in ex vivo kidneys from the bpk mouse model that exhibits diffuse renal cysts consistent with human ARPKD kidney disease, (9,20–22) with the overall hypothesis that kidney T1 and T2 MRI maps would provide sensitive assessments of cystic changes associated with progressive ARPKD kidney disease. In the second phase of this study, we obtained initial in vivo kidney T1 and T2 assessments in a patient with ARPKD and a healthy volunteer for comparison with the preclinical findings using a novel Magnetic Resonance Fingerprinting (MRF) technology.
METHODS
Preclinical MRI Assessments
Animal Models
All studies were conducted in accordance with approved Institutional Animal Care and use committee protocols at the Cleveland Clinic and Case Western Reserve University. Bpk mouse pups were euthanized at 8 (n=5), 14 (n=5), and 18 (n=3) days of age by exsanguination and the kidneys were excised and fixed in methanol. Kidneys from heterozygous age-matched littermates were also obtained as controls (8 (n=3), 14 (n=4), and 18 (n=5) days of age).
MRI Acquisitions
The preclinical MRI experiments were conducted on a 7.0 T Bruker Biospec small animal MRI scanner (Bruker, Inc., Billerica, MA). Both the left and right excised kidneys from each animal were scanned in centrifuge tubes filled with methanol. Each kidney sample was positioned with the kidney at isocenter in a 35-mm cylindrical transmit / receive volume coil to ensure uniform radiofrequency excitation. Axial T2-weighted images were acquired with a multi-echo spin echo acquisition to position the slices for the quantitative MRI scans. High-resolution coronal T1 and T2 maps were then obtained for each mouse kidney. T1 data was obtained using a conventional spin echo acquisition with multiple repetition times (TR = 10,000 ms, 5,000 ms, 2,000 ms, 1,000 ms, 500 ms, and 300 ms) (23). Additional T1 imaging parameters were: echo time (TE) = 10.4 ms, slice thickness (TH) = 1.0 mm, 12 slices, field of view (FOV) = 35 × 35 mm, in-plane resolution = 0.273 × 0.273 mm/pixel, number of signal averages (NSA) = 1. Total acquisition time for the T1 measurement was 39 minutes and 54 seconds. T2 data was acquired with a multi-echo spin echo acquisition (12 echoes, TE = 52 ms, 63 ms, 73 ms, 83 ms, 94 ms, 104 ms, 115 ms, 125 ms, 136 ms, 146 ms, 157 ms, and 167 ms) (24). Detailed T2 imaging parameters were: TR = 2,000 ms, TH = 1.0 mm, 12 slices, FOV = 35 × 35 mm, in-plane resolution = 0.273 × 0.273 mm/pixel. Total acquisition time for the T2 assessment was 17 minutes and 4 seconds.
Following completion of the preclinical MRI scans, representative kidneys from 8-dayold and 18-day-old bpk and age-matched WT littermates were paraffin-embedded. Five-micron sections centered at the renal hilum were obtained and stained with hematoxylin and eosin (H&E). Sections were visualized by light microscopy (2x magnification) and images captured using an attached digital camera and imported into Adobe Photoshop®.
Image and Statistical Analysis
Ex vivo MRI data was exported for offline analysis in Matlab (The Mathworks, Natick, MA). Quantitative T1 and T2 maps were obtained by voxel-wise linear least squared error fits of the imaging data to established mono-exponential models for magnetic relaxation (23,25,26). The resultant fits were visually inspected to ensure linearity of the data. A manual region-of-interest (ROI) analysis was performed on each imaging slice to calculate the mean and standard deviations for the T1 and T2 data for each kidney slice. The number of kidney voxels in each imaging slice was used to obtain weighted mean values over the set of imaging slices. An average of the left and right kidneys from each animal was completed. The total volume of each kidney was also calculated from the ROI analysis of the T2-weighted images.
Mean kidney T1 and T2 values for the bpk and control mice were compared across mouse strain and age using unpaired Student’s t-tests. Pearson correlations were used to determine relationships between the mean T1 and T2 values with kidney volume. A two-tailed probability of 0.05 (α) was used to test for significance.
Human Kidney MRI Assessments
All human imaging studies were performed in accordance with approved Institutional Review Board (IRB) protocols at the Cleveland Clinic and University Hospitals – Cleveland Medical Center. For this initial study, we recruited one adolescent patient with ARPKD and one young adult volunteer subject (age=18). The human MRI studies were conducted using a Siemens Skyra 3.0 tesla MRI scanner (Siemens Healthineers, Erlangen, Germany).
Human Kidney MRI Experiments
Each subject was scanned in the supine position using both a body array and spine array receiver coils to ensure uniform image quality over the subject’s kidneys. Initial localizer HASTE (Half-Fourier Acquisition Single-shot Turbo spin Echo) images were acquired during instructed breathholds in all three imaging planes to obtain anatomic images of both the left and right kidneys for accurate slice positioning in later acquisitions. T1 and T2 MRI data for each kidney was then was acquired for each kidney using a rapid Magnetic Resonance Fingerprinting (MRF) acquisition. The method as demonstrated by Chen, Y. et al. was adapted for use in the abdomen with the following parameters: TR = 5.74 ms, TE = 1.00 ms, flip angle range = 5–12°, TH = 6.0 mm, field of view (FOV) = 400 × 400 mm, in-plane resolution = 1.3 × 1.3 mm/pixel, 1728 MRF images, acquisition time = 15 seconds (27). Each MRF acquisition resulted in co-registered T1 and T2 maps for a single imaging slice (27). Each MRF imaging slice was positioned parallel to the long axis of each kidney. Three oblique coronal MRF images were acquired for both the left and right kidneys.
Human Image and Statistical Analysis
The human MRI data was exported for offline analysis in Matlab (The Mathworks, Natick, MA). Quantitative T1 and T2 maps for the MRF acquisitions were obtained by voxel-wise matching of the acquired MRF image profiles to the MRF dictionary as described previously (27,28). No other statistical calculations were completed for these proof-of-concept human studies.
RESULTS
Representative maps of renal T1 and T2 relaxation times as well as H&E-stained sections for an 18-day-old bpk mouse (A, B, C), an 8-day-old bpk mouse (D, E, F), and an 18-day-old WT mouse (G, H, I) are shown in Figure 1, respectively. Note the diffuse cystic architecture as well as the increased cystic burden and kidney volume for the bpk kidneys. The kidneys from the bpk mice also show visible increases in the renal T1 and T2 MRI values between 8-days-of-age and 18-days-of-age as expected due to cystic kidney disease progression.
Figure 1:
Representative ex vivo T1 and T2 relaxation time maps and corresponding H&E stained sections of (A,B,C) 18-day-old bpk mouse kidney, (D,E,F) 8-day-old bpk mouse kidney, and (G,H,I) 18-day-old WT mouse kidney. Note the visibly increased renal T1 and T2 values for the bpk kidneys over time in comparison to the WT kidney.
Mean kidney T1 and T2 values for each age group for both bpk and wild type mice plotted as a function of age are shown in Figure 2. The mean T1 and T2 values for the bpk kidneys were all significantly increased in comparison to the WT kidneys at all timepoints with respect to age (p < 2 × 10−10). For the bpk mice, significant increases in mean kidney T1 and T2 values were also observed for the older kidneys (T1: 8 vs. 18 days, p = 0.018; T2: 8 vs. 14 days, p = 0.0002 and 8 vs. 18 days, p = 0.0009). In contrast, the corresponding mean kidney values for the WT mice either decreased with age (T1: 8 vs. 18 days, p = 0.03) or did not change significantly (T2: all WT mice, p > 0.13). Histograms showing the voxel-wise compilation of kidney T1 and T2 values for bpk and WT control mice as a function of age are shown in Supplementary Figure 1.
Figure 2:
Comparison of mean renal A) T1 and B) T2 relaxation time for bpk and WT mice at 8 days, 14 days, 18 days of age. Bpk mouse kidneys exhibit progressive increases in T1 and T2 with age (*p<0.02). In contrast, WT mice show no change in T2 and a reduction in T1 with age (**p=0.03). Mean renal T1 and T2 values were also significantly increased for bpk mice in comparison to WT control mice at all ages (p < 2 × 10−10).
Mean kidney T1 and T2 values for bpk mice and WT control mice are also plotted as a function of total kidney volume (Figure 3 A, B). As expected, T1 and T2 values for the bpk kidneys increased with increasing kidney volume. The mean kidney T1 values for the bpk mice (n=13) resulted in a significant linear correlation with total kidney volume (R = 0.60, p = 0.030). Mean kidney T2 values trended towards a significant correlation with total kidney volume (T2: R = 0.55, p = 0.054). No significant correlations were observed between the MRI metrics and total kidney volume for the WT kidneys.
Figure 3:
Scatterplots of mean renal A) T1 and B) T2 relaxation time for bpk mice and WT mice as a function of total kidney volume. Mean renal T1 (R=0.60, *p=0.030) and T2 (R=0.55, p=0.054) show significant (T1) or nearly significant (T2) correlations with kidney volume. No significant correlations were observed for the WT mice.
Initial T1 and T2 maps for both the healthy young adult volunteer and the adolescent patient with ARPKD are shown in Figure 4. Note the obvious diffuse cystic architecture as well as the obvious size increases for the kidneys of the patient with ARPKD. The kidneys from the patient with ARPKD shows visible increases in T1 and T2 in comparison to the kidneys from the healthy volunteer. Overall, these initial human MRI assessments for ARPKD kidney disease are consistent with the preclinical imaging findings in Figures 1–3.
Figure 4:
Initial renal T1 (left column) and T2 (right column) relaxation time maps from a healthy young adult volunteer (top row) and a pediatric patient with ARPKD (bottom row). Note the visible increases in T1 and T2 for the patient with ARPKD consistent with the ex vivo bpk kidney MRI results.
DISCUSSION
In this study, we evaluated the capability of quantitative MRI techniques to assess cystic burden in ARPKD kidney disease. In particular, we evaluated the capability of mean kidney T1 and T2 MRI to quantify cystic kidney disease progression in a well-established ARPKD animal model that exhibits progressively increasing diffuse renal microcysts. We also performed initial proof-of-concept studies in a patient with ARPKD and a healthy volunteer. The mean kidney T1 and T2 for the bpk kidneys increased significantly with age consistent with histology and were significantly higher than for the WT kidneys (Figures 1 and 2). In addition, both of these MRI metrics exhibited significant (T1) or nearly significant (T2) correlations with total kidney volume (Figure 3). Most importantly, the initial human MRI results were consistent with these preclinical MRI assessments (Figure 4). Therefore, while additional longitudinal imaging studies in patients with ARPKD will need to be conducted to more fully evaluate the sensitivity and specificity of these MRI techniques in comparison to conventional clinical measures of kidney function (e.g., serum creatinine), these preclinical and initial clinical MRI results suggest that T1 and T2 MRI could be used to assess cystic kidney progression in patients with ARPKD.
The MRI methods used in this study have a number of advantages for eventual studies in patients with ARPKD. First, these MRI techniques are non-invasive and do not require any injectable MRI contrast agent making these methods safe for longitudinal studies for pediatric and adult patients with advanced kidney disease (13). Second, these MRI methods are quantitative and therefore provide an objective outcome measure that could be used in future clinical trials. Third, the clinical MRF techniques used herein require very short acquisition times (~15 seconds) resulting in reduced respiratory motion artifacts, potentially eliminating the need for deep sedation or general anesthesia for pediatric patients. Fourth, T1 and T2 MRI techniques are available on virtually all modern human MRI scanners. For example, T1 MRI assessments have been used in numerous MRI studies of the heart, lung, liver, and other organs (29–35). As such, these methods could be rapidly implemented in multi-center clinical trials to evaluate new therapies for ARPKD.
The MRF acquisition used to obtain the initial human kidney T1 and T2 maps shown in Figure 4 also provides multiple benefits for future human ARPKD studies. This methodology was initially described in 2013 and uses rapid a priori variation in the MRI acquisition parameters (e.g., excitation flip angle) to generate tissue-specific MRF signal evolution profiles. These profiles are then matched to the best-fitting theoretical signal evolution profile in the MRF dictionary to obtain simultaneous estimates of the T1 and T2 relaxation times for each image voxel. Multiple studies have previously shown that MRF is inherently resistant to motion artifacts and results in accurate and reproducible assessments of T1 and T2 relaxation times for brain, kidney, liver, and prostate imaging applications (27,36–39). For the MRF implementation used herein, we utilized a low tip angle MRF acquisition which provides accurate T1 and T2 estimates without the need for additional scans to compensate for B1 heterogeneities (27,38). The lower tip angles also limit the radiofrequency energy deposition to the subject. As this MRF acquisition was completed in a single 15-second breathhold for each imaging slice, the T1 and T2 relaxation time maps were free of respiratory motion artifacts. These T1 and T2 MRI assessments may also be combined with renal perfusion assessments or other imaging methodologies to more thoroughly characterize ARPKD kidney disease progression (34).
While these preclinical and initial clinical MRI studies show promise for the use of quantitative MRI methods to assess ARPKD kidney disease progression, this study also has several important limitations. The primary limitation for the preclinical portion of this study is that the bpk and WT kidneys were scanned ex vivo. It is well established that blood flow can significantly alter the in vivo kidney T1 and T2 values (40). As such, the addition of a renal blood MRI signal would likely alter the mean kidney T1 and T2 values for both the bpk and WT mice. However, while the absolute values for the ex vivo kidney T1 and T2 times may be somewhat different from in vivo values, the differences in T1 and T2 values between the bpk and WT mice as well as the changes in the kidney T1 and T2 values with age remain significant. Further, as the long-term goal of this study is to develop MRI markers of cystic kidney disease progression in patients with ARPKD, this ex vivo imaging approach allows these T1 and T2 MRI techniques to be initially evaluated in a very controlled environment with limited impact of known variables such as hydration status, cardiac output, and respiratory motion that can create confounds with the cystic disease information in the MRI data. Scanning these kidneys ex vivo also allows multiple MRI parameters (T1 and T2) to be acquired with very high resolution and accuracy. As such, the conventional spin echo methods used in this study to generate the ex vivo T1 and T2 maps for the bpk and WT kidneys require significant acquisition times (total scan time = ~1 hour) that are not practical for in vivo studies of bpk mice with advanced kidney disease. Ultimately, these ex vivo preclinical MRI results are consistent with both histology (Figure 1) as well as initial in vivo human MRI assessments (Figure 4). As such, these ex vivo MRI findings help support the notion that T1 and T2 MRI assessments may be useful to assess ARPKD kidney disease progression in patients.
The primary limitation for the clinical MRI studies is the small sample size. While additional patient scans are certainly needed, these proof-of-concept human imaging studies establish that obtaining high resolution, quantitative kidney T1 and T2 MRI maps in a practical timeframe with no sedation in a pediatric patient is feasible. Most importantly, these and any other MRI metrics will need to be compared with conventional measures of renal function (e.g., serum creatinine and albumin) in both cross-sectional and longitudinal imaging studies in order to evaluate the utility of these MRI techniques to assess ARPKD kidney disease progression in multi-center clinical trials.
In conclusion, this preclinical and initial clinical study suggests that MRI-based kidney T1 and T2 mapping could be used as a non-invasive imaging assessment of ARPKD kidney disease progression. Future longitudinal MRI / MRF studies in patients with ARPKD will further evaluate the sensitivity, specificity, and reproducibility of these MRI techniques to detect and stage ARPKD kidney disease. If successful, these non-invasive, quantitative MRI techniques could eventually be used as an outcome measure for clinical trials evaluating novel therapeutics aimed at limiting or preventing ARPKD kidney disease progression.
Supplementary Material
Impact Questions:
A major roadblock for implementing clinical trials in patients with ARPKD is the absence of sensitive measures of ARPKD kidney disease progression and / or therapeutic efficacy
A clinical need exists to develop a safe and sensitive measure for kidney disease progression, and eventually therapeutic efficacy, for patients with ARPKD
Mean kidney T1 and T2 MRI relaxation times showed significant increases with age (p<0.05) as well as significant increases in comparison to WT mice (p<2 × 10−10), indicating T1 and T2 may provide sensitive assessments of cystic changes associated with progressive ARPKD kidney disease
This preclinical and initial clinical study suggests that MRI-based kidney T1 and T2 mapping could be used as a non-invasive assessment of ARPKD kidney disease progression
These non-invasive, quantitative MRI techniques could eventually be used as an outcome measure for clinical trials evaluating novel therapeutics aimed at limiting or preventing ARPKD kidney disease progression
ACKNOWLEDGEMENTS
The authors would like to thank Michael Kavran and other staff of the Imaging Research Core at CWRU for their timely support.
Statement of financial support:
This research was supported by NIH / NIDDK R01 DK114425 and the PKD Foundation. In addition, this work utilized the imaging capabilities for the Imaging Research Core for the Case Comprehensive Cancer Center (P30 CA043703), the Digestive Diseases Research Core Center (P30 DK097948), and the Cystic Fibrosis Foundation Research Development Program.
Footnotes
Disclosure:
The authors declare no conflict of interest.
Category of Study:
Basic science.
Patient Consent:
All human imaging studies were performed in accordance with approved Institutional Review Board (IRB) protocols at the Cleveland Clinic and University Hospitals – Cleveland Medical Center. Informed written consent was obtained from all subjects before the MRI scans.
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