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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Virology. 2020 Aug 22;550:51–60. doi: 10.1016/j.virol.2020.08.003

Figure 3. Immunization with recombinant inactivated PR8 viruses containing chimeric HA induces IgG2a dominant isotype antibody specific for NAe and M2e.

Figure 3.

BALB/c mice (n=10) were intramuscularly (IM) immunized with recombinant A/PR8 influenza viruses containing NAe or M2e inserted into the HA head domain. Antigen-specific antibody responses in immune sera collected after second boost immunization were determined by ELISA. (A and B) Total IgG to NAe or M2e peptides. (C) Total IgG to A/PR8 virus. (D and E) IgG2a and IgG1 isotype antibodies against NAe or M2e peptides in 100-fold diluted immune sera harvested after second boost immunization. (F) IgG2a and IgG1 isotype antibodies to PR8 virus in 100-fold diluted boost immune sera. Statistical significance was determined by using two-way ANOVA. Data of optical density (OD) values are representative of individual animal out of two independent experiments. Error bars indicate the means ± SEM. ***; p<0.001.