FIG 7.
Effect of sugars on expression of P. infestans invertase genes. (A) qRT-PCR analysis of the invertases in young (nonsporulating) hyphae from modified Xu’s minimal medium containing 20 g/liter sucrose, glucose, or both sugars. Also shown are values for mycelia grown in sucrose but then starved for 6 h in medium lacking a carbon source. Expression values were calculated by the ΔΔCT method, using two constitutive genes as a control, and normalized to the samples containing only sucrose. (B) Same as panel A except that modified Henninger’s medium was used.