FIG 10.

GprM and GprJ are involved in the repression of several secondary metabolites (SMs). SMs were extracted from culture supernatants of strains grown for 120 h in glucose minimal medium before mass spectrometry (MS) was performed. (A to F) MS chromatograms of fumagillin (A), pyripyropene A (B), fumigaclavine C (C), fumitremorgin C (D), fumiquinazoline F (E), and fumiquinazoline C (F) generated for different strains. (G) Quantitative comparison of the SMs identified in panels A to F between the deletion and the wild-type strains. Standard deviations represent the average of biological triplicates with *, P < 0.05 in a two-tailed unpaired Student's t test.