In the original article, there was a mistake in Figure 3J and Figure 4R as published. We used the wrong flow plot of KO thymus in the upper panel of Figure 3J and Figure 4R due to an accidental error. The corrected Figure 3 and Figure 4 appear below.
Figure 3.
Loss of Akt2 reduces the proliferation and apoptosis of iNKT cells. (A) Flow cytometry of Ki-67 cells gated on iNKT cells in the thymus. (B,C) Percentage of Ki-67 cells in the thymus and spleen (n = 3 mice per group). (D) Flow cytometry of Ki-67 cells gated on NKT17 cells in the thymus. (E,F) Percentage of Ki-67 cells gated on NKT17 cells in the thymus (n = 3 mice per group) and spleen (n = 3 mice per group). (G) Flow cytometry of Annexin V cells gated on the iNKT in the thymus. (H,I) Percentage of Annexin V cells in the thymus and spleen of WT (n = 3), KO (n = 3), WT chimera mice (n = 4) and Akt2 KO chimera mice (n = 3). (J) Flow cytometry of Annexin V cells gated on the stage2 (CD24−CD44+NK1.1−) iNKT cells in the thymus of WT, KO, and chimera mice. (K,L) Percentage of Annexin V cells of stage2 iNKT cells in the thymus and spleen of WT (n = 3), KO (n = 3), WT chimera mice (n = 4), and Akt2 KO chimera mice (n = 3). (M) Flow cytometry of CD1d cells gated on 7AAD cells in the thymus. (N,O) Percentage of CD1d cells in the thymus (n = 3 mice per group) and spleen (n = 3 mice per group). (P) Flow cytometry of Bcl2 cells gated on iNKT cells in the thymus. (Q,R) Overlaid histograms show expression of Bcl2.chimerachimera *p < 0.05; **p < 0.01, Student's t-test.
Figure 4.
Akt2 regulates the NKT17 differentiation by promoting the expression of ICOS. (A–F) The percentages of ICOS of WT and Akt2 KO cells in thymus (n = 3 mice per group) and spleen (n = 3 mice per group) is shown. (C,F) Overlaid histograms show expression of ICOS in the thymus and spleen. (G–L) The percentages of ICOS of CD45.2+ cells in thymus and spleen of WT chimera mice (n = 4) and Akt2 KO chimera mice (n = 3) is shown. (I,L) Overlaid histograms show expression of ICOS in the thymus and spleen of chimeras. (M,N) The Flow cytometry of c-Maf and expression of c-Maf in the thymus. (O,P) Flow cytometry of IL-23R and expression of IL-23R in the thymus. (Q–S) mRNA levels of indicated molecules in WT and Akt2 KO iNKT cells. Expression of indicated mRNA from MACS- and FACS- sorted WT and Akt2 KO iNKT cells from freshly isolated thymocytes was quantified by real-time qPCR from three independent experiments. *p < 0.05; **p < 0.01, Student's t-test. (T) Flow cytometry analysis of the pSTAT3 for MFI in thymocytes from WT (n = 3) and Akt2 KO mice (n = 3). (U) Overlaid histograms show expression of pSTAT3 after α-GalCer stimulated 72 h. (V) Airway response to methacholine of WT (n = 3) and Akt2 KO mice (n = 3). LR, lung resistance from two independent experiments. *p < 0.05, Student's t-test.
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.